Anodic oxidation and hydrothermal treatment of commercially pure titanium surfaces increases expression of bone morphogenetic protein-2 in the adherent macrophage cell line J774A.1

Abstract The release of growth factors from platelets, mediated by the coagulation and the complement system, plays an important role in the bone formation around implants. This study aimed at exploring the thromboinflammatory response of H2O2-alkali soaked commercially pure titanium grade 2 discs exposed to whole human blood, as a way to assess the bioactivity of the discs. Commercially pure titanium grade 2 discs were modified by soaking in H2O2, NaOH and Ca(OH)2. The platelet aggregation, coagulation activation and complement activation was assessed by exposing the discs to fresh whole blood from human donors. The platelet aggregation was examined by a cell counter and the coagulation and complement activation were assessed by ELISA-measurements of the concentration of thrombin-antithrombin complex, C3a and terminal complement complex. The modified surface showed a statistically significant increased platelet aggregation, coagulation activation and complement activation compared to unexposed blood. The surface also showed a statistically significant increase of coagulation activation compared to PVC. The results of this study showed that the H2O2-alkali soaked surfaces induced a thromboinflammatory response that indicates that the surfaces are bioactive.

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Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets

Dentistry Journal ◽

10.3390/dj7040109 ◽

2019 ◽

Vol 7 (4) ◽

pp. 109 ◽

Cited By ~ 2

Author(s):

Tetsuhiro Tsujino ◽

Akira Takahashi ◽

Taisuke Watanabe ◽

Kazushige Isobe ◽

Yutaka Kitamura ◽

...

Keyword(s):

Industrial Development ◽

Platelet Adhesion ◽

Alveolar Bone ◽

Platelet Rich Plasma ◽

Pure Titanium ◽

Commercially Pure Titanium ◽

Commercially Pure ◽

Titanium Surfaces ◽

Cp Ti

Recent progress in the industrial development of dental implants has improved their surface bio-affinity, while clinical implantologists attempt to improve it through coating with various compounds, including platelet-rich plasma (PRP) in clinical settings. However, it is poorly understood how PRP acts on titanium surfaces. To validate this surface modification method and demonstrate how platelet-derived soluble biomolecules released from the activated adherent platelets act on plain, commercially pure-titanium (cp-Ti) plates, we evaluated the distribution of biomolecules by immunofluorescence. PPARγ, PDGF-B, and TGFβ1 were similarly released at immunofluorescence levels from activated adherent platelets, retained in the surrounding extra-platelet spaces for a while, and did not immediately diffuse away to distant spaces. Exogenously added CaCl2 augmented release and retention of those biomolecules along with activation and aggregation. Taken together with our previous data regarding platelet adhesion, these findings suggest that especially when treated with CaCl2, platelets immediately adhere on cp-Ti plates to release their stored biomolecules in the absence of plasma proteins and that these biomolecules do not diffuse away, but stay longer in extra-platelet spaces around the platelets by newly formed, immature fibrin fiber fragments. Consequently, these retained biomolecules are anticipated to cooperatively stabilize implants by stimulating alveolar bone regeneration and integration.

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Effects of the nanotopographic surface structure of commercially pure titanium following anodization–hydrothermal treatment on gene expression and adhesion in gingival epithelial cells

Materials Science and Engineering C ◽

10.1016/j.msec.2014.05.040 ◽

2014 ◽

Vol 42 ◽

pp. 273-279 ◽

Cited By ~ 1

Author(s):

J. Takebe ◽

K. Miyata ◽

S. Miura ◽

S. Ito

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Surface Structure ◽

Hydrothermal Treatment ◽

Pure Titanium ◽

Commercially Pure Titanium ◽

Commercially Pure ◽

Gingival Epithelial Cells

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Biomimetic coatings enhance tribocorrosion behavior and cell responses of commercially pure titanium surfaces

Biointerphases ◽

10.1116/1.4960654 ◽

2016 ◽

Vol 11 (3) ◽

pp. 031008 ◽

Cited By ~ 13

Author(s):

Isabella da Silva Vieira Marques ◽

Maria Fernanda Alfaro ◽

Miki Taketomi Saito ◽

Nilson Cristino da Cruz ◽

Christos Takoudis ◽

...

Keyword(s):

Pure Titanium ◽

Commercially Pure Titanium ◽

Cell Responses ◽

Commercially Pure ◽

Titanium Surfaces

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Improvement of tribological and anti-corrosive performance of titanium surfaces coated with dicationic imidazolium-based ionic liquids

RSC Advances ◽

10.1039/c6ra13961b ◽

2016 ◽

Vol 6 (82) ◽

pp. 78795-78802 ◽

Cited By ~ 9

Author(s):

Izabelle M. Gindri ◽

Danyal A. Siddiqui ◽

Clarissa P. Frizzo ◽

Marcos A. P. Martins ◽

Danieli C. Rodrigues

Keyword(s):

Ionic Liquids ◽

Amino Acid ◽

Pure Titanium ◽

Commercially Pure Titanium ◽

Commercially Pure ◽

Titanium Surfaces

In this work, dicationic imidazolium-based ionic liquids (ILs) with amino acid anionic moieties were employed as coatings for commercially pure titanium (Ti) surfaces.

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