Study of a novel three-dimensional scaffold to repair bone defect in rabbit

AbstractMethods to repair bone defects arising from trauma, resection, or disease, continue to be sought after. Cyclic mechanical loading is well established to influence bone (re)modelling activity, in which bone formation and resorption are correlated to micro-scale strain. Based on this, the application of mechanical stimulation across a bone defect could improve healing. However, if ignoring the mechanical integrity of defected bone, loading regimes have a high potential to either cause damage or be ineffective. This study explores real-time finite element (rtFE) methods that use three-dimensional structural analyses from micro-computed tomography images to estimate effective peak cyclic loads in a subject-specific and time-dependent manner. It demonstrates the concept in a cyclically loaded mouse caudal vertebral bone defect model. Using rtFE analysis combined with adaptive mechanical loading, mouse bone healing was significantly improved over non-loaded controls, with no incidence of vertebral fractures. Such rtFE-driven adaptive loading regimes demonstrated here could be relevant to clinical bone defect healing scenarios, where mechanical loading can become patient-specific and more efficacious. This is achieved by accounting for initial bone defect conditions and spatio-temporal healing, both being factors that are always unique to the patient.

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Effects of Velvet Antler Polypeptide-Collagen/Chitosan Materials on Proliferation of Osteoblast

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.380-384.4295 ◽

2013 ◽

Vol 380-384 ◽

pp. 4295-4298

Author(s):

Wen He Zhu ◽

Jun Jie Xu ◽

Wei Zhang ◽

Yan Li ◽

Xiao Jing Lu ◽

...

Keyword(s):

Drug Treatment ◽

Bone Defect ◽

Three Dimensional ◽

Chitosan Scaffold ◽

Release Drug ◽

Dimensional Network ◽

Velvet Antler ◽

Result Show ◽

Long Time ◽

Velvet Antler Polypeptide

A highly osteogenic hybrid bioabsorbable scaffold was developed for bone reconstruction. Though the use of a bioabsorbable collagen and chitosan scaffold for loading velvet antler polypeptide to repair bone defect and drug treatment. Velvet antler polypeptide and collagen were extracted for developing the compounded material. The SEM results show that the collagen and chitosan scaffold maintain the natural three dimensional network structures. The cell proliferation experiment result show that the can promote the osteoblast proliferation for a long time . These results indicated that this compound scaffold can sustainable to release drug and is a good material in bone defect and drug treatment.

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Three-dimensional-printed individualized porous implants: A new “implant-bone” interface fusion concept for large bone defect treatment

Bioactive Materials ◽

10.1016/j.bioactmat.2021.03.030 ◽

2021 ◽

Vol 6 (11) ◽

pp. 3659-3670

Author(s):

Teng Zhang ◽

Qingguang Wei ◽

Hua Zhou ◽

Zehao Jing ◽

Xiaoguang Liu ◽

...

Keyword(s):

Bone Defect ◽

Three Dimensional ◽

Large Bone Defect ◽

Bone Interface ◽

Fusion Concept ◽

Bone Defect Treatment ◽

Large Bone

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A Comparative Study of Bioartificial Bone Tissue Poly-L-lactic Acid/Polycaprolactone and PLLA Scaffolds Applied in Bone Regeneration

Journal of Nanomaterials ◽

10.1155/2014/935149 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 3

Author(s):

Weizong Weng ◽

Shaojun Song ◽

Liehu Cao ◽

Xiao Chen ◽

Yuanqi Cai ◽

...

Keyword(s):

Lactic Acid ◽

New Zealand ◽

Bone Regeneration ◽

Bone Tissue ◽

Bone Defect ◽

Mineral Density ◽

Group A ◽

Repair Bone Defect ◽

Group B ◽

New Zealand Rabbits

Bioartificial bone tissue engineering is an increasingly popular technique to repair bone defect caused by injury or disease. This study aimed to investigate the feasibility of PLLA/PCL (poly-L-lactic acid/polycaprolactone) by a comparison study of PLLA/PCL and PLLA scaffolds applied in bone regeneration. Thirty healthy mature New Zealand rabbits on which 15 mm distal ulna defect model had been established were selected and then were divided into three groups randomly: group A (repaired with PLLA scaffold), group B (repaired with PLLA/PCL scaffold), and group C (no scaffold) to evaluate the bone-remodeling ability of the implants. Micro-CT examination revealed the prime bone regeneration ability of group B in three groups. Bone mineral density of surgical site in group B was higher than group A but lower than group C. Meanwhile, the bone regeneration in both groups A and B proceeded with signs of inflammation for the initial fast degradation of scaffolds. As a whole, PLLA/PCL scaffoldsin vivoinitially degrade fast and were better suited to repair bone defect than PLLA in New Zealand rabbits. Furthermore, for the low mineral density of new bone and rapid degradation of the scaffolds, more researches were necessary to optimize the composite for bone regeneration.

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In Vivo Reconstruction of the Acetabular Bone Defect by the Individualized Three-Dimensional Printed Porous Augment in a Swine Model

BioMed Research International ◽

10.1155/2020/4542302 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Jun Fu ◽

Yi Xiang ◽

Ming Ni ◽

Xiaojuan Qu ◽

Yonggang Zhou ◽

...

Keyword(s):

Compressive Strength ◽

Elastic Modulus ◽

Bone Defect ◽

Bone Ingrowth ◽

Structural Parameters ◽

Three Dimensional ◽

Acetabular Bone ◽

Acetabular Bone Defect ◽

3D Printed ◽

Matching Degree

Background and Purpose. This study established an animal model of the acetabular bone defect in swine and evaluated the bone ingrowth, biomechanics, and matching degree of the individualized three-dimensional (3D) printed porous augment. Methods. As an acetabular bone defect model created in Bama miniswine, an augment individually fabricated by 3D print technique with Ti6Al4V powders was implanted to repair the defect. Nine swine were divided into three groups, including the immediate biomechanics group, 12-week biomechanics group, and 12-week histological group. The inner structural parameters of the 3D printed porous augment were measured by scanning electron microscopy (SEM), including porosity, pore size, and trabecular diameter. The matching degree between the postoperative augment and the designed augment was assessed by CT scanning and 3D reconstruction. In addition, biomechanical properties, such as stiffness, compressive strength, and the elastic modulus of the 3D printed porous augment, were measured by means of a mechanical testing machine. Moreover, bone ingrowth and implant osseointegration were histomorphometrically assessed. Results. In terms of the inner structural parameters of the 3D printed porous augment, the porosity was 55.48 ± 0.61 % , pore size 319.23 ± 25.05 μ m , and trabecular diameter 240.10 ± 23.50 μ m . Biomechanically, the stiffness was 21464.60 ± 1091.69 N / mm , compressive strength 231.10 ± 11.77 MPa , and elastic modulus 5.35 ± 0.23 GPa , respectively. Furthermore, the matching extent between the postoperative augment and the designed one was up to 91.40 ± 2.83 % . Besides, the maximal shear strength of the 3D printed augment was 929.46 ± 295.99 N immediately after implantation, whereas the strength was 1521.93 ± 98.38 N 12 weeks after surgery ( p = 0.0302 ). The bone mineral apposition rate (μm per day) 12 weeks post operation was 3.77 ± 0.93 μ m / d . The percentage bone volume of new bone was 22.30 ± 4.51 % 12 weeks after surgery. Conclusion. The 3D printed porous Ti6Al4V augment designed in this study was well biocompatible with bone tissue, possessed proper biomechanical features, and was anatomically well matched with the defect bone. Therefore, the 3D printed porous Ti6Al4V augment possesses great potential as an alternative for individualized treatment of severe acetabular bone defects.

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