Coaxial 3D bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

Kidney disease is an epidemic that affects more than 600 million people worldwide. The socioeconomic impacts of the disease disproportionately affect Hispanic and non-Hispanic Black Americans, making the disease an issue of social inequality. The urgency of this situation has only become worse during the COVID-19 pandemic, as those who are hospitalized for COVID-19 have an increased risk of kidney failure. For researchers, the kidney is a complex organ that is difficult to accurately model and understand. Traditional cell culture models are not adequate for studying the functional intricacies of the kidney, but recent experiments have offered improvements for understanding these systems. Recent progress includes organoid modeling, 3D bioprinting, decellularization, and microfluidics. Here, we offer a review of the most recent advances in kidney bioengineering.

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Practical Applications of 3D Cell Culture Biotechnologies for Translational Oncology and Personalized Therapy

Biotekhnologiya ◽

10.21519/0234-2758-2020-36-3-3-15 ◽

2020 ◽

Vol 36 (3) ◽

pp. 3-15

Author(s):

D.A. Chudakova ◽

E.Yu. Skorova ◽

I.V. Reshetov

Keyword(s):

Cell Culture ◽

Extracellular Matrix ◽

3D Culture ◽

3D Cell Culture ◽

Personalized Therapy ◽

3D Bioprinting ◽

Culture Systems ◽

Culture Models ◽

2D And 3D

The creation of in vitro three-dimensional cellular model systems (in vitro 3D cultures) is a fast-growing leading-edge segment of the biotechnological industry. We have examined in this work the key 80 articles published after 2008, and focused on applications of in vitro 3D culture in translational oncology. We described a broad range of 3D culture systems, including models with and without extracellular matrix (ECM). 3D culture models based on decellularized ECM were discussed in more detail. The role of ECM in pathogeneis of malignant neoplasms, in particular, in the phenomenon of the tumor resistance to chemotherapy, was evaluated. 2D and 3D culture systems were compared, and natural and synthetic ECM were described, as well as the model creation based on 3D bioprinting. Particular attention was paid to in vitro models of various cancers, including those at the metastatic stage, based on 3D cell cultures, which maximally mimic the in vivo tumor behavior. The prospects of the practical application of 3D cell culture models in preclinical drag screening and in personalized therapy were discussed. We also presented our data on in vitro 2D and 3D culturing of human cells on various substrates. 3D cellular models, 3D bioprinting, biotechnology, extracellular matrix, cancer, translational medicine, personalized medicine, drag development, in vitro, ex vivo, oncology The authors are grateful to Dr. E. Shabalina for providing part of the experimental data and to OKA-Biotech Company for the samples of recombinant Funding-The work was supported by a Grant from the Russian Science Foundation (no. 18-15-00391). doi: 10.21519/0234-2758-2020-36-3-3-15

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Treatment approaches combining cytostatic drugs with the oncolytic parvovirus H-1 increase cytotoxic effects in cell culture models of highly malignant pediatric brain tumors

Klinische Pädiatrie ◽

10.1055/s-0034-1393947 ◽

2014 ◽

Vol 226 (06) ◽

Author(s):

C Westphal ◽

S Öztürk ◽

R Josupeit ◽

B Leuchs ◽

O Witt ◽

...

Keyword(s):

Cell Culture ◽

Brain Tumors ◽

Pediatric Brain Tumors ◽

Cytostatic Drugs ◽

Cytotoxic Effects ◽

Treatment Approaches ◽

Culture Models ◽

Pediatric Brain ◽

Cell Culture Models

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Faculty Opinions recommendation of A 3D bioprinting system to produce human-scale tissue constructs with structural integrity.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726147454.793524547 ◽

2016 ◽

Author(s):

Milica Radisic

Keyword(s):

Structural Integrity ◽

3D Bioprinting ◽

Tissue Constructs ◽

Human Scale

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Motility Improvement of Biomimetic Trachea Scaffold via Hybrid 3D-Bioprinting Technology

Polymers ◽

10.3390/polym13060971 ◽

2021 ◽

Vol 13 (6) ◽

pp. 971

Author(s):

Young Soo Yu ◽

Chi Bum Ahn ◽

Kuk Hui Son ◽

Jin Woo Lee

Keyword(s):

Rotation Angle ◽

Thick Layer ◽

Cartilage Tissue ◽

Soft Tissue Reconstruction ◽

3D Bioprinting ◽

Rotating Beam ◽

Rotational Angle ◽

Gelatin Hydrogel ◽

Tissue Reconstruction ◽

Neck Rotation

A trachea has a structure capable of responding to various movements such as rotation of the neck and relaxation/contraction of the conduit due to the mucous membrane and cartilage tissue. However, current reported tubular implanting structures are difficult to impelement as replacements for original trachea movements. Therefore, in this study, we developed a new trachea implant with similar anatomical structure and mechanical properties to native tissue using 3D printing technology and evaluated its performance. A 250 µm-thick layer composed of polycaprolactone (PCL) nanofibers was fabricated on a rotating beam using electrospinning technology, and a scaffold with C-shaped cartilage grooves that mimics the human airway structure was printed to enable reconstruction of cartilage outside the airway. A cartilage type scaffold had a highest rotational angle (254°) among them and it showed up to 2.8 times compared to human average neck rotation angle. The cartilage type showed a maximum elongation of 8 times higher than that of the bellows type and it showed the elongation of 3 times higher than that of cylinder type. In cartilage type scaffold, gelatin hydrogel printed on the outside of the scaffold was remain 22.2% under the condition where no hydrogel was left in other type scaffolds. In addition, after 2 days of breathing test, the amount of gelatin remaining inside the scaffold was more than twice that of other scaffolds. This novel trachea scaffold with hydrogel inside and outside of the structure was well-preserved under external flow and is expected to be advantageous for soft tissue reconstruction of the trachea.

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A 3D Bioprinted Material That Recapitulates the Perivascular Bone Marrow Structure for Sustained Hematopoietic and Cancer Models

Polymers ◽

10.3390/polym13040480 ◽

2021 ◽

Vol 13 (4) ◽

pp. 480

Author(s):

Caitlyn A. Moore ◽

Zain Siddiqui ◽

Griffin J. Carney ◽

Yahaira Naaldijk ◽

Khadidiatou Guiro ◽

...

Keyword(s):

Breast Cancer ◽

Bone Marrow ◽

Translational Medicine ◽

Drug Response ◽

Drug Approval ◽

3D Models ◽

3D Bioprinting ◽

Effective Evaluation ◽

Experimental Systems ◽

Cancer Models

Translational medicine requires facile experimental systems to replicate the dynamic biological systems of diseases. Drug approval continues to lag, partly due to incongruencies in the research pipeline that traditionally involve 2D models, which could be improved with 3D models. The bone marrow (BM) poses challenges to harvest as an intact organ, making it difficult to study disease processes such as breast cancer (BC) survival in BM, and to effective evaluation of drug response in BM. Furthermore, it is a challenge to develop 3D BM structures due to its weak physical properties, and complex hierarchical structure and cellular landscape. To address this, we leveraged 3D bioprinting to create a BM structure with varied methylcellulose (M): alginate (A) ratios. We selected hydrogels containing 4% (w/v) M and 2% (w/v) A, which recapitulates rheological and ultrastructural features of the BM while maintaining stability in culture. This hydrogel sustained the culture of two key primary BM microenvironmental cells found at the perivascular region, mesenchymal stem cells and endothelial cells. More importantly, the scaffold showed evidence of cell autonomous dedifferentiation of BC cells to cancer stem cell properties. This scaffold could be the platform to create BM models for various diseases and also for drug screening.

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A simple route to functionalising electrospun polymer scaffolds with surface biomolecules

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2021.120231 ◽

2021 ◽

Vol 597 ◽

pp. 120231

Author(s):

Karolina Dziemidowicz ◽

Steve Brocchini ◽

Gareth R. Williams

Keyword(s):

Polymer Scaffolds ◽

Simple Route

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3D-Bioprinting Strategies Based on In Situ Bone-Healing Mechanism for Vascularized Bone Tissue Engineering

Micromachines ◽

10.3390/mi12030287 ◽

2021 ◽

Vol 12 (3) ◽

pp. 287

Author(s):

Ye Lin Park ◽

Kiwon Park ◽

Jae Min Cha

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Bone Healing ◽

Critical Size ◽

Current Knowledge ◽

3D Bioprinting ◽

The Past ◽

Regeneration Processes

Over the past decades, a number of bone tissue engineering (BTE) approaches have been developed to address substantial challenges in the management of critical size bone defects. Although the majority of BTE strategies developed in the laboratory have been limited due to lack of clinical relevance in translation, primary prerequisites for the construction of vascularized functional bone grafts have gained confidence owing to the accumulated knowledge of the osteogenic, osteoinductive, and osteoconductive properties of mesenchymal stem cells and bone-relevant biomaterials that reflect bone-healing mechanisms. In this review, we summarize the current knowledge of bone-healing mechanisms focusing on the details that should be embodied in the development of vascularized BTE, and discuss promising strategies based on 3D-bioprinting technologies that efficiently coalesce the abovementioned main features in bone-healing systems, which comprehensively interact during the bone regeneration processes.

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