scholarly journals Genomic Regions Targeted by DNA Topoisomerase IIβ Frequently Interact With a Nuclear Scaffold/Matrix Protein hnRNP U/SAF‐A/SP120

2015 ◽  
Vol 116 (4) ◽  
pp. 677-685 ◽  
Author(s):  
Mary Miyaji ◽  
Ryohei Furuta ◽  
Kuniaki Sano ◽  
Kimiko M. Tsutsui ◽  
Ken Tsutsui
Keyword(s):  
Matrix Protein ◽  
Dna Topoisomerase ◽  
Nuclear Scaffold ◽  
Hnrnp U ◽  
Genomic Regions

scholarly journals Regulation of DNA Topoisomerase IIβ through RNA-dependent Association with Heterogeneous Nuclear Ribonucleoprotein U (hnRNP U)

2010 ◽  
Vol 285 (34) ◽  
pp. 26451-26460 ◽  
Author(s):  
Shinji Kawano ◽  
Mary Miyaji ◽  
Shoko Ichiyasu ◽  
Kimiko M. Tsutsui ◽  
Ken Tsutsui
Keyword(s):  
Dna Topoisomerase ◽  
Heterogeneous Nuclear Ribonucleoprotein ◽  
Hnrnp U ◽  
Nuclear Ribonucleoprotein

Nucleic-acid-binding properties of hnRNP-U/SAF-A, a nuclear-matrix protein which binds DNA and RNA in vivo and in vitro

1994 ◽  
Vol 221 (2) ◽  
pp. 749-757 ◽  
Author(s):  
Frank O. FACKELMAYER ◽  
Kirsten DAHM ◽  
Andrea RENZ ◽  
Uwe RAMSPERGER ◽  
Arndt RICHTER
Keyword(s):  
Nucleic Acid ◽  
Nuclear Matrix ◽  
Matrix Protein ◽  
Nucleic Acid Binding ◽  
Nuclear Matrix Protein ◽  
Binding Properties ◽  
Dna And Rna ◽  
Hnrnp U

scholarly journals The Matrix Protein hnRNP U Is Required for Chromosomal Localization of Xist RNA

2010 ◽  
Vol 19 (3) ◽  
pp. 469-476 ◽  
Author(s):  
Yuko Hasegawa ◽  
Neil Brockdorff ◽  
Shinji Kawano ◽  
Kimiko Tsutui ◽  
Ken Tsutui ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Chromosomal Localization ◽  
Xist Rna ◽  
Hnrnp U ◽  
The Matrix

scholarly journals Dynamic localization of DNA topoisomerase 1 and its functional relevance during Drosophila development

2021 ◽  
Author(s):  
Wuqiang Huang ◽  
Zhiping Liu ◽  
Yikang S Rong
Keyword(s):  
Topoisomerase I ◽  
Fruit Fly ◽  
Rdna Locus ◽  
Live Cells ◽  
Dna Topoisomerase ◽  
Topoisomerase 1 ◽  
Functional Relevance ◽  
Diploid Cells ◽  
Eukaryotic Organisms ◽  
Genomic Regions

Abstract DNA topoisomerase I (Top1) maintains chromatin conformation during transcription. While Top1 is not essential in simple eukaryotic organisms such as yeast, it is required for the development of multicellular organisms. In fact, tissue and cell type specific functions of Top1 have been suggested in the fruit fly Drosophila. A better understanding of Top1’s function in the context of development is important as Top1 inhibitors are among the most widely used anti-cancer drugs. As a step towards such a better understanding, we studied its localization in live cells of Drosophila. Consistent with prior results, Top1 is highly enriched at the nucleolus in transcriptionally active polyploid cells, and this enrichment responds to perturbation of transcription. In diploid cells, we uncovered evidence for Top1 foci formation at genomic regions not limited to the active rDNA locus, suggestive of novel regulation of Top1 recruitment. In the male germline, Top1 is highly enriched at the paired rDNA loci on sex chromosomes suggesting that it might participate in regulating their segregation during meiosis. Results from RNAi mediated Top1 knock-down lend support to this hypothesis. Our study has provided one of the most comprehensive description of Top1 localization during animal development.

scholarly journals The Glucocorticoid Receptor Is Associated with the RNA-binding Nuclear Matrix Protein hnRNP U

1997 ◽  
Vol 272 (45) ◽  
pp. 28471-28478 ◽  
Author(s):  
Martin Eggert ◽  
Jörg Michel ◽  
Sandra Schneider ◽  
Harald Bornfleth ◽  
Aria Baniahmad ◽  
...  
Keyword(s):  
Glucocorticoid Receptor ◽  
Nuclear Matrix ◽  
Matrix Protein ◽  
Rna Binding ◽  
Nuclear Matrix Protein ◽  
Hnrnp U

Analysis of genomic regions recognized in vivo by DNA topoisomerase II in the developing cerebellum

1998 ◽  
Vol 31 ◽  
pp. S285
Author(s):  
Kuniaki Sano ◽  
Ken Tsutsui ◽  
Kimiko Tsutsui ◽  
Shuji Seki ◽  
Akira Tokunaga
Keyword(s):  
Topoisomerase Ii ◽  
Dna Topoisomerase Ii ◽  
Dna Topoisomerase ◽  
Genomic Regions ◽  
Developing Cerebellum

Abstract P136: Dietary Salt and Non-Salt Components Have Substantial Effects on Genome-Wide DNA Methylation Patterns in Dahl SS Rats

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Justine M Abais-Battad ◽  
Pengyuan Liu ◽  
David L Mattson ◽  
Yong Liu ◽  
Allen W Cowley ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Matrix Protein ◽  
Regulation Of Gene Expression ◽  
Albumin Excretion Rate ◽  
Epigenetic Modifications ◽  
Differential Methylation ◽  
Dietary Salt ◽  
Genome Wide ◽  
Differentially Methylated Genes ◽  
Genomic Regions

Epigenetic modifications of the genome play a key role in the regulation of gene expression. It has been reported that epigenetic modifications of several genes are associated with hypertension. To investigate the potential role of genome-wide changes in DNA methylation in salt-induced hypertension, experiments were performed on inbred Dahl SS rats obtained from two colonies maintained at the Medical College of Wisconsin (i.e. MCWSS) and Charles River Laboratory (CRLSS). The colonies are genetically identical, but CRLSS rats were maintained on a whole grain diet containing 1% NaCl (CRLSS_LS) while MCWSS rats were fed casein-based AIN-76A chow containing 0.4% NaCl (MCWSS_LS) until both colonies were switched to an AIN-76A chow containing 4% NaCl for 14 days starting at 6 weeks of age (CRLSS_HS and MCWSS_HS, respectively). Mean arterial pressure and albumin excretion rate in MCWSS_HS rats were significantly greater (142±14 mmHg and 100±16 mg/day, n=6) than in CRLSS_HS rats (118±2 mmHg and 20±2 mg/day, n=7). Reduced representation bisulfite genome sequencing (RRBS) measured 5-Methylcytosine levels at single-base resolution in the renal outer medulla in the above groups, each with four biological replicates. For genomic regions located within CpG islands (CGI’s) and exhibiting differential methylation between LS and HS in each colony, HS diet increased median methylation levels several-fold in both MCWSS (7.45% vs. 0.35% for MCWSS_HS vs. MCWSS_LS, respectively, p = 2.84E-31) and CRLSS rats (7.62% vs. 1.21% for CRLSS_HS vs. CRLSS_LS, respectively, p = 1.65E-32). For genomic regions exhibiting differential methylation between MCWSS and CRLSS, MCWSS_HS rats (which exhibited higher blood pressure) had higher median methylation levels than CRLSS_HS rats (7.56% vs. 2.75%, p = 2.12E-9). We observed 156 hypermethylated and 241 hypomethylated regions within CGI’s of MCWSS_LS compared to CRLSS_LS. Examples of differentially methylated genes include the serine protease Prss2 , the transcription factor E2f1 , and the matrix protein Spock2 . These results suggest that sodium-dependent and independent dietary components could induce changes in DNA methylation that may predispose and participate in the development of hypertension and renal damage.

scholarly journals Different Concentrations of Mg++ Ions Affect Nuclear Matrix Protein Distribution During Thermal Stabilization of Isolated Nuclei

1997 ◽  
Vol 45 (10) ◽  
pp. 1317-1328 ◽  
Author(s):  
Luca M. Neri ◽  
S. Capitani ◽  
Aurelio Valmori ◽  
Beat M. Riederer ◽  
Alberto M. Martelli
Keyword(s):  
Nuclear Matrix ◽  
Matrix Protein ◽  
Thermal Stabilization ◽  
Cell Types ◽  
Nuclear Bodies ◽  
Immunofluorescent Staining ◽  
Protein Distribution ◽  
Nuclear Scaffold ◽  
Isolated Nuclei ◽  
Confocal Scanning Laser Microscope

The nuclear matrix, a proteinaceous network believed to be a scaffolding structure determining higher-order organization of chromatin, is usually prepared from intact nuclei by a series of extraction steps. In most cell types investigated the nuclear matrix does not spontaneously resist these treatments but must be stabilized before the application of extracting agents. Incubation of isolated nuclei at 37C or 42C in buffers containing Mg++ has been widely employed as stabilizing agent. We have previously demonstrated that heat treatment induces changes in the distribution of three nuclear scaffold proteins in nuclei prepared in the absence of Mg++ ions. We studied whether different concentrations of Mg++ (2.0–5 mM) affect the spatial distribution of nuclear matrix proteins in nuclei isolated from K562 erythroleukemia cells and stabilized by heat at either 37C or 42C. Five proteins were studied, two of which were RNA metabolism-related proteins (a 105-kD component of splicing complexes and an RNP component), one a 126-kD constituent of a class of nuclear bodies, and two were components of the inner matrix network. The localization of proteins was determined by immunofluorescent staining and confocal scanning laser microscope. Mg++ induced significant changes of antigen distribution even at the lowest concentration employed, and these modifications were enhanced in parallel with increase in the concentration of the divalent cation. The different sensitivity to heat stabilization and Mg++ of these nuclear proteins might reflect a different degree of association with the nuclear scaffold and can be closely related to their functional or structural role.

Periodic Structure in the Matrix Protein of an Insect Virus

1982 ◽  
Vol 40 ◽  
pp. 302-303
Author(s):  
H.M. Mazzone ◽  
W.F. Engler ◽  
R. Zerillo ◽  
G.F. Bahr
Keyword(s):  
Inclusion Body ◽  
Periodic Structure ◽  
Matrix Protein ◽  
Malacosoma Disstria ◽  
Insect Virus ◽  
Virus Particles ◽  
The Matrix ◽  
Nucleopolyhedrosis Virus

The nucleopolyhedrosis virus (NPV) of the forest tent cater - pillar (Malacosoma disstria Hubner) has been analyzed in our laboratories. As a representative of the Baculovirus class, the NPV has virus particles enclosed with in a proteinaceous structure, the inclusion body.

The in vivo distribution and dynamics of DNA topoisomerase II in Drosophila embryonic nuclei and chromosomes

1993 ◽  
Vol 51 ◽  
pp. 74-75
Author(s):  
Jason R. Swedlow ◽  
Neil Osheroff ◽  
Tim Karr ◽  
John W. Sedat ◽  
David A. Agard
Keyword(s):  
Topoisomerase Ii ◽  
Biochemical Characterization ◽  
Developmental Cycle ◽  
Dna Topoisomerase Ii ◽  
Chromosomal Distribution ◽  
Dna Topoisomerase ◽  
Ideal System ◽  
Dnase Treatment

DNA topoisomerase II is an ATP-dependent double-stranded DNA strand-passing enzyme that is necessary for full condensation of chromosomes and for complete segregation of sister chromatids at mitosis in vivo and in vitro. Biochemical characterization of chromosomes or nuclei after extraction with high-salt or detergents and DNAse treatment showed that topoisomerase II was a major component of this remnant, termed the chromosome scaffold. The scaffold has been hypothesized to be the structural backbone of the chromosome, so the localization of topoisomerase II to die scaffold suggested that the enzyme might play a structural role in the chromosome. However, topoisomerase II has not been studied in nuclei or chromosomes in vivo. We have monitored the chromosomal distribution of topoisomerase II in vivo during mitosis in the Drosophila embryo. This embryo forms a multi-nucleated syncytial blastoderm early in its developmental cycle. During this time, the embryonic nuclei synchronously progress through 13 mitotic cycles, so this is an ideal system to follow nuclear and chromosomal dynamics.

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