Effects of calcium dobesilate (CaD) interference on serum creatinine measurements: a national External Quality Assessment (EQA)-based educational survey of drug-laboratory test interactions

AbstractObjectivesDrug-laboratory test interactions (DLTIs) are one of the major sources of laboratory errors. Calcium dobesilate (CaD) interference on serum creatinine testing is a widespread problem that has long been ignored in China. A national EQA-based survey was launched to investigate the current status of CaD interference on creatinine routine methods used in China and enhance the education of CaD interference in clinical laboratories.MethodsA descriptive survey was developed to characterize the status quo of Chinese laboratory professionals’ cognition to CaD interference. Four of survey samples which were spiked with/without interference additive were shipped to 175 participant laboratories. The target reference values from a reference measurement procedure were compared against the results from participating laboratories to evaluate the CaD interference on serum creatinine measurements using enzymatic method or Jaffé method.ResultsThe lack of knowledge of DLTIs and the barriers to collect information from pharmacological and laboratory data systems had become the main problems on implementing DLTIs education in China. A significant negative influence of CaD on enzymatic method was observed regardless of measurement platforms. Jaffé method was generally free from interaction with CaD but showed poor precision and accuracy at low creatinine concentrations.ConclusionsMore efforts should be made to enhance the education of DLTIs in clinical laboratories in China.

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Experience of symptom control, anxiety and associating factors in a palliative care unit evaluated with Support Team Assessment Schedule Japanese version

Scientific Reports ◽

10.1038/s41598-021-97143-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Tetsuya Ito ◽

Emi Tomizawa ◽

Yuki Yano ◽

Kiyozumi Takei ◽

Naoko Takahashi ◽

...

Keyword(s):

Palliative Care ◽

Symptom Control ◽

Laboratory Data ◽

Japanese Version ◽

Palliative Care Unit ◽

Current Status ◽

Physical Factors ◽

Patient Anxiety ◽

Team Assessment ◽

Support Team

AbstractVarious physical and psychosocial difficulties including anxiety affect cancer patients. Patient surroundings also have psychological effects on caregiving. Assessing the current status of palliative care intervention, specifically examining anxiety and its associated factors, is important to improve palliative care unit (PCU) patient quality of life (QOL). This study retrospectively assessed 199 patients admitted to a PCU during August 2018–June 2019. Data for symptom control, anxiety level, disease insight, and communication level obtained using Support Team Assessment Schedule Japanese version (STAS-J) were evaluated on admission and after 2 weeks. Palliative Prognostic Index (PPI) and laboratory data were collected at admission. Patient anxiety was significantly severer and more frequent in groups with severer functional impairment (p = 0.003) and those requiring symptom control (p = 0.006). Nevertheless, no relation was found between dyspnea and anxiety (p = 0.135). Patients with edema more frequently experienced anxiety (p = 0.068). Patient survival was significantly shorter when family anxiety was higher after 2 weeks (p = 0.021). Symptoms, edema, and disabilities in daily living correlate with patient anxiety. Dyspnea is associated with anxiety, but its emergence might be attributable mainly to physical factors in this population. Family members might sensitize changes reflecting worsened general conditions earlier than the patients.

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Quality Indicators of the Pre-Analytical Phase

Journal of Medical Biochemistry ◽

10.2478/v10011-012-0013-2 ◽

2012 ◽

Vol 31 (3) ◽

pp. 174-183 ◽

Cited By ~ 2

Author(s):

Nada Majkić-Singh ◽

Zorica Šumarac

Keyword(s):

Health Care ◽

Patient Safety ◽

Quality Indicators ◽

Task Force ◽

Improve Patient Safety ◽

Laboratory Data ◽

Sample Collection ◽

Laboratory Errors ◽

Analytical Phase ◽

Total Testing Process

Quality Indicators of the Pre-Analytical PhaseQuality indicatorsare tools that allow the quantification of quality in each of the segments of health care in comparison with selected criteria. They can be defined as an objective measure used to assess the critical health care segments such as, for instance, patient safety, effectiveness, impartiality, timeliness, efficiency, etc. In laboratory medicine it is possible to develop quality indicators or the measure of feasibility for any stage of the total testing process. The total process or cycle of investigation has traditionally been separated into three phases, the pre-analytical, analytical and post-analytical phase. Some authors also include a »pre-pre« and a »post-post« analytical phase, in a manner that allows to separate them from the activities of sample collection and transportation (pre-analytical phase) and reporting (post-analytical phase). In the year 2008 the IFCC formed within its Education and Management Division (EMD) a task force calledLaboratory Errors and Patient Safety (WG-LEPS)with the aim of promoting the investigation of errors in laboratory data, collecting data and developing a strategy to improve patient safety. This task force came up with the Model of Quality Indicators (MQI) for the total testing process (TTP) including the pre-, intra- and post-analytical phases of work. The pre-analytical phase includes a set of procedures that are difficult to define because they take place at different locations and at different times. Errors that occur at this stage often become obvious later in the analytical and post-analytical phases. For these reasons the identification of quality indicators is necessary in order to avoid potential errors in all the steps of the pre-analytical phase.

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LC-MS/MS Method for Serum Creatinine: Comparison with Enzymatic Method and Jaffe Method

PLoS ONE ◽

10.1371/journal.pone.0133912 ◽

2015 ◽

Vol 10 (7) ◽

pp. e0133912 ◽

Cited By ~ 12

Author(s):

Meixian Ou ◽

Yunxiao Song ◽

Shuijun Li ◽

Gangyi Liu ◽

Jingying Jia ◽

...

Keyword(s):

Serum Creatinine ◽

Enzymatic Method

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Assessment of biochemistry request forms

Journal of Chitwan Medical College ◽

10.3126/jcmc.v5i3.16521 ◽

2017 ◽

Vol 5 (3) ◽

pp. 18-24 ◽

Cited By ~ 1

Author(s):

S Singh ◽

S Khatiwada

Keyword(s):

Laboratory Data ◽

Tertiary Hospital ◽

Cross Sectional Study ◽

Successful Outcome ◽

Ethical Review ◽

Sample Type ◽

Cross Sectional ◽

Laboratory Errors ◽

Specimen Collection ◽

Drug History

Oversight of information on biochemistry requisition forms may lead to laboratory errors. The aim of this study was to evaluate the level of completion of these forms. The observational cross-sectional study was conducted between December 2014 and March 2015 in the biochemistry department of a tertiary hospital in Kathmandu after approval by the Institutional Ethical Review Committee. Two thousand and thirty nine request forms were randomly assessed for the completeness of information provided by the requesting physician. Microsoft excels software and SPSS-17 was used for analysis. Patient confidentiality was maintained. Out of 2039 request forms examined, the only 100% documented parameter was the patient’s name. Date of specimen collection was recorded in 79.74% of forms and age in 98.53%. The working diagnosis was recorded in 28.44% but no information regarding patient preparation. While the consultants name were stated in 13.29% of cases, drug history in 0.24%. Parameters like gender were recorded in 98.82%, sample type in 0.29%. Whether the patient was present in the ward or in the outpatient department was documented in 15.11% whereas patient number in 38.35%. Doctors were more likely to sign the forms rather than providing their name/designation. This study demonstrates that the custom of completion of request forms was poor. As laboratory data plays a significant role in medical diagnosis and research, incomplete data provided to the laboratory could significantly impact on the comments and successful outcome of treatment. Closer interaction between clinicians and laboratory personnel by means of request forms can improve the quality of services to patients.

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Kinetic Enzymatic Method for Determining Serum Creatinine

Clinical Chemistry ◽

10.1093/clinchem/21.10.1422 ◽

1975 ◽

Vol 21 (10) ◽

pp. 1422-1426 ◽

Cited By ~ 54

Author(s):

Gerald A Moss ◽

Richard J L Bondar ◽

Diane M Buzzelli

Keyword(s):

Serum Creatinine ◽

Accurate Determination ◽

Test Subject ◽

Rate Of Change ◽

Enzymatic Method ◽

Inhibitory Effects ◽

Standard Curve ◽

Analytical Recovery ◽

Enzymatic Procedure

Abstract Creatinine amidohydrolase is used to measure serum creatinine in a totally enzymatic procedure. Creatine, produced by hydrolysis, is acted upon by creatine kinase, and then by pyruvate kinase and lactate dehydrogenase, to result in a change in absorbance at 340 nm. The amount of creatinine present is related to the rate of change in A340 and is determined from a standard curve. Absorbance and concentration are linearly related to 100 mg/liter and only 250 µl of serum is required. At 1.0 g/liter, heparin, oxalate, citrate, ethylenediaminetetraacetate, ascorbate, or glucose had no significant effect on the accurate determination of creatinine; higher concentrations (30 g/liter) had inhibitory effects on the test. Analytical recovery of creatinine added to either normal or abnormal sera averaged 102%. When results of this procedure and of the standard direct Jaffé test were compared, the latter were significantly higher. Unlike the Jaffé method, the present method of determining creatinine is rapid (about 10 min per test), subject to few or no interfering substances, and requires no serum deproteinization.

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Markers for cardic damage - current status and future trends 7thBergmeyer Conference. IFCC-Roche Diagnostics Master Discussion Improving the Clinical Value of Laboratory Data

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.1080/00365519909168320 ◽

1999 ◽

Vol 59 (sup230) ◽

pp. 1-2 ◽

Cited By ~ 11

Author(s):

Mathias M Müller

Keyword(s):

Laboratory Data ◽

Current Status ◽

Future Trends ◽

Clinical Value ◽

Roche Diagnostics

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Comparison of modified Jaffe's kinetic method and enzymatic method of serum creatinine estimation in paediatric patients

Clinica Chimica Acta ◽

10.1016/j.cca.2019.03.1003 ◽

2019 ◽

Vol 493 ◽

pp. S475

Author(s):

C. Oliva ◽

M.d.C. Salgado ◽

L. Altimira ◽

A. Valls ◽

J. Velasco

Keyword(s):

Serum Creatinine ◽

Kinetic Method ◽

Enzymatic Method ◽

Paediatric Patients

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Recent Advances in the studies of Reaction Rates relevant to Interstellar Chemistry

Symposium - International Astronomical Union ◽

10.1017/s0074180900153707 ◽

1987 ◽

Vol 120 ◽

pp. 1-18

Author(s):

Nigel G. Adams ◽

David Smith

Keyword(s):

Laboratory Data ◽

Dissociative Recombination ◽

Reaction Rates ◽

Rate Coefficients ◽

Current Status ◽

Laboratory Measurements ◽

Interstellar Molecules ◽

Interstellar Clouds ◽

Radiative Association ◽

Molecular Synthesis

The current status of laboratory measurements of the rate coefficients for ionic reactions involved in interstellar molecular synthesis is discussed and the experimental techniques used to acquire such data are briefly described. Examples are given of laboratory data which are being obtained at temperatures close to those of interstellar clouds. Particular attention is given to the results of recent theoretical and experimental work which show that the rate coefficients for the binary reactions of ions with polar molecules at low temperatures are much larger than previously assumed. It is shown how these new developments in experiment and theory are reconciling the differences between predicted and observed abundances for some interstellar molecules. Also briefly discussed are: - the phenomenon of isotope exchange in ion/neutral reactions which explains the apparent enrichment of heavy isotopes in some interstellar molecules, the role of atoms in molecular synthesis, some studies of ion/neutral reactions pertaining to shocked regions of interstellar clouds, ternary association reactions and the analogous radiative association reactions, and recent new laboratory measurements of dissociative recombination coefficients. Finally, some guidance is offered in the proper choice of critical kinetic data for use in interstellar chemical modelling and some further requirements and likely future developments are mentioned.

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Outpatient Treatment with Intravenous Antimicrobial Therapy in Patients with Febrile Neutropenia and Hematologic Malignancies

Blood ◽

10.1182/blood.v112.11.4717.4717 ◽

2008 ◽

Vol 112 (11) ◽

pp. 4717-4717

Author(s):

Marcelo Bellesso ◽

Luis Fernando Pracchia ◽

Lucia Dias ◽

Dalton Chamone ◽

Pedro Dorlhiac-Llacer

Keyword(s):

High Risk ◽

Febrile Neutropenia ◽

Serum Creatinine ◽

Pulse Oximetry ◽

Antimicrobial Therapy ◽

Outpatient Treatment ◽

Laboratory Data ◽

Hematologic Malignancies ◽

Initial Therapy ◽

Low Risk

Abstract OBJECTIVES: The purpose of this study is to evaluate outcomes like success of the initial therapy; failure of outpatient treatment and death in outpatient treatment with intravenous antimicrobial therapy in patients with febrile neutropenia (FN) and hematologic malignancies. In addition, it was compared clinics, laboratory data and Multinational Association for Supportive Care of Cancer index (MASCC) with failure of outpatient treatment and death. PATIENTS AND METHODS: In a retrospective study we evaluated FN following chemotherapy events that were treated initially with Cefepime, with or without Teicoplanin. RESULTS: Of the 178 FN episodes in 128 patients, it was observed success of initial therapy in 63.5% events, failure of outpatient treatment in 20.8% and death in 6.2%. In multivariate analysis, significant risks of failure of outpatient treatment were smoking (OR: 3.14, IC: 1.14 – 8.66, p=0.027) and serum creatinine > 1.2mg/dL (OR: 7.97, IC: 2.19 – 28.95, p = 0.002). About death, the risk was pulse oximetry < 95% (OR: 5.8, IC: 1.50 – 22.56, p = 0.011). Analyzing MASCC index, 165 events were classified as low risk and 13 as high-risk. Failure of outpatient treatment were reported in connection with 7 (53.8%) high-risk episodes and 30 (18.2%) low-risk, p=0.006. In addition, death in 7 (4.2%) lowrisk and 4 (30.8%) high-risk events, p=0.004. CONCLUSIONS: The outpatient treatment with intravenous antibiotic was satisfactory. The risks: smoking, serum creatinine elevated and pulse oximetry should be considered in FN evaluation. It was validated MASCC index in Brazilian population.

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