Evaluation of the antibacterial influence of silver nanoparticles against fish pathogenic bacterial isolates and their toxicity against common carp fish

Abstract BackgroundCurrently, nanotechnology and nanoparticles have been quickly emerged and have gained the attention of scientists due to its massive applications in environmental sectors. However, these environmental applications of silver nanoparticles potentially cause serious effects on terrestrial and aquatic organisms. In the current study, freshwater fish C. carpio were exposed to blood mediated AgNPs for toxicity, mortality, bioaccumulation, and histological alterations. Silver nanoparticles were fabricated using animal blood serum and their toxic effect was studied against common carp fish at different concentrations level (0.03, 0.06, and 0.09 mg/L).ResultsThe findings have revealed a little effect of blood induced silver nanoparticles (B-AgNPs) on fish behavior at the highest concentration (0.09 mg/L). However, bioaccumulation of B-AgNPs was reported in different organs of fish. Maximum bioaccumulation of B-AgNPs was reported in the liver, followed by intestine, gills, and muscles. Furthermore, the findings have showed that the B-AgNPs bioaccumulation led to histopathological alterations including damage structure of gills tissue and caused necrosis. It is summarized that histopathological alteration in gills and intestine mostly occurred at the highest concentration of B-AgNPs (0.09 mg/L). ConclusionThis study provides evidence of the AgNPs influence on aquatic life; however, further systematic studies are crucial to access the effects of AgNPs on aquatic life.

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Characterization Of Phyto-Synthesized Silver Nanoparticles And Their Antimicrobial Activity Against Diabetic Foot Ulcer Bacterial Isolates

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs/lpr.2021.11.6.l88-96 ◽

2021 ◽

Vol 11 (6) ◽

pp. 88-96

Author(s):

Chhajed Shweta ◽

Arora Asha

Keyword(s):

Antimicrobial Activity ◽

Silver Nanoparticles ◽

Diabetic Foot ◽

Foot Ulcer ◽

Diabetic Foot Ulcer ◽

Lower Limbs ◽

Diffusion Method ◽

Foot Ulcers ◽

Diabetic Foot Ulcers ◽

Bacterial Isolates

Diabetic Foot Ulcer (DFU) is one of the major complications of Diabetes. Patients with Diabetic Foot ulcers have a high susceptibility to microbial infections and are the leading cause of hospitalization and amputation of lower limbs. In the era of increased prevalence of bacterial resistance and outbreak of resistant infectious diseases, it is very essential to develop effective therapeutic strategies towards multi-drug resistant pathogens. The antimicrobial properties of silver nanoparticles have been well studied, therefore their use in biomedicine and pharmacology is a trend. Herein we present the use of Phyto-mediated synthesized AgNPs for the treatment of diabetic foot ulcers by topical administration. The nanoparticles were synthesized by reducing silver nitrate using Terminalia chebula fruit extract. The nanoparticles were analyzed and characterized using UV-Visible Spectrophotometer, FTIR, XRD, SEM with EDAX, TEM, and DLS. The synthesized silver nanoparticles were assayed for antimicrobial activity against five Diabetic Foot Ulcer bacterial isolates i.e. Escherichia coli, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Streptococcus aureus, and Bacillus subtilis. The bactericidal property of synthesized nanoparticles was analyzed by the Agar well diffusion method, which revealed the remarkable antimicrobial effects against all the selective pathogenic bacterial isolates of Diabetic foot ulcers in the present study. These results constituted the basis for further studies on the use of plant-based silver nanoparticles for the treatment of Diabetic Foot ulcers from different origins

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