Optical tweezers combined with micro‐Raman investigation of alcohol‐induced changes on single, live red blood cells in blood plasma

2019 ◽  
Vol 50 (10) ◽  
pp. 1367-1374 ◽  
Author(s):  
Jijo Lukose ◽  
Mithun N ◽  
Ganesh Mohan ◽  
Shamee Shastry ◽  
Santhosh Chidangil
Keyword(s):  
Blood Plasma ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Induced Changes ◽  
In Blood Plasma

scholarly journals Oxidative stress in osteomyelitis and burns: Specific features of its role in development of pathology

2021 ◽  
Vol 8 (3) ◽  
pp. 4286-4293
Author(s):  
Andrew K. Martusevich ◽  
Valery I. Zagrekov ◽  
Ksenia L. Belyaeva ◽  
Alexander S. Pushkin ◽  
Anna G. Soloveva
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Blood Plasma ◽  
Red Blood Cells ◽  
Antioxidant System ◽  
Oxidative Metabolism ◽  
Blood Cells ◽  
Chronic Osteomyelitis ◽  
Wide Range ◽  
In Blood Plasma

Introduction: The purpose of this study was to evaluate and compare the features of the initiation and development of oxidative stress in patients with osteomyelitis and burns. Methods: We studied the oxidative metabolism of blood of 20 healthy subjects (controls), 15 patients with burns, and 18 patients with chronic osteomyelitis. All patients included in the second group had thermal burns of the I-II-III degree in trunk and limbs on an area of 31-80% of the body surface without thermal inhalation trauma. After standard sample preparation, a wide range of parameters of oxidative metabolism was determined in the blood. The intensity of free radical processes in blood plasma and red blood cells, and the total antioxidant activity was evaluated by Fe-induced biochemiluminescence. The concentration of malonic dialdehyde in blood plasma and red blood cells was determined. The level of diene and triene conjugates and Schiff bases was determined spectrophotometrically using reagent kits. The catalase and superoxide dismutase activities in the red blood cells of patients from each of the groups was also determined. Results: We showed that in osteomyelitis, which is a long-lasting process, changes in the balance of free radical generation and activity of the antioxidant system were compensatory and mostly related to changes in blood plasma. On the contrary, in burn victims, oxidative stress signs had a maladaptive character. They were seen in blood plasma and red blood cells, and accompanied by a pronounced depletion of enzyme antioxidant system reserves. Conclusion: Our study demonstrate the role of oxidative stress in patients with burns and chronic osteomyelitis, and demonstrate some specific features leading to formation of disease pathology. Such features of oxidative stress may be useful in future design of new approaches to correct the pathology of diseases.

scholarly journals Carbonyl Stress in Red Blood Cells and Hemoglobin

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 253
Author(s):  
Olga V. Kosmachevskaya ◽  
Natalia N. Novikova ◽  
Alexey F. Topunov
Keyword(s):  
Blood Plasma ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Ketone Bodies ◽  
Physiological State ◽  
Vascular Occlusion ◽  
Carbonyl Stress ◽  
First Case ◽  
Functional Features ◽  
In Blood Plasma

The paper overviews the peculiarities of carbonyl stress in nucleus-free mammal red blood cells (RBCs). Some functional features of RBCs make them exceptionally susceptible to reactive carbonyl compounds (RCC) from both blood plasma and the intracellular environment. In the first case, these compounds arise from the increased concentrations of glucose or ketone bodies in blood plasma, and in the second—from a misbalance in the glycolysis regulation. RBCs are normally exposed to RCC—methylglyoxal (MG), triglycerides—in blood plasma of diabetes patients. MG modifies lipoproteins and membrane proteins of RBCs and endothelial cells both on its own and with reactive oxygen species (ROS). Together, these phenomena may lead to arterial hypertension, atherosclerosis, hemolytic anemia, vascular occlusion, local ischemia, and hypercoagulation phenotype formation. ROS, reactive nitrogen species (RNS), and RCC might also damage hemoglobin (Hb), the most common protein in the RBC cytoplasm. It was Hb with which non-enzymatic glycation was first shown in living systems under physiological conditions. Glycated HbA1c is used as a very reliable and useful diagnostic marker. Studying the impacts of MG, ROS, and RNS on the physiological state of RBCs and Hb is of undisputed importance for basic and applied science.

Probing the Red Blood Cells Aggregating Force With Optical Tweezers

2016 ◽  
Vol 22 (3) ◽  
pp. 365-370 ◽  
Author(s):  
Kisung Lee ◽  
Anna V. Danilina ◽  
Matti Kinnunen ◽  
Alexander V. Priezzhev ◽  
Igor Meglinski
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells

Red Blood Cells in Patients with Arrhythmias Using Optical Tweezers Raman Spectroscopy

2011 ◽  
Vol 48 (2) ◽  
pp. 021701
Author(s):  
吴智辉 Wu Zhihui ◽  
莫华 Mo Hua ◽  
黄代政 Huang Daizheng ◽  
陈朝旺 Chen Chaowang
Keyword(s):  
Raman Spectroscopy ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells

Measurement of interaction forces between red blood cells in aggregates by optical tweezers

2012 ◽  
Vol 42 (6) ◽  
pp. 500-504 ◽  
Author(s):  
A Yu Maklygin ◽  
A V Priezzhev ◽  
A Karmenian ◽  
Sergei Yu Nikitin ◽  
I S Obolenskii ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells ◽  
Interaction Forces

Mechanical Properties of Stored Red Blood Cells Using Optical Tweezers

Blood ◽  
1998 ◽  
Vol 92 (8) ◽  
pp. 2975-2977 ◽  
Author(s):  
R.R. Huruta ◽  
M.L. Barjas-Castro ◽  
S.T.O. Saad ◽  
F.F. Costa ◽  
A. Fontes ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Blood Cells

scholarly journals Monitoring the side effects with DSC caused by cyclophosphamide treatment

2019 ◽  
Vol 142 (2) ◽  
pp. 765-770
Author(s):  
Péter Farkas ◽  
Franciska Könczöl ◽  
Dénes Lőrinczy
Keyword(s):  
Side Effects ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Peripheral Nerves ◽  
Chemotherapeutic Agents ◽  
Cyclophosphamide Treatment ◽  
Micro Dsc ◽  
Induced Changes ◽  
First Time ◽  
Dose Dependent

AbstractPolyneuropathy is defined as a simultaneous malfunction of several peripheral nerves, which could be a side effect of a cancer therapy (using cyclophosphamide) as well. In the daily use, it is very important to know the kinetics and metabolism of anticancer drugs because this way we can estimate their undesirable consequences to avoid the unwanted complications. Nowadays, the application of DSC in diagnosis of different diseases using blood compounds is increasing; therefore, the purpose of recent study was to introduce at the first time the DSC in the detection of cyclophosphamide-induced changes in plasma and red blood cells in case of an experimental animal model. Guinea pigs (Cavia porcellus, n = 60, in 12 different groups) underwent to cyclophosphamide treatment administrated intraperitoneally with the dose comparable to the human dosage. At the end of treatments, the animals were euthanized, and the experimental samples (plasma end red blood cells) were analyzed by a SETARAM Micro DSC-II calorimeter. The denaturation temperatures and the calorimetric enthalpies were calculated from the heat flow curves. Our results exhibited a dose-dependent difference between thermal parameters of untreated and treated samples, demonstrating that DSC is applicable in this field too. After deconvolution of DSC scans, the changes could be assigned to the attacked compounds. Recently published papers call the attention for this technique more frequently in the medical diagnosis because this way from small amount of sample very quickly and economically possible to detect and predict the expected and unwanted side effects of chemotherapeutic agents.

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