Phytic acid content may affect starch digestibility and glycemic index value of rice (
            
              Oryza sativa
            
            L.)

Lactic acid has known as a one of compounds to cause cellular harm in waterlogged tissue through the process of cytoplasmic acidosis. The effects of lactic acid on α-amylase activity and phytic acid content using an assay for high phosphate in germination stage of rice were evaluated. It is showed that lactic acid inhibited rice germination at every treated dose. The reduction of α-amylase content attributed to lactic acid at 24 h after germination of rice seeds was observed. The analysis of phosphate concentration at 48 h after treatment noted that although the content of phytic acid was reduced in Japonica varieties, but in contrast its amount was enhanced in Indica cultivars. The findings highlighted the positive effects of lactic acid on α-amylase activity and phytic acid content and suggested that this compound may play a potent role as a germinated regulator in rice.

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Novel allelic variant of Lpa1 gene associated with a significant reduction in seed phytic acid content in rice (Oryza sativa L.)

10.1101/493981 ◽

2018 ◽

Author(s):

D.S. Kishor ◽

Choonseok Lee ◽

Dongryung Lee ◽

Jelli Venkatesh ◽

Jeonghwan Seo ◽

...

Keyword(s):

Enzyme Activity ◽

Oryza Sativa ◽

Phytic Acid ◽

Oryza Sativa L ◽

Organic Phosphorus ◽

Brown Rice ◽

Chemical Mutagenesis ◽

Coding Region ◽

Entry Site ◽

Phytic Acid Content

In plants, myo-inositol-1,2,3,4,5,6-hexakisphosphate (InsP6), also known as phytic acid (PA), is a major component of organic phosphorus (P), and accounts for up to 85% of the total P in seeds. In rice (Oryza sativa L.), PA mainly accumulates in rice bran, and chelates mineral cations, resulting in mineral deficiencies among brown rice consumers. Therefore, considerable efforts have been focused on the development of low PA (LPA) rice cultivars. In this study, we performed genetic and molecular analyses of OsLpa1, a major PA biosynthesis gene, in Sanggol, a low PA mutant variety developed via chemical mutagenesis of Ilpum rice cultivar. Genetic segregation and sequencing analyses revealed that a recessive allele, lpa1-3, at the OsLpa1 locus (Os02g0819400) was responsible for a significant reduction in seed PA content in Sanggol. The lpa1-3 gene harboured a point mutation (C623T) in the fourth exon of the predicted coding region, resulting in threonine (Thr) to isoleucine (Ile) amino acid substitution at position 208 (Thr208Ile). Three-dimensional analysis of Lpa1 protein structure indicated that myo-inositol 3-monophosphate [Ins(3)P1] kinase binds to the active site of Lpa1, with ATP as a cofactor for catalysis. Furthermore, the presence of Thr208 in the loop adjacent to the entry site of the binding pocket suggests that Thr208Ile substitution is involved in regulating enzyme activity via phosphorylation. Therefore, we propose that Thr208Ile substitution in lpa1-3 reduces Lpa1 enzyme activity in Sanggol, resulting in reduced PA biosynthesis.

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Effect of Fermentation Methods on Phytic acid Content in Wheat Flour Bread

Basrah Journal of Agricultural Sciences ◽

10.33762/bagrs.2014.112578 ◽

2014 ◽

Vol 27 (1) ◽

pp. 128-142

Author(s):

Ali A. Sahi ◽

Ali H. Abdul-Kareem ◽

Basim A. Jaber

Keyword(s):

Phytic Acid ◽

Wheat Flour ◽

Acid Content ◽

Phytic Acid Content

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Rabadi fermentation of wheat: Changes in phytic acid content andin vitro digestibility

Plant Foods for Human Nutrition ◽

10.1007/bf02196463 ◽

1992 ◽

Vol 42 (2) ◽

pp. 109-116 ◽

Cited By ~ 17

Author(s):

Manju Gupta ◽

Neelam Khetarpaul ◽

B. M. Chauhan

Keyword(s):

Phytic Acid ◽

Acid Content ◽

Phytic Acid Content

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Metabolite profiling reveals the metabolic features of the progenies resulting from the low phytic acid rice (Oryza sativa L.) mutant

Journal of Cereal Science ◽

10.1016/j.jcs.2021.103251 ◽

2021 ◽

pp. 103251

Author(s):

Chenguang Zhou ◽

Yaojie Zhou ◽

Yuanyuan Tan ◽

Qingyao Shu ◽

Youfa Li ◽

...

Keyword(s):

Oryza Sativa ◽

Phytic Acid ◽

Metabolite Profiling ◽

Oryza Sativa L ◽

Low Phytic Acid

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Lowering of phytic acid content by enhancement of phytase and acid phosphatase activities during sunflower germination

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132010000400028 ◽

2010 ◽

Vol 53 (4) ◽

pp. 975-980 ◽

Cited By ~ 2

Author(s):

Juliana da Silva Agostini ◽

Rosicler Balduíno Nogueira ◽

Elza Iouko Ida

Keyword(s):

Acid Phosphatase ◽

Phytic Acid ◽

Acid Content ◽

Nutritional Value ◽

Phytase Activity ◽

Phytic Acid Content ◽

Phosphatase Activities ◽

Hybrid Sunflower

The objective of this work was to investigate the germination of hybrid sunflowers BRS191 and C11 as a means of lowering phytic acid (PA) content by enhancing the activity of endogenous phytase and acid phosphatase. The concentration of PA in hybrid sunflower achenes varied from 2.16 to 2.83g/100g of sample (p < 0.05). The phytase and acid phosphatase activities of sunflowers BRS191 and C11 were the highest on the 4th and 5th days of germination, respectively, with the release of the phosphorus. These results indicated that hybrid sunflower PA reduced and enhance phytase activity at distinct germination periods, which could open up the possibility of applying these enzymes in the control of PA content in cereals, thus improving their nutritional value.

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