Antioxidant activity screening and chemical constituents of the essential oil from rosemary by ultra‐fast GC electronic nose coupled with chemical methodology

2020 ◽  
Vol 100 (8) ◽  
pp. 3481-3487 ◽  
Author(s):  
Ji‐Yu Nie ◽  
Rong Li ◽  
Zi‐Tao Jiang ◽  
Ying Wang ◽  
Jin Tan ◽  
...  
2012 ◽  
Vol 7 (7) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Sukanya Keawsa-ard ◽  
Boonsom Liawruangrath ◽  
Saisunee Liawruangrath ◽  
Aphiwat Teerawutgulrag ◽  
Stephen G. Pyne

The essential oil of the leaves Solanum spirale Roxb. was isolated by hydrodistillation and analyzed for the first time using GC and GC-MS. Thirty-nine constituents were identified, constituting 73.36% of the total chromatographical oil components. ( E)-Phytol (48.10%), n-hexadecanoic acid (7.34%), β-selinene (3.67%), α-selinene (2.74%), octadecanoic acid (2.12%) and hexahydrofarnesyl acetone (2.00%) were the major components of this oil. The antioxidant activity of the essential oil was evaluated by using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging assay. The oil exhibited week antioxidant activity with an IC50 of 41.89 mg/mL. The essential oil showed significant antibacterial activity against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus with MIC values of 43.0 μg/mL and 21.5 μg/mL, respectively. It also showed significant cytotoxicity against KB (oral cancer), MCF-7 (breast cancer) and NCI-H187 (small cell lung cancer) with the IC50 values of 26.42, 19.69, and 24.02 μg/mL, respectively.


2015 ◽  
Vol 11 (43) ◽  
pp. 615 ◽  
Author(s):  
JacksonRoberto Guedes da Silva Almeida ◽  
Camilade Souza Araújo ◽  
AnaPaula de Oliveira ◽  
RafaelyNascimento Lima ◽  
PériclesBarreto Alves ◽  
...  

2019 ◽  
Vol 102 (5) ◽  
pp. 1448-1454
Author(s):  
Ying Zhao ◽  
Yang-Yang Fan ◽  
Wen-Gang Yu ◽  
Jian Wang ◽  
Wenju Lu ◽  
...  

Abstract Background: The essential oil content of the water lily is extremely low; thus, finding a new method that can extract essential oil from water lilies with a high extraction rate and no residual organic solvents is essential. Objective: The optimal processing conditions for the ultrasound-enhanced subcritical fluid extraction of essential oil from Nymphaea alba var (red water lily) and the antioxidant activity of the essential oil in vitro are investigated to provide theoretical bases for identification and development. Methods: Single-factor experiments and orthogonal designs are performed to determine the effects of extraction conditions on essential oil yields. The chemical composition of essential oil is analyzed using GC–MS. Results: The optimum extraction parameters are established as follows: extraction temperature, 35°C; extraction time, 30 min/time for four times; ratio of material to liquid, 1:3; ultrasound power, 250 W/L; and ultrasonic frequency, 20 kHz. The extraction rate of essential oil is 0.315% under these conditions. Eleven components comprise more than 1% content. The main chemical constituents are 8-hexadecyne (31.04%) and 2,6,10-trimethyl-tetradecane (3.95%). The essential oil from N. alba var has an antioxidant activity in vitro; however, its antioxidant activity is weaker than that of butylated hydroxytoluene. Conclusions: Subcritical fluid is suitable for the extraction of essential oil from N. alba var, and the essential oil has a good antioxidant activity. Highlights: The essential oil content of N. alba var is 0.315%. Forty-seven chemical constituents are identified and isolated from N. alba var and analyzed by GC–MS.


Author(s):  
Sanjay Kumar ◽  
Mohammad S Javed ◽  
Pawan Kumar ◽  
Rishendra Kumar

Objectives: The aim of our study was to evaluate the phytogenic chemical compounds and assess their antibacterial and antioxidant activity of essential oil of Ajuga parviflora Benth. growing in the Himalayan region. Methods: In the present study, the phytochemical constituents of essential oil were isolated by steam distillation and screened by gas chromatography (GC) and GC–mass spectrometry (GC-MS) analysis from A. parviflora is rich in oxygenated monoterpenoids and sesquiterpenoids. The essential oil was further evaluated for their antibacterial by well-diffusion method and antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay at various concentrations. Results: The major chemical constituents were α-cadinol (21.36%), α-muurolol (14.2%), cubebol (12.76%), germacrene D-4-ol (9.6%), germacrene D (4.32%), farnesyl acetate (3.58%), longifolol acetate (2.76%), and β-bourbonene (2.35 %) whereas monoterpenoids were minor constituents. The zone of inhibition (ZOI) shown by essential oil against test bacterial strains at concentration of 500 μg/ml to Gram-negative bacteria Pseudomonas aeruginosa (5.6 mm), Escherichia coli (5 mm), Salmonella typhimurium (8.6 mm), and Proteus vulgaris (10.7 mm) and Gram-positive bacteria Bacillus subtilis (8.3 mm). At the same time, ZOI of standard antibiotic ciprofloxacin against test bacterial strains was P. aeruginosa (9.6 mm), E. coli (14.3mm), S. typhimurium (19.3 mm), P. vulgaris (17.8 mm), and B. subtilis (20.6 mm), respectively. The free radical scavenging property of A. parviflora employed by in vitro assay methods like scavenging activity of DPPH was 81% at 500 μg/ml concentrations, respectively. Conclusion: Our study showed that α-cadinol, α-muurolol, and cubebol as the major components in this oil which was absent in previous findings of A. parviflora and essential oil had potent antibacterial and antioxidant activity, respectively.


2011 ◽  
Vol 125 (2) ◽  
pp. 456-463 ◽  
Author(s):  
Ling-Bin Zeng ◽  
Zhong-Rong Zhang ◽  
Zhu-Hua Luo ◽  
Ji-Xiao Zhu

2006 ◽  
Vol 6 (6) ◽  
pp. 1005-1010 ◽  
Author(s):  
Asmah Rahmat . ◽  
Susi Edrini . ◽  
Patimah Ismail . ◽  
Taufiq Yap Yun Hin . ◽  
Mohd Fadzelly Abu Ba .

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800
Author(s):  
Chakkrapat Singtothong ◽  
Michel J. Gagnon ◽  
Jean Legault

The aim of this study was to investigate the chemical composition and bioactivity of Amomum biflorum Jack harvested in the region of Petchaboon, Thailand. The essential oil of the fresh whole plant obtained by hydrodistillation was analyzed by gas chromatography (GC) (Kovats index) and gas chromatography coupled with mass spectrometry (GC/MS). The average yield of essential oil of A. biflorum was 0.21 ± 0.05 % (w/w). The major chemical constituents were camphor (17.6 %), α-bisabolol (16.0 %), camphene (8.2 %) and α-humulene (5.1 %). The essential oil was active against Staphylococcus aureus (MIC: 30 μ/mL) and had an antioxidant activity with an ORAC index of 23 ± 5 μmol Trolox/mg.


Sign in / Sign up

Export Citation Format

Share Document