Screening of radical scavenging activity and chemical constituents of the essential oil from star anise by ultra‐fast GC electronic nose coupled with DPPH, OH, and ABTS assays

Author(s):  
Ji‐Yu Nie ◽  
Yi Zhang ◽  
Rong Li ◽  
Zi‐Tao Jiang ◽  
Ying Wang ◽  
...  
Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2342
Author(s):  
Nikolaos Nenadis ◽  
Maria Papapostolou ◽  
Maria Z. Tsimidou

The present study examined the radical scavenging potential of the two benzene derivatives found in the bay laurel essential oil (EO), namely methyl eugenol (MEug) and eugenol (Eug), theoretically and experimentally to make suggestions on their contribution to the EO preservative activity through such a mechanism. Calculation of appropriate molecular indices widely used to characterize chain-breaking antioxidants was carried out in the gas and liquid phases (n-hexane, n-octanol, methanol, water). Experimental evidence was based on the DPPH• scavenging assay applied to pure compounds and a set of bay laurel EOs chemically characterized with GC-MS/FID. Theoretical calculations suggested that the preservative properties of both compounds could be exerted through a radical scavenging mechanism via hydrogen atom donation. Eug was predicted to be of superior efficiency in line with experimental findings. Pearson correlation and partial least square regression analyses of the EO antioxidant activity values vs. % composition of individual volatiles indicated the positive contribution of both compounds to the radical scavenging activity of bay laurel EOs. Eug, despite its low content in bay laurel EOs, was found to influence the most the radical scavenging activity of the latter.


2014 ◽  
Vol 66 (1) ◽  
pp. 401-413 ◽  
Author(s):  
Ksenija Mileski ◽  
Ana Dzamic ◽  
Ana Ciric ◽  
Slavica Grujic ◽  
M. Ristic ◽  
...  

This study was undertaken to determine the antioxidant and antimicrobial effect of essential oil and extracts of Echinophora sibthorpiana Guss. (fam. Apiaceae) collected in Macedonia. The chemical composition of E. sibthorpiana essential oil was characterized by the presence of methyl eugenol (60.40%), p-cymene (11.18%) and ?-phellandrene (10.23%). The free radical scavenging activity of extracts and essential oil was evaluated by DPPH and ABTS assays. The aqueous extract of aerial parts exhibited the strongest scavenging activity (IC50=1.67 mg/ml); results of the ABTS test showed that the most effective was the ethanol extract of aerial parts (1.11 mg vit. C/g). The essential oil showed stronger antioxidant activity compared to hydroxyanisole, ascorbic acid and quercetin that were used in the DPPH and ABTS tests, respectively. The total phenolic and flavonoid concentrations in the extracts ranged between 38.65-60.72 mg GA/g, and 3.15-19.00 mg Qu/g, respectively. The antimicrobial properties of the extracts and essential oil were investigated using a micro-well dilution technique against human pathogenic strains. The results were comparable with the effects of the positive controls, streptomycin and fluconazole. These findings indicate that E. sibthorpiana extracts and oil can be used in preventive treatments and as an alternative for synthetic preservatives.


2018 ◽  
Vol 13 (7) ◽  
pp. 1934578X1801300
Author(s):  
Gislaine Aurelie Kemegne ◽  
Maximilienne Ascension Nyegue ◽  
Sylvain Leroy Sado Kamdem ◽  
François-Xavier Etoa ◽  
Chantal Menut

Ethnobotanic surveys have revealed the use of Mangifera indica L. (Anacardiaceae) bark for the treatment of infectious diarrhea. The essential oil of M. indica bark is described for the first time for its chemical composition, radical scavenging activity (DPPH method) and antimicrobial properties. The total phenols content of its water and ethanol bark extracts as well as their radical scavenging and antimicrobial properties were also evaluated. Four commercial plant extracts were also studied for a comparison purpose. The antimicrobial activities were measured for all samples against three Gram (-): Escherichia coli, Salmonella enteritidis, Shigella and two Gram (+): Staphylococcus aureus and Bacillus cereus bacteria. The M. indica bark essential oil was characterized by the association of two major sesquiterpenes: ( E)-β-caryophyllene (60.3%) and α-humulene (36.7%). It presented the lowest ratio of concentration to inhibition zone diameter on all the strains. The aqueous and ethanol extracts of M. indica bark were characterized by high contents of total phenols compounds and high radical scavenging activity compared to the essential oil. Finally, the interesting combination of the antibacterial and antiradical activities of the aqueous M. indica bark extract justifies the traditional use of this plant part in decoction form for the treatment of diarrheal infections.


2013 ◽  
Vol 3 (1) ◽  
pp. 77-80
Author(s):  
Jeanne Eliane R. Lantovololona ◽  
Ferdinand Mouafo Talontsi ◽  
Lygie Randriambola ◽  
Hartmut Laatsch

2012 ◽  
Vol 506 ◽  
pp. 393-396 ◽  
Author(s):  
A. Thakam ◽  
N. Saewan

Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract > DCM extract > n-butanol extract > aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P<0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction


2008 ◽  
Vol 3 (4) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Elena Stashenko ◽  
Carlos Ruiz ◽  
Amner Muñoz ◽  
Martha Castañeda ◽  
Jairo Martínez

Microwave-assisted hydrodistillation, simultaneous distillation-solvent extraction, and supercritical fluid extraction, were used to isolate secondary metabolites from two Lippia origanoides chemotypes growing wild in Colombia. Compound identification was based on chromatographic and spectroscopic criteria. The main components identified in the essential oil of one chemotype were carvacrol (44.4 – 51.8%) and p-cymene (8.8 – 10.1%). Those of the other chemotype were p-cymene (11.3 – 15.7%) and 1,8-cineole (6.8 – 10.9%). The last one constitutes a new chemotype, characterized by its low thymol and carvacrol contents and very low antioxidant activity. The radical scavenging activity of the essential oil of the first chemotype was higher than that of butylated hydroxytoluene (BHT) and similar to that of α-tocopherol.


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