scholarly journals Generation of an in vitro model of the outer annulus fibrosus‐cartilage interface

JOR Spine ◽  
2020 ◽  
Vol 3 (2) ◽  
Author(s):  
Jasmine E. Chong ◽  
J. Paul Santerre ◽  
Rita A. Kandel
Keyword(s):  
Annulus Fibrosus ◽  
In Vitro Model ◽  
Outer Annulus ◽  
Vitro Model ◽  
Outer Annulus Fibrosus

Dynamic Hydrostatic Pressurization Differentially Regulates Extracellular Matrix Elaboration by Bovine Inner and Outer Annulus Fibrosus Cells Seeded on 3-D Polymer Scaffolds

2007 ◽  
Author(s):  
Anna T. Reza ◽  
Steven B. Nicoll
Keyword(s):  
Extracellular Matrix ◽  
Mechanical Loading ◽  
Annulus Fibrosus ◽  
Type Ii Collagen ◽  
Culture Conditions ◽  
Phenotypic Properties ◽  
Outer Annulus ◽  
The Impact ◽  
Outer Annulus Fibrosus

Current surgical treatments for intervertebral disc (IVD) degeneration result in decreased mobility of the spine [1]. A tissue engineering approach may provide an alternative that restores both IVD structure and function. The IVD is comprised of three distinct regions: the outer annulus fibrosus (OA), inner annulus fibrosus (IA), and the nucleus pulposus (NP). Each of the cell populations within these regions possess unique phenotypic properties that are greatly influenced by environmental factors, such as the surrounding 3-D extracellular matrix (ECM) and mechanical loading (i.e., hydrostatic pressurization) [2]. As such, both the 3-D scaffold and in vitro culture conditions may have marked effects on the development of tissue-engineered IVD constructs. Although the influence of mechanical loading on IVD cells and explants has been investigated, no prior studies have examined the impact of hydrostatic pressurization on OA and IA cells in 3-D culture. Therefore, the objective of this study was to determine the effects of dynamic hydrostatic pressurization on OA and IA cells seeded on 3-D fibrous poly(glycolic acid)-poly(L-lactic acid) (PGA-PLLA) scaffolds. We hypothesized that the application of hydrostatic pressure would promote increased production of type II collagen and chondroitin sulfate proteoglycan in both OA- and IA-seeded constructs.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

Präsidentenposter: Regulation der Expression der β-Defensine hBD-2 und hBD-3 in einem in vitro Model der Candida Ösophagitis: NF-κB, MAPK/AP-1 und EGFR

2009 ◽  
Vol 47 (09) ◽  
Author(s):  
N Steubesand ◽  
K Kiehne ◽  
R Pahl ◽  
G Brunke ◽  
UR Fölsch ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Vitro Model

A new in vitro model for pre-transplantation diagnosis of frozen/thawed human ovarian tissue

2011 ◽  
Vol 71 (05) ◽  
Author(s):  
M Salama ◽  
K Winkler ◽  
KF Murach ◽  
S Hofer ◽  
L Wildt ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Ovarian Tissue ◽  
Human Ovarian Tissue ◽  
Vitro Model

A novel in vitro model for joint effects of alcohol and free fatty acids on hepatocellular steatosis and inflammation

2013 ◽  
Vol 51 (01) ◽  
Author(s):  
A Mahli ◽  
WE Thasler ◽  
M Müller ◽  
C Hellerbrand
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
In Vitro Model ◽  
Joint Effects ◽  
Vitro Model
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