Non‐targeted metabonomics to investigate the differences in the properties of ginseng and American ginseng based on rapid resolution liquid chromatography coupled with quadrupole‐time‐of‐flight mass spectrometry

2021 ◽  
Author(s):  
Mengmeng Li ◽  
Shengyan Hua ◽  
Xin Huang ◽  
Hao Yue ◽  
Changbao Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Time Of Flight ◽  
American Ginseng ◽  
Rapid Resolution ◽  
Flight Mass Spectrometry

Nano and rapid resolution liquid chromatography-electrospray ionization-time of flight mass spectrometry to identify and quantify phenolic compounds in olive oil

2010 ◽  
Vol 33 (14) ◽  
pp. 2069-2078 ◽  
Author(s):  
Rocío García-Villalba ◽  
Alegría Carrasco-Pancorbo ◽  
Gabriela Zurek ◽  
Marina Behrens ◽  
Carsten Bäßmann ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Olive Oil ◽  
Electrospray Ionization ◽  
Time Of Flight ◽  
Ionization Time ◽  
Rapid Resolution ◽  
Flight Mass Spectrometry

scholarly journals Ultra-Performance Liquid Chromatography and Time-of-Flight Mass Spectrometry Analysis of Ginsenoside Metabolites in Human Plasma

2011 ◽  
Vol 39 (06) ◽  
pp. 1161-1171 ◽  
Author(s):  
Chong-Zhi Wang ◽  
Karen E. Kim ◽  
Guang-Jian Du ◽  
Lian-Wen Qi ◽  
Xiao-Dong Wen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Intestinal Microbiota ◽  
Human Subjects ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
American Ginseng ◽  
Ginseng Root ◽  
Flight Mass Spectrometry

American ginseng is a commonly used herbal medicine in the United States. When ginseng is taken orally, its active components, ginsenosides, are reportedly biotransformed by intestinal microbiota. Previous pharmacokinetic evaluations of ginseng in humans have focused on its parent constituents. However, the metabolites, especially those transformed by intestinal microbiota, have not been carefully studied. We used an ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC/TOF-MS) method to determine 15 ginsenosides and/or metabolites and their bioavailability in humans. Six healthy human subjects received a single oral dose of 10 g of American ginseng root powder, after which samples of their blood were collected at 0, 2, 4, 7, 9 and 12 h for measurement of ginsenoside/metabolite levels in plasma. Ginsenosides Rb1, Rd, Rg2 and compound K (C-K) were detected in human plasma samples at different time points. The Rb1 concentration peak was 19.90 ± 5.43 ng/ml at 4 h. C-K was detected from 7 h to 12 h with 7.32 ± 1.35 ng/ml at 12 h. Since the last time point was at 12 h, C-K peak level was not observed. The areas under the concentration curves (AUC) from 0 to 12 h were 155.0 ± 19.5 ng⋅h/ml for Rb1 and 26.4 ± 6.4 ng⋅h/ml for C-K, respectively. The gradual decrease of Rb1 levels and the delayed increase in levels of C-K observed in human subjects supported previous reports that enteric microbiota played a key role in transforming Rb1 to C-K.

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