Identification and Analysis of Bacterial Contamination of Ultrasound Transducers and Multiuse Ultrasound Transmission Gel Bottle Tips Before and After the Aseptic Cleansing Technique

2020 ◽  
Vol 39 (10) ◽  
pp. 1957-1963 ◽  
Author(s):  
Kevin Mullins ◽  
Kevin Burnham ◽  
Erik K. Henricson ◽  
Stuart Cohen ◽  
James Fair ◽  
...  
2018 ◽  
Vol 33 (6) ◽  
pp. 602-606
Author(s):  
Roohollah Farhadloo ◽  
Jalil Goodarzi Far ◽  
Mohammad Reza Azadeh ◽  
Saeed Shams ◽  
Mohammad Parvaresh-Masoud

AbstractBackgroundThe contamination of the environment, ambulance equipment, and staff hands consequently are major factors which create nosocomial infections in emergency patients. The contamination of equipment and devices plays an important role in nosocomial infections.ObjectivesThe aim of this study was to determine the effectiveness of a disinfectant on the rate of microbial contamination of ambulances in Qom Emergency Medical Services (EMS), Qom, Iran.MethodsThis is a quasi-experimental study with a before-after design in order to determine microbial contaminations at the rear and front cabin of ambulances, as well as medical equipment being utilized in Qom EMS. Saya sept-HP-2% solution was used for disinfection. Bacteriological standard methods were used to identify the contaminations.ResultsThe contamination rates before and after use of disinfection solution were 52% and eight percent, respectively. Coagulase-negative staphylococci were the most commonly isolated bacterial agent from the equipment (53%). In all equipment, the contamination level has shown a significant reduction after applying disinfectant.Conclusions:In spite of the fact that the rate of infection from ambulance equipment is high, the results showed that the use of the suitable disinfectant had an effective role in the reduction of bacteria.FarhadlooR, Goodarzi FarJ, AzadehMR, ShamsS, Parvaresh-MasoudM.Evaluation of bacterial contamination on prehospital ambulances before and after disinfection. Prehosp Disaster Med. 2018;33(6):602–606.


2021 ◽  
Vol 15 ◽  
pp. 117863022110375
Author(s):  
Derebew Aynewa ◽  
Zemichael Gizaw ◽  
Aklilu Feleke Haile

Background: Meat safety is important for public health. As part of the meat chain abattoirs are required to give attention to meat hygiene and safety in order to minimize hazards. Therefore, the current study was conducted to evaluate the bacteriological quality of sheep carcasses, knowledge and hygienic practices of workers in a selected abattoir and to determine the effect level of 2.5% citric acid spray on total coliforms and aerobic bacteria load of raw sheep carcasses surfaces. Methods: A cross-sectional study design with structured questionnaire and observational checklists observation were used. A systematic random sampling technique was employed. A total of 50 sample swabs (25 swabs before citric acid spray and 25 after citric acid spray) were randomly taken from brisket, flank and rump of sheep’s carcasses. Swabs were moistened with buffered peptone water (BPW) and samples were taken by rubbing 100 cm2 (10 cm × 10 cm) area delineated by sterile aluminum template. In addition, we administered a structured questionnaire and an observational checklists to assess knowledge and hygienic practices of workers. Bacteriological quality of sheep carcasses were analyzed using the methods described by the US bacteriological analytical manual. Results: The mean count for aerobic bacteria of the sheep carcasses before and after citric acid spray were 7.2log10 CFU/ml and 6.4log10 CFU/ml, respectively. The test results also showed that 21 (84%) and 15 (60%) of the swab samples were positive before and after spraying citric acid, respectively. The mean counts for coliform bacterial of the sheep carcasses before and after citric acid spray were 3.5log10 CFU/ml and 2.9log10 CFU/ml, respectively. The mean total aerobic and coliform counts before and after citric acid spray were significantly different ( P < .05). Regarding the hygiene condition of workers, all the respondents reported that they always washed their hands with soap before and after entering the slaughtering room and 23 (53.5%) of the workers reported that they used hot water. Thirty-one (72.1%) of the workers reported that they do not used soap to wash hands after visiting toilet. Thirty-five (81.4%) of the production workers did not wear mouth mask while handling and distribute meat/carcass. On the other hand, all of the workers wore capes, gowns and boots at the time of the observation and only 18 (18.6%) of the production workers wore gloves at the time of the survey. Conclusion: The current study revealed that significant proportion of sheep carcasses were positive for total aerobic bacteria and total coliform. Moreover, the study also showed that spraying of sheep carcasses with 2.5% citric acid significantly reduced the total coliform and aerobic counts. However, we did not assessed how much spray results to this effect. Therefore, we recommended further studies to determine how much spray of 2.5% citric acid significantly reduce bacterial contamination of sheep carcasses. In addition, the abattoir has to follow the food hazard analysis critical control point (HACCP) system to minimize meat contamination during harvesting and processing. The abattoir has to also implement strict operation laws to improve hygiene conditions of the workers. In addition, the abattoir can minimize meat contamination using 2.5% citric acid as a decontaminant.


Transfusion ◽  
2009 ◽  
Vol 49 (10) ◽  
pp. 2152-2157 ◽  
Author(s):  
Masahiro Satake ◽  
Takako Mitani ◽  
Shinji Oikawa ◽  
Hideto Nagumo ◽  
Sayoko Sugiura ◽  
...  

2004 ◽  
Vol 25 (3) ◽  
pp. 192-197 ◽  
Author(s):  
Carmem Lúcia Pessoa-Silva ◽  
Sasi Dharan ◽  
Stéphane Hugonnet ◽  
Sylvie Touveneau ◽  
Klara Posfay-Barbe ◽  
...  

AbstractObjective:To evaluate the dynamics of bacterial contamination of healthcare workers' (HCWs) hands during neonatal care.Setting:The 20-bed neonatal unit of a large acute care teaching hospital in Geneva, Switzerland.Methods:Structured observation sessions were conducted. A sequence of care began when the HCW performed hand hygiene and ended when the activity changed or hand hygiene was performed again. Alcohol-based handrub was the standard procedure for hand hygiene. An imprint of the five fingertips of the dominant hand was obtained before and after hand hygiene and at the end of a sequence of care. Regression methods were used to model the final bacterial count according to the type and duration of care and the use of gloves.Results:One hundred forty-nine sequences of care were observed. Commensal skin flora comprised 72.4% of all culture-positive specimens (n = 360). Other microorganisms identified were Enterobacteriaceae (n = 55, 13.8%); Staphylococcus aureus (n = 10, 2.5%); and fungi (n = 7,1.8%). Skin contact, respiratory care, and diaper change were independently associated with an increased bacterial count; the use of gloves did not fully protect HCWs' hands from bacterial contamination.Conclusions:These data confirm that hands become progressively contaminated with commensal flora and potential pathogens during neonatal care, and identify activities at higher risk for hand contamination. They also reinforce the need for hand hygiene after a sequence of care, before starting a different task, and after glove removal.


2018 ◽  
Vol 51 ◽  
pp. 310-318
Author(s):  
Ye. M. Kryvokhyzha ◽  
O. M. Zhukorskiy ◽  
O. V. Nykyforuk ◽  
O. B. Lesyk

The goal of the work was the study of efficiencyof sanitary processing of milking and dairy equipment in private household farms and evaluation of ecological risks of use of different detergent-disinfectants. Research methods used. Comparative assessment of the effectiveness of sanitary processing of milking and dairy equipment in private household farms was conducted using commercially available in Ukraine detergent-disinfectants: domestic, alkaline – Hlorantoin (active ingredients –sodium carbonate, sulphonol, sodium tripolyphosphate and corrosion inhibitor) and foreign, neutral – Eco des, (cationic surfactants). Imported acid detergents, including: Eco cid, Hipracid and Acid XD and acid detergent TDS, developed by scientists of Ternopil Experimental Station IVM NAAS, were used. The sanitary-hygienic and ecological estimation for the use of differentdetergent- disinfectants was conducted in private household farms of Chernivtsi region. All the detergent-disinfectants were used in concentrations and at temperature according to the instructions for use. Cow’s udder before milking was sanitized with disposable wipes moistened with0,5% solution of Kenopur produced by "Cid Lines" company. Milking machines, bucketsfor milk and glass jars storing freshly drawn milk before transfer to procurement rural assembly point are subjected to sanitary processing. The sanitary processing of milking machines was conducted at once after completion of milking of cows in the automatic mode and dairy utensils (bucket for milk and glass jars) after pouring off milk was carried out by hand with the use of a brush. The scheme of sanitary processing of dairy utensils included next operations: previous rinsingof milking and dairy equipment from residue of milk with water at temperatures +35–45 ºС in an amount of 8 litres; processing with the investigated solution of detergent-disinfectants at temperatures +50–60 ºС during 2 minutes (in an amount of 10 litres); rinsing of milking and dairy equipment from the remnants of alkaline or neutral detergentswith water at temperatures +35–45 ºС in an amount of 8 litres. For milking machines additionally, it was conducted: processing with acid detergentsolution at temperatures +50–60 ºС during 2 minutes (in an amount of 10 litres) and rinsing of milking machines from the remnants of acid detergentswith waterat temperature of +35–45 °C in an amount of 8 litres. Bacterial sampling and samples of obtained milk were conducted before and after realization of sanitary processing of portable milking machines from the internal surface of milking rubber, milk collector to the hose, tank of milking machines. Bacterial sampling from the internal surface of bucket for milk and glass jars and also milk, obtained from glass jars, was conducted for the analysis of the sanitary state of dairy utensils. Determination of the number of bacterial colonies from swab of sample and milk was carried outby cup method. Sowing was cultivated in a thermostat at temperatures +30 °С during 72 hours. Basic conclusions of this study. Use of detergent-disinfectant Eco des for the sanitary processing of dairy utensils in the private household farms by hand way reduces bacterial contamination, on average by 99,8 %, compared with the level of bacterial contamination before processing. Efficiency of application of Eco des washigher by 9,3 % compared with Hlorantoin and allows to support dairy utensils in the proper sanitary state. Using such detergent-disinfectants as Eco des and TDS for the sanitary processing of milking machines is more effective compared with Hlorantoin and Acid XD and allows to reduce microbial contamination, on average, by 99,5 %, that provides an opportunity to get milk with the high microbiological indicators of quality. Analysis of ecological risks of realization of sanitary processing in 80 private household farms with a total of 100 cows for the use of detergent-disinfectant Eco des showed decreasing residuals of detergent-disinfectants incoming into the environment, including phosphates by 2956,5–4161,0 kg/year and sulphonol by 1095,0–1642,5 kg/year. Domestic acid detergent TDS provides excellent destruction of milk stone and doesn’t contain phosphoric acid. Its use decreases the amount of nitrogen incoming into the environment by 40% compared with imported detergentAcid XD. The use of detergent-disinfectants such as Eco des and TDS for sanitary processing of milking and dairy equipment in the private household farms reduces the probability of violation of natural biocenoses.


2021 ◽  
Author(s):  
Madline Gund ◽  
Gabor Boros ◽  
Matthias Hannig ◽  
Sigrid Thieme-Ruffing ◽  
Barbara Gärtner ◽  
...  

Abstract Background Microbial contamination of dental professionals’ facial protective equipment and skin from aerosols and droplets generated during dental treatment has not been studied in all aspects so far. Our aim was therefore to investigate and compare treatment-related bacterial contamination of the forehead skin and surgical mask of dental practitioners. Methods We analyzed samples from 67 consecutive aerosol-producing conservative-preventive dental treatments. Sterile nylon swabs were used to collect samples from the forehead skin before and after performing treatment. Contact samples were obtained from the used surgical face masks. Samples were cultivated on agar under aerobic and anaerobic conditions. Bacteria were classified by MALDI-TOF mass spectrometry. The frequency of detection of obligate and facultative oral bacteria, as well as an increase in bacterial abundance, were examined (bacterial scoring: 0: no growth on agar; 1: <102 colonies; 2: >102 colonies; 3: dense bacterial growth). Results Bacteria were detected in 95% of the skin swabs and 76% of mask samples. Median bacterial scores were 2 for the forehead skin samples before and after treatment, and 1 for samples obtained from masks. Obligate and facultative oral bacteria were more frequent (6% and 30%) in samples from forehead skin after treatment. Samples taken from the forehead skin after treatment showed increased scores for these bacteria (28%). Five percent of samples were positive for methicillin-sensitive Staphylococcus aureus (MSSA). Obligate anaerobes were present in 3% of samples. Conclusions Although exposed during treatment, the forehead skin was significantly less contaminated with obligate oral bacteria than expected based on the surgical face mask findings. Forehead skin samples showed an increase in bacteria in the after treatment, which can be attributed to aerosol-producing procedures. We hypothesize that the forehead’s physiological skin flora may offer some protection against contamination with other microorganisms.


2020 ◽  
Vol 49 (7) ◽  
pp. 1307-1314
Author(s):  
Justin M. Lavallée ◽  
Cindy Shmon ◽  
Hugues Beaufrère ◽  
Manuel Chirino‐Trejo ◽  
Kathleen Linn

Author(s):  
AISHWARYA HARIKRISHNAN ◽  
G. BHUVANESHWARI

Objective: The present study is to assess the effectiveness of chlorhexidinegluconate as a disinfectant for the stethoscope diaphragm, to survey the routine practices followed by health care professionals (HCPs) towards stethoscope disinfection and to ascertain their general awareness on the matter. Methods: Questionnaire-based study conducted to assess the degree of awareness and attitude of HCPs towards stethoscope disinfection procedures. 200 responses were obtained and analyzed. Randomized, blind trial comparing bacterial load of stethoscope post-exposure to chlorhexidinegluconate vs. ethyl alcohol. The swabs collected from stethoscope diaphragm before and after exposure to disinfectant, were cultured using spread plate technique on nutrient agar. CFU count was compared. Results: Out of 200, nearly all HCPs part of this study were aware that stethoscopes could be linked to transmission of healthcare-associated infections (HCAIs) but only 78.5% (157) were accustomed to stethoscope disinfection practices. Although only 13.5% (27/200) of participants believed that chlorhexidinegluconate was effective, this study found it to be highly efficacious against bacterial recontamination. Conclusion: We stress upon the importance of regular disinfection of the stethoscope after each use and encourage frequent cleaning of the device with chlorhexidinegluconate, which may reduce bacterial recontamination for a longer period.


Medicine ◽  
2021 ◽  
Vol 100 (13) ◽  
pp. e25285
Author(s):  
Gisele Alborghetti Nai ◽  
Denis Aloísio Lopes Medina ◽  
Cesar Alberto Talavera Martelli ◽  
Mayla Silva Cayres de Oliveira ◽  
Maria Júlia Schadeck Portelinha ◽  
...  

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