Preparation of Anti‐Nonspecific Adsorption Chitosan‐Based Bovine Serum Albumin Imprinted Polymers with Outstanding Adsorption Capacity and Selective Recognition Ability Based on Magnetic Microspheres

Bovine serum albumin is one of the major serum proteins; it plays an important role as a result of its functional and nutritional properties which have bioactive peptides. Adsorption method was used to separate protein, which involves hydroxyapatite, synthetic hydroxyapatite, and active babassu coal. Initially, characterization was carried out using the zeta potential of the adsorbents. Kinetic pseudo-first- and pseudo-second-order models were applied. For isotherms, equilibrium data studies were carried out using the Langmuir and Freundlich models, in addition to determining the efficiency of adsorptive process. The results of the zeta potential showed loads ranging from +6.9 to −42.8 mV. The kinetic data were better represented in the pseudo-second-order model with chemisorption characteristics. The adsorption capacity of the adsorbents decreased as pH increased, indicating that the electrostatic bonds and some functional groups of active babassu coal contributed to the reduction of adsorption, especially oxygen linked to carbon atoms. The value of pH 4.0 showed the best results of adsorption, being obtained as the maximum adsorption capacity (qm) and yield (%) (whereqm=87.95 mg g−1and 74.2%; 68.26 mg g−1and 68.6%; and 36.18 mg g−1, 37.4%) of hydroxyapatite, synthetic hydroxyapatite, and active babassu coal, respectively.

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Bovine Serum Albumin Adsorption onto Hydroxyapatite and Biphasic Calcium Phosphate Ceramic Granules

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.721.197 ◽

2016 ◽

Vol 721 ◽

pp. 197-201 ◽

Cited By ~ 2

Author(s):

Inga Jurgelane ◽

Armands Buss ◽

Dagnija Loca

Keyword(s):

Calcium Phosphate ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Adsorption Capacity ◽

Bovine Serum ◽

Biphasic Calcium Phosphate ◽

Calcium Phosphate Ceramic ◽

Beta Tricalcium Phosphate ◽

Albumin Adsorption ◽

Hydroxyapatite Formation

Calcium phosphate ceramics are one of the most studied biomaterials for bone substitution and regeneration. Bioactivity is one of the most important properties for these materials and it can be evaluated by adsorption of proteins and by hydroxyapatite formation on the surface in simulated body fluid (SBF). The aim of this work was to evaluate the bioactivity of ceramic granules with various hydroxyapatite (HAp) and beta tricalcium phosphate (β-TCP) ratios by adsorption of bovine serum albumin (BSA) in SBF and phosphate saline buffer (PBS) solution. The highest adsorption capacity in both solutions was observed for biphasic calcium phosphate sample with HAp/β-TCP ratio 50/50 but the lowest – for sample 10/90. The adsorption capacity of all samples was more than 2 times higher in SBF media than in PBS. The possibility of hydroxyapatite formation was evaluated by changes of Ca2+ concentration in SBF. After 5 days of incubation at 37oC all samples showed a decrease in Ca2+ concentration.

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Chitosan nanoparticle carrier based on surface molecularly imprinted polymers for the recognition and separation of proteins

RSC Advances ◽

10.1039/c5ra14088a ◽

2015 ◽

Vol 5 (128) ◽

pp. 106197-106205 ◽

Cited By ~ 7

Author(s):

Cenjin Zhang ◽

Yuzhi Wang ◽

Junxia Guo ◽

Yanjin Liu ◽

Yigang Zhou

Keyword(s):

Aqueous Solution ◽

Bovine Serum Albumin ◽

Serum Albumin ◽

Molecularly Imprinted Polymers ◽

Bovine Serum ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Modified Chitosan ◽

Chitosan Nanoparticle ◽

Separation Of Proteins

This paper discusses the construction of surface molecularly imprinted polymers (MIPs) based on modified chitosan (CS) nanoparticle carriers to recognize and separate bovine serum albumin (BSA, pI 4.9, MW 69.0 kDa) in aqueous solution.

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