Sperm storage influences the potential for spontaneous acrosome reaction of the sperm in the newt Cynops pyrrhogaster

2017 ◽  
Vol 84 (12) ◽  
pp. 1314-1322 ◽  
Author(s):  
Shinnosuke Kon ◽  
Tae Sato ◽  
Daisuke Endo ◽  
Tomoe Takahashi ◽  
Akio Takaku ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Sperm Storage ◽  
Cynops Pyrrhogaster

Identification of egg-jelly substances triggering sperm acrosome reaction in the newt,Cynops pyrrhogaster

2009 ◽  
Vol 76 (4) ◽  
pp. 399-406 ◽  
Author(s):  
Akihiko Watanabe ◽  
Keiko Fukutomi ◽  
Hideo Kubo ◽  
Manami Ohta ◽  
Eriko Takayama-Watanabe ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Cynops Pyrrhogaster ◽  
Egg Jelly ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Identification of the sperm motility-initiating substance in the newt, Cynops pyrrhogaster, and its possible relationship with the acrosome reaction during internal fertilization

2010 ◽  
Vol 54 (4) ◽  
pp. 591-597 ◽  
Author(s):  
Toshihiko Watanabe ◽  
Hideo Kubo ◽  
Shinya Takeshima ◽  
Mami Nakagawa ◽  
Manami Ohta ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Acrosome Reaction ◽  
Internal Fertilization ◽  
Cynops Pyrrhogaster

The activity for the induction of the sperm acrosome reaction localises in the outer layers and exists in the high-molecular-weight components of the egg-jelly of the newt, Cynops pyrrhogaster

Zygote ◽  
2002 ◽  
Vol 10 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Takayuki Sasaki ◽  
Saori Kamimura ◽  
Hiroyuki Takai ◽  
Akihiko Watanabe ◽  
Kazuo Otinake
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Acrosome Reaction ◽  
Dependent Manner ◽  
Strong Activity ◽  
Maclura Pomifera ◽  
Cynops Pyrrhogaster ◽  
Sds Page ◽  
Egg Jelly ◽  
Dose Dependent

Localisation of the acrosome reaction inducing activity in egg-jelly was examined in the newt, Cynops pyrrhogaster. The jelly has six layers: the J0, J1, J2, J3, J4 and st layers. Jelly was mechanically dissected and placed on a Millipore filter. When sperm were added from the outer surface side of the jelly, most of them exhibited the acrosome reaction after passing through the jelly. When egg-jelly was divided into four layers, strong activity for the induction of acrosome reaction was detected in the outer layers, J4+st. These findings suggest that the acrosome reaction is induced by a substance in the outer layers of the egg-jelly. Among jelly components separated by SDS-PAGE, a fraction of more than 500 kDa in molecular weight induced the acrosome reaction. Wheat germ agglutinin (WGA), Griffonia simplicifoliar agglutinin 1 (GS-1), Maclura pomifera agglutinin (MPA) and Arachis hypogaea agglutinin (PNA) inhibited the induction of the acrosome reaction by jelly extract, and WGA did so in a dose-dependent manner. Those lectins precipitated some molecules of over 500 kDa. These results suggest that the acrosome reaction is induced by the high molecular-weight components of egg-jelly in C. pyrrhogaster.

Contribution of different Ca2+ channels to the acrosome reaction-mediated initiation of sperm motility in the newt Cynops pyrrhogaster

Zygote ◽  
2013 ◽  
Vol 23 (3) ◽  
pp. 342-351 ◽  
Author(s):  
Eriko Takayama-Watanabe ◽  
Hiroto Ochiai ◽  
Shunpei Tanino ◽  
Akihiko Watanabe
Keyword(s):  
Sperm Motility ◽  
Acrosome Reaction ◽  
Emission Spectrometry ◽  
Cynops Pyrrhogaster ◽  
Reliable Source ◽  
Voltage Dependent ◽  
Egg Jelly ◽  
High Level ◽  
Sequential Gating ◽  
Ca2 Channels

SummaryInitiation of sperm motility in urodeles, which is induced by a sperm motility-initiating substance (SMIS) in the sequestered granules on the surface of egg jelly, is mediated by the acrosome reaction (AR), which is triggered by an AR-inducing substance (ARIS) on a sheet-like structure. Details of the unique process of the interaction between egg jelly and sperm in these species is still unclear. The current study showed the fine structure of egg jelly in the newt Cynops pyrrhogaster, a urodele species, revealing that its outer surface was covered by a sheet-like structure of approximately 0.29 μm in thickness. Granules of approximately 2 μm in diameter with small particles of approximately 54 nm were attached to its surface and distributed inhomogeneously just beneath the sheet-like structure. Emission spectrometry revealed that the Ca2+ concentration was maintained at a high level compared with that of the blood plasma and the vas deferens fluid, suggesting that egg jelly is a reliable source of Ca2+ for the sperm–egg interaction. Blockers of the T-type voltage-dependent Ca2+ channel (VDCC), but not the L-type VDCC, inhibited both AR and initiation of sperm motility. Conversely, Ni+, which affects the α1 H subunit of T-type VDCC, only inhibited the initiation of sperm motility. These data suggest that, in response to ARIS and SMIS, sequential gating of distinct Ca2+ channels occurs in the AR, followed by the initiation of sperm motility on the surface of the egg jelly in C. pyrrhogaster at fertilization.

Cloning of cDNA for newt WT1 and the differential expression during spermatogenesis of the Japanese newt, Cynops pyrrhogaster

1998 ◽  
Vol 40 (5) ◽  
pp. 599-608 ◽  
Author(s):  
Yuki Nakayama ◽  
Takashi Yamamoto ◽  
Yoshiko Matsuda ◽  
Shin-Ichi Abe
Keyword(s):  
Differential Expression ◽  
Cynops Pyrrhogaster

The Effects of the sphragis on male and female genitalia in Acraea (Nymphalidae) butterflies

2020 ◽  
Vol 22 ◽  
Author(s):  
Shannon L Summers ◽  
Akito Y Kawahara ◽  
Ana P. S. Carvalho
Keyword(s):  
Interspecific Variation ◽  
Sperm Storage ◽  
Male Mating ◽  
Museum Collections ◽  
Storage Organ ◽  
Mating Plug ◽  
Males And Females ◽  
Comparison And Analysis ◽  
Imagej Software ◽  
Sexually Antagonistic Coevolution

Male mating plugs have been used in many species to prevent female re-mating and sperm competition. One of the most extreme examples of a mating plug is the sphragis, which is a large, complex and externalized plug found only in butterflies. This structure is found in many species in the genus Acraea (Nymphalidae) and provides an opportunity for investigation of the effects of the sphragis on the morphology of the genitalia, which is poorly understood. This study aims to understand morphological interspecific variation in the genitalia of Acraea butterflies. Using specimens from museum collections, abdomen dissections were conducted on 19 species of Acraea: 9 sphragis bearing and 10 non-sphragis bearing species. Genitalia imaging was performed for easier comparison and analysis and measurements of genitalia structures was done using ImageJ software. Some distinguishing morphological features in the females were found. The most obvious difference is the larger and more externalized copulatory opening in sphragis bearing species, with varying degrees of external projections. Females of the sphragis bearing species also tend to have a shorter ductus (the structure that connects the copulatory opening with the sperm storage organ) than those without the sphragis. These differences may be due to a sexually antagonistic coevolution between the males and females, where the females evolve larger and more difficult to plug copulatory openings and the males attempt to prevent re-mating with the sphragis.

Localization of calcium and zinc in the sperm storage tubules of chicken, quail and turkey using X-ray microanalysis

Reproduction ◽  
2000 ◽  
pp. 331-336 ◽  
Author(s):  
L Holm ◽  
H Ekwall ◽  
GJ Wishart ◽  
Y Ridderstrale
Keyword(s):  
Calcium Concentration ◽  
Sperm Storage ◽  
X Ray ◽  
Elemental Concentrations ◽  
Low Concentrations ◽  
Intracellular Content ◽  
Wet Weight ◽  
The Mean ◽  
Sperm Storage Tubules ◽  
Scanning Electron

Sperm storage tubules from the utero-vaginal junction of chickens, quails and turkeys were analysed for calcium and zinc using X-ray microanalysis of ultra-rapidly frozen tissue in a scanning electron microscope. This technique enabled the tubular fluid surrounding the stored spermatozoa and the intracellular content of the cells of the sperm storage tubules to be analysed separately and, by using standards with known concentrations, their elemental concentrations were estimated. The mean (+/- SEM) concentration of calcium in the tubular fluid from chickens, quails and turkeys was 17 +/- 3, 19 +/- 3 and 17 +/- 4 mmol kg(-1) wet weight, respectively. The intracellular calcium concentration of the cells of the tubules did not differ significantly from these values and was also similar in the mucosal epithelial cells of the utero-vaginal junction. Zinc was localized in the cells of turkey sperm storage tubules and tubular fluid, but at low concentrations. No zinc could be detected in corresponding structures from chickens and quails. The concentration of calcium in the tubular fluid is within the range known to inhibit the motility of spermatozoa, supporting this function for calcium during storage. Zinc is known to depress turkey sperm metabolism and it may also be involved in inducing quiescence of spermatozoa during storage in this species.

Molecular basis of adaptive evolution of sperm motility involved in in vivo fertilization of terrestrial animals

Impact ◽  
2020 ◽  
Vol 2020 (6) ◽  
pp. 73-75
Author(s):  
Akihiko Watanabe
Keyword(s):  
Sexual Reproduction ◽  
Sperm Motility ◽  
Asexual Reproduction ◽  
Acrosome Reaction ◽  
Sperm Morphology ◽  
Adaptive Potential ◽  
Selective Pressures ◽  
The Face ◽  
Genetic Profiles

One of the unifying traits of life on this planet is reproduction, or life's ability to make copies of itself. The mode of reproduction has evolved over time, having almost certainly begun with simple asexual reproduction when the ancestral single celled organism divided into two. Since these beginnings' life has tried out numerous strategies, and perhaps one of the most important and successful has been sexual reproduction. This form of reproduction relies on the union of gametes, otherwise known as sperm and egg. Evolutionarily, sexual reproduction allows for greater adaptive potential because the genes of two unique individuals have a chance to recombine and mix in order to produce a new individual. Unlike asexual reproduction which produces genetically-identical clones of the parent individual, sex produces offspring with novel genes and combinations of genes. Therefore, in the face of new selective pressures there is a higher chance that one of these novel genetic profiles will produce an adaptation that is advantageous in the new circumstances. Dr Akihiko Watanabe is a reproductive biologist based in the Department of Biology, Faculty of Science Yamagata University in Japan, he is currently working on three research projects; a comparative study on the signalling pathways for inducing sperm motility and acrosome reaction in amphibians, the mechanism behind the adaptive modification of sperm morphology and motility, and the origin of sperm motility initiating substance (SMIS).

TRPM8 in mouse sperm detects temperature changes and may influence the acrosome reaction

2011 ◽  
Vol 226 (6) ◽  
pp. 1620-1631 ◽  
Author(s):  
Pablo Martínez-López ◽  
Claudia L. Treviño ◽  
José Luis de la Vega-Beltrán ◽  
Gerardo De Blas ◽  
Esteban Monroy ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Temperature Changes ◽  
Mouse Sperm
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