Apoptosis and cell-cycle regulatory proteins in colorectal carcinoma: relationship to tumour stage and patient survival

2001 ◽  
Vol 194 (4) ◽  
pp. 436-443 ◽  
Author(s):  
Mohamed A. Elkablawy ◽  
Perry Maxwell ◽  
Kate Williamson ◽  
Neil Anderson ◽  
Peter W. Hamilton
Keyword(s):  
Cell Cycle ◽  
Colorectal Carcinoma ◽  
Patient Survival ◽  
Regulatory Proteins ◽  
Tumour Stage ◽  
Cell Cycle Regulatory Proteins

scholarly journals Structure-Based Virtual Screening and Biological Evaluation of Peptide Inhibitors for Polo-Box Domain

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 107 ◽  
Author(s):  
Fang Yan ◽  
Guangmei Liu ◽  
Tingting Chen ◽  
Xiaochen Fu ◽  
Miao-Miao Niu
Keyword(s):  
Cell Cycle ◽  
Virtual Screening ◽  
Fold Increase ◽  
Pharmacophore Modeling ◽  
Biological Evaluation ◽  
Peptide Inhibitors ◽  
Regulatory Proteins ◽  
Competitive Binding Assay ◽  
M Phase ◽  
Cell Cycle Regulatory Proteins

The polo-box domain of polo-like kinase 1 (PLK1-PBD) is proved to have crucial roles in cell proliferation. Designing PLK1-PBD inhibitors is challenging due to their poor cellular penetration. In this study, we applied a virtual screening workflow based on a combination of structure-based pharmacophore modeling with molecular docking screening techniques, so as to discover potent PLK1-PBD peptide inhibitors. The resulting 9 virtual screening peptides showed affinities for PLK1-PBD in a competitive binding assay. In particular, peptide 5 exhibited an approximately 100-fold increase in inhibitory activity (IC50 = 70 nM), as compared with the control poloboxtide. Moreover, cell cycle experiments indicated that peptide 5 effectively inhibited the expression of p-Cdc25C and cell cycle regulatory proteins by affecting the function of PLK1-PBD, thereby inducing mitotic arrest at the G2/M phase. Overall, peptide 5 can serve as a potent lead for further investigation as PLK1-PBD inhibitors.

scholarly journals Association of the Cell Cycle Regulatory Proteins p45SKP2and CksHs1

2001 ◽  
Vol 276 (27) ◽  
pp. 25030-25036 ◽  
Author(s):  
Lı́dia Mongay ◽  
Susana Plaza ◽  
Elena Vigorito ◽  
Carles Serra-Pagès ◽  
Jordi Vives
Keyword(s):  
Cell Cycle ◽  
Regulatory Proteins ◽  
Cell Cycle Regulatory Proteins

Effects ofHelicobacter pylori infection on cell cycle progression and the expression of cell cycle regulatory proteins

2004 ◽  
Vol 200 (3) ◽  
pp. 334-342 ◽  
Author(s):  
Antonio De Luca ◽  
Maria De Falco ◽  
Salvatore Iaquinto ◽  
Gaetano Iaquinto
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Progression ◽  
Regulatory Proteins ◽  
Cycle Progression ◽  
Cell Cycle Regulatory Proteins

Expression and modulation of map kinases and cell cycle regulatory proteins during pancreas regeneration

1998 ◽  
Vol 114 ◽  
pp. A450
Author(s):  
J. Cristobal Aliaga ◽  
E. Calvo ◽  
J. Morisset ◽  
N. Rivard
Keyword(s):  
Cell Cycle ◽  
Map Kinases ◽  
Regulatory Proteins ◽  
Pancreas Regeneration ◽  
Cell Cycle Regulatory Proteins

Cell cycle regulatory proteins in renal disease: role in hypertrophy, proliferation, and apoptosis

2000 ◽  
Vol 278 (4) ◽  
pp. F515-F529 ◽  
Author(s):  
Stuart J. Shankland ◽  
Gunter Wolf
Keyword(s):  
Cell Cycle ◽  
Renal Function ◽  
Renal Disease ◽  
Cell Injury ◽  
Critical Role ◽  
Regulatory Proteins ◽  
Specific Cell ◽  
Cell Cycle Proteins ◽  
Proliferation And Apoptosis ◽  
Cell Cycle Regulatory Proteins

The response to glomerular and tubulointerstitial cell injury in most forms of renal disease includes changes in cell number (proliferation and apoptosis) and cell size (hyerptrophy). These events typically precede and may be reponsible for the accumulation of extracellular matrix proteins that leads to a decrease in renal function. There is increasing evidence showing that positive (cyclins and cyclin-dependent kinases) and negative (cyclin-dependent kinase inhibitors) cell cycle regulatory proteins have a critical role in regulating these fundamental cellular responses to immune and nonimmune forms of injury. Data now show that altering specific cell cycle proteins affects renal cell proliferation and improves renal function. Equally exciting is the expanding body of literature showing novel biological roles for cell cycle proteins in the regulation of cell hypertrophy and apoptosis. With increasing understanding of the role for cell cyle regulatory proteins in renal disease comes the hope for potential therapeutic inverventions.

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