Application of diffusion-edited and solvent suppression 1 H-NMR to the direct analysis of markers in valerian-hop liquid herbal products

2016 ◽  
Vol 27 (2) ◽  
pp. 100-106 ◽  
Author(s):  
Jose M. Prieto ◽  
Maria Mellinas-Gomez ◽  
Mire Zloh
Keyword(s):  
Direct Analysis ◽  
Herbal Products ◽  
H Nmr ◽  
Solvent Suppression

scholarly journals Degradation of Morpholine by an EnvironmentalMycobacterium Strain Involves a Cytochrome P-450

1998 ◽  
Vol 64 (1) ◽  
pp. 159-165 ◽  
Author(s):  
P. Poupin ◽  
N. Truffaut ◽  
B. Combourieu ◽  
P. Besse ◽  
M. Sancelme ◽  
...  
Keyword(s):  
Specific Inhibitor ◽  
Direct Analysis ◽  
Difference Spectrum ◽  
Sole Source ◽  
Spectrophotometric Analysis ◽  
Treatment Unit ◽  
H Nmr ◽  
Cell Extracts ◽  
Cytochrome P 450

A Mycobacterium strain (RP1) was isolated from a contaminated activated sludge collected in a wastewater treatment unit of a chemical plant. It was capable of utilizing morpholine and other heterocyclic compounds, such as pyrrolidine and piperidine, as the sole source of carbon, nitrogen, and energy. The use of in situ1H nuclear magnetic resonance (1H NMR) spectroscopy allowed the determination of two intermediates in the biodegradative pathway, 2-(2-aminoethoxy)acetate and glycolate. The inhibitory effects of metyrapone on the degradative abilities of strain RP1 indicated the involvement of a cytochrome P-450 in the biodegradation of morpholine. This observation was confirmed by spectrophotometric analysis and1H NMR. Reduced cell extracts from morpholine-grown cultures, but not succinate-grown cultures, gave rise to a carbon monoxide difference spectrum with a peak near 450 nm, which indicated the presence of a soluble cytochrome P-450. 1H NMR allowed the direct analysis of the incubation medium containing metyrapone, a specific inhibitor of cytochrome P-450. The inhibition of morpholine degradation was dependent on the morpholine/metyrapone ratio. The heme-containing monooxygenase was also detected in pyrrolidine- and piperidine-grown cultures. The abilities of different compounds to support strain growth or the induction of a soluble cytochrome P-450 were assayed. The results suggest that this enzyme catalyzes the cleavage of the C—N bond of the morpholine ring.

Das Verhalten der Körpermasse bei einem 81-jährigen Läufer an einem 100-km-Lauf

Praxis ◽  
2009 ◽  
Vol 98 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Knechtle ◽  
Wirth ◽  
Knechtle ◽  
Kohler
Keyword(s):  
H Nmr

Ein 81-jähriger Läufer mit Status nach aortokoronarem Bypass bei bekannter koronarer Herzkrankheit hat einen 100-km-Lauf in 19 h und 45 min erfolgreich beendet. Vor und unmittelbar nach dem Lauf wurden Fett- und Muskelmasse nicht-invasiv sowohl mit der bioelektrischen Impedanzanalyse als auch mit der klassischen Hautfaltenmethode bestimmt. Zusätzlich wurden Blut- und Urinproben zur Beurteilung des Flüssigkeitshaushaltes genommen und eine ¹H-NMR-Spektroskopie des Urins zum Nachweis eines erhöhten Kohlenhydrat-, Fett- oder Eiweissstoffwechsels durchgeführt. Das Körpergewicht nahm um 1.9 kg ab. Während die errechnete Muskelmasse um 0.1 kg zunahm, nahm die errechnete Fettmasse um 0.2 kg (anthropometrische Methode) resp. 3.1 kg (BIA) ab. Das errechnete Körperwasser nahm um 1.2 l zu. Während Hämatokrit, Harnstoff und spezifisches Gewicht des Urins zunahmen, sank das Natrium im Blut ab. Das Plasmavolumen nahm um 19% ab. Die ¹H-NMR-Spektroskopie des Urins zeigte nach der Belastung einen Anstieg der Ketonkörper. Um einen Abbau der Muskelmasse objektivieren zu können, muss der Ausgangswert des Körperwassers abgewartet werden, um keine Fehlinterpretation aufgrund der Methoden zu machen. Unklar bleibt, wieso es zu einer Einlagerung von Wasser kam. In weiteren Untersuchungen könnten zusätzliche Methoden wie DEXA, Muskelbiopsien und Bestimmung von weiteren Blut- und Urinparametern Auskunft geben, ob effektiv und wie viel Muskelmasse abgebaut wird und ob Abbauprodukte der Muskulatur die Nierenfunktion einschränken und somit zu einer Wasserretention führen.

Simple and Direct Analysis of Phytosterols in Red Palm Oil by Reverse Phase HPLC and Charged Aerosol Detection

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
IN Acworth ◽  
B Bailey ◽  
M Plante ◽  
P Gamache
Keyword(s):  
Palm Oil ◽  
Direct Analysis ◽  
Reverse Phase Hplc ◽  
Reverse Phase ◽  
Red Palm Oil ◽  
Charged Aerosol Detection ◽  
Charged Aerosol

Effects of herbal products in vitro and in vivo

Planta Medica ◽  
2013 ◽  
Vol 79 (13) ◽  
Author(s):  
MJ Groot ◽  
MG Pikkemaat ◽  
WD Driessen van Lankveld
Keyword(s):  
Herbal Products

Herba Reudeuep With Modification of Heating In Virgin Cocanut Oil

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Wahyuzan Wahyuzan ◽  
Lukmanul Hakim ◽  
Rahmat Afrizal ◽  
Asmeri Lamona ◽  
Khairuni Khairuni ◽  
...  
Keyword(s):  
Modern Medicine ◽  
Heating Time ◽  
Agricultural Product ◽  
Average Yield ◽  
Herbal Products ◽  
The Public ◽  
Randomized Design ◽  
Herbal Plants ◽  
Medicinal Properties ◽  
Completely Randomized Design

Traditional medicine in Indonesia has been known by the public before formal health services using modern medicine. Indonesia has many type of herbal plants which have medicinal properties including dadap (Erythrina orientalis). This study aims to utilized dadap herbal liniment by modifying heating in Vigin Cocanut Oil. The study conducted in the laboratory of Agricultural Product Processing using a Factorial Completely Randomized Design (CRD). The first factor is the addition of 250 ml (M1), 375 ml (m2), and 400 ml (M3) Virgin Cocanut Oil treatment. The second factor is the heating tim of 5 minutes (L1), 10 minutes (L2), and 15 minutes (L3). Observation variable consisted of yield, Specific gravity, and organoleptic test of aroma and colored. The result showed an average yield of 87.70-91.88%. The highest yield was found in the treatment of adding 400 ml Virgin Cocanut Oil and 15 minutes of heating time. The highest type of weight was found in the treatment of addition of 325 ml Virgin Cocanut Oil and 5 minutes heating time. Based on the organoleptic scent test, the everage penalist gives a like answer. Organoleptic colors of reudeuep herbal products is according to SNI number 06-23-2006.

Standardization of Some Commercial Anti-diabetic Herbal Products containing Syzygiumcumini

2017 ◽  
Vol 5 (1) ◽  
pp. 21-30
Author(s):  
JASMEEN KAUR ◽  
◽  
MUNISH SINGLA ◽  
BALRAJ SAINI ◽  
GULSHAN BANSAL
Keyword(s):  
Herbal Products

Mikroalgák mint természetes hatóanyagforrások

2018 ◽  
Vol 159 (18) ◽  
pp. 703-708
Author(s):  
Gábor Vasas
Keyword(s):  
Mass Production ◽  
Higher Plants ◽  
Herbal Medicines ◽  
Point Of View ◽  
Historical Background ◽  
Herbal Products ◽  
Chlorella Sp ◽  
Economic Significance ◽  
Preclinical And Clinical Studies ◽  
Microalgal Species

Abstract: More than 90% of herbal products and herbal medicines have been derived from higher plants recently, but due to independent circumstances, several photosynthetic microalgal species are in focus in this point of view. In the last 50 years, many carbohydrate-, peptide-, terpenoid-, alkaloid- and phenol-type components were described from algae because of the developing structural determination and analytical methods, algae mass production and also artificial algae technologies. At the same time, based partly on traditional causes and partly on the clinical and preclinical data of today, some dried products of algae are directly used as food supplements. Hereinafter, the historical background, economic significance and metabolic background of the mostly used microalgal species will be reviewed. The diverse metabolite production of these organisms will be demonstrated by some molecules with special bioactivity. Several preclinical and clinical studies will be described relating to the microalgal species Spirulina sp., Chlorella sp., Haematococcus sp. and Dunaliella sp. Orv Hetil. 2018; 159(18): 703–708.

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