scholarly journals Identification of genes involved in male sterility in wheat ( Triticum aestivum  L.) which could be used in a genic hybrid breeding system

Plant Direct ◽  
2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Matthew J. Milner ◽  
Melanie Craze ◽  
Sarah Bowden ◽  
Ruth Bates ◽  
Emma J. Wallington ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Male Sterility ◽  
Breeding System ◽  
Triticum Aestivum L ◽  
Hybrid Breeding

scholarly journals The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

2019 ◽  
Vol 20 (24) ◽  
pp. 6252 ◽  
Author(s):  
Junchang Li ◽  
Jing Zhang ◽  
Huijuan Li ◽  
Hao Niu ◽  
Qiaoqiao Xu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Male Sterility ◽  
Genetic Research ◽  
Triticum Aestivum L ◽  
Anther Development ◽  
Sucrose Metabolism ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Microspore Stage ◽  
Regulation Model

Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.

scholarly journals A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)

2011 ◽  
Vol 12 (11) ◽  
pp. 943-950 ◽  
Author(s):  
Xiao-dong Chen ◽  
Dong-fa Sun ◽  
De-fu Rong ◽  
Jun-hua Peng ◽  
Cheng-dao Li
Keyword(s):  
Triticum Aestivum ◽  
Low Temperature ◽  
Male Sterility ◽  
Recessive Gene ◽  
Triticum Aestivum L

Phenotype characterisation and analysis of expression patterns of genes related mainly to carbohydrate metabolism and sporopollenin in male-sterile anthers induced by high temperature in wheat (Triticum aestivum)

2018 ◽  
Vol 69 (5) ◽  
pp. 469 ◽  
Author(s):  
Hongzhan Liu ◽  
Junsheng Wang ◽  
Chaoqiong Li ◽  
Lin Qiao ◽  
Xueqin Wang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
High Temperature ◽  
Male Sterility ◽  
Carbohydrate Metabolism ◽  
Higher Plants ◽  
Expression Patterns ◽  
Triticum Aestivum L ◽  
Male Sterile ◽  
Tetrad Stage ◽  
Expression Levels

Male reproductive development in higher plants is highly sensitive to various stressors, including high temperature (HT). In this study, physiological male-sterile plants of wheat (Triticum aestivum L.) were established using HT induction. The physiological changes and expression levels of genes mainly related to carbohydrate metabolism and sporopollenin in male-sterile processes were studied by using biological techniques, including iodine–potassium iodide staining, paraffin sectioning, scanning electron microscopy (SEM) and fluorescent quantitative analysis. Results of paraffin sectioning and SEM revealed that parts of HT male-sterile anthers, including the epidermis and tapetum, were remarkably different from those of normal anthers. The expression levels of TaSUT1, TaSUT2, IVR1 and IVR5 were significantly lower than of normal anthers at the early microspore and trinucleate stages. The RAFTIN1 and TaMS26 genes may contribute to biosynthesis and proper ‘fixation’ of sporopollenin in the development of pollen wall; however, their expression levels were significantly higher at the early tetrad stage and early microspore stage in HT sterile anthers. The recently cloned MS1 gene was expressed at the early tetrad and early microspore stages but not at the trinucleate stage. Moreover, this gene showed extremely significant, high expression in HT sterile anthers compared with normal anthers. These results demonstrate that HT induction of wheat male sterility is probably related to the expression of genes related to carbohydrate metabolism and sporopollenin metabolism. This provides a theoretical basis and technological approach for further studies on the mechanisms of HT induction of male sterility.

Relationship between metabolism of reactive oxygen species and chemically induced male sterility in wheat (Triticum aestivum L.)

2013 ◽  
Vol 93 (4) ◽  
pp. 675-681 ◽  
Author(s):  
Qing Song Ba ◽  
Gai Sheng Zhang ◽  
Jun Sheng Wang ◽  
Hui Xue Che ◽  
Hong Zhan Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Triticum Aestivum ◽  
Male Sterility ◽  
Reactive Oxygen ◽  
Triticum Aestivum L ◽  
Oxygen Species ◽  
Ros Scavenging ◽  
Male Sterile ◽  
Chemically Induced

Ba, Q. S., Zhang, G. S., Wang, J. S., Che, H. X., Liu, H. Z., Niu, N., Ma, S. C. and Wang, J. W. 2013. Relationship between metabolism of reactive oxygen species and chemically induced male sterility in wheat (Triticum aestivum L.). Can. J. Plant Sci. 93: 675–681. Chemically induced male sterility (CIMS) systems in wheat are among the male sterility types used for hybrid wheat (Triticum aestivum L.) production in China. Some studies suggested that male sterile line Xi'nong 1376-CIMS induced by chemical hybridizing agents (CHA) may suffer from oxidative stress as its cyanide-resistant respiration is lower than that of Xi'nong1376. To elucidate the metabolic mechanism of reactive oxygen species (ROS) in the CIMS anthers, the metabolism changes in the production and scavenging of ROS and gene expression related to ROS-scavenging enzymes were investigated in the anther of Xi'nong 1376-CIMS and Xi'nong1376.Anthers of Xi'nong 1376-CIMS had higher contents of [Formula: see text] and H2O2 than those of 1376, which corresponds to expression level of the NADPH oxidase (NOX) gene, and has higher contents of malondialdehyde compared with 1376. Simultaneously, there were lower activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascrodate peroxidase (APX) in scavenging ROS in the anthers of the Xi'nong 1376-CIMS line than in Xi'nong1376. Meanwhile, the expressions of SOD, POD, CAT and APX genes in 1376 were always higher at different levels than those in the Xi'nong 1376-CIMS line except for POD in stage 1. Therefore, it is possible that the sterility in Xi'nong 1376-CIMS is related to the abortion of microspores induced by chronic oxidative stress caused by an abnormal increase in ROS.

scholarly journals Chemical hybridizing agent SQ-1-induced male sterility in Triticum aestivum L.: a comparative analysis of the anther proteome

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Hongzhan Liu ◽  
Gaisheng Zhang ◽  
Junsheng Wang ◽  
Jingjing Li ◽  
Yulong Song ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Comparative Analysis ◽  
Male Sterility ◽  
Triticum Aestivum L ◽  
Chemical Hybridizing Agent

TRANSFER OF CORNERSTONE MALE-STERILITY MUTANT TO TETRAPLOID WHEAT AND HEXAPLOID AND OCTOPLOID TRITICALES

1981 ◽  
Vol 23 (3) ◽  
pp. 493-496 ◽  
Author(s):  
M. A. Hossain ◽  
C. J. Driscoll
Keyword(s):  
Triticum Aestivum ◽  
Male Sterility ◽  
Secale Cereale ◽  
Tetraploid Wheat ◽  
Colchicine Treatment ◽  
Triticum Aestivum L ◽  
Male Sterile ◽  
Ploidy Levels ◽  
Secale Cereale L ◽  
Γ Ray

A γ-ray induced male-sterility mutant on chromosome 4A of Triticum aestivum L. (Cornerstone mutant ms1c) was transferred to T. durum Desf. by backcrossing. Selfed heterozygotes of T. durum produced fewer male-sterile plants than those of T. aestivum. Male-sterile plants of T. durum and T. aestivum were crossed with diploid rye (Secale cereale L.) and fertile hexaploid and octoploid triticales were obtained following colchicine treatment of the F1's. Thus, rye is able to restore fertility at both of these ploidy levels.

Altered sucrose metabolism and gene regulation in wheat (Triticum aestivum) K-type cytoplasmic male sterility

2019 ◽  
Vol 70 (3) ◽  
pp. 204 ◽  
Author(s):  
Qingsong Ba ◽  
Lanlan Zhang ◽  
Guiping Li ◽  
Gaisheng Zhang ◽  
Hongzhan Liu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Triticum Aestivum L ◽  
Sucrose Metabolism ◽  
Vacuolar Invertase ◽  
Neutral Invertase ◽  
Cms Line ◽  
Fertile Line ◽  
Polymerase Chain

K-Type cytoplasmic male sterility (K-CMS) plays an important role in breeding hybrid wheat. This study was designed to investigate the association of sucrose metabolism with K-CMS in wheat (Triticum aestivum L.) anthers at the binucleate stage. Levels of sucrose in the anthers of the K-CMS line remained higher than in the fertile line, but glucose and fructose contents in the anthers of the K-CMS line were dramatically lower than in the fertile line. Compared with the fertile line, the activities of cell-wall-bound invertase (CWIN), neutral invertase and vacuolar invertase (VIN) were significantly reduced. Quantitative real-time polymerase chain reaction analyses showed that the expression levels of one CWIN gene (IVR1), one VIN gene (IVR5) and a sucrose transporter gene (TaSUT1) were significantly downregulated in K-CMS anthers. Furthermore, western blot confirmed that the protein expression level of IVR1 was higher in sterile anthers than in male fertile anthers. Thus, it appears that the accumulation of sucrose in K-CMS anthers might involve a decrease in activity and a reduction in content of invertase. In conclusion, the results suggest that an inability to metabolise incoming sucrose to hexoses may be involved in the K-CMS pollen-developmental lesion.

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