A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)

Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.

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Identification of genes involved in male sterility in wheat ( Triticum aestivum L.) which could be used in a genic hybrid breeding system

Plant Direct ◽

10.1002/pld3.201 ◽

2020 ◽

Vol 4 (3) ◽

Cited By ~ 1

Author(s):

Matthew J. Milner ◽

Melanie Craze ◽

Sarah Bowden ◽

Ruth Bates ◽

Emma J. Wallington ◽

...

Keyword(s):

Triticum Aestivum ◽

Male Sterility ◽

Breeding System ◽

Triticum Aestivum L ◽

Hybrid Breeding

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THE INFLUENCE OF MINERAL NUTRITION ON THE EXPRESSION OF TRAITS ASSOCIATED WITH WINTERHARDINESS OF TWO WINTER WHEAT (Triticum aestivum L.) CULTIVARS

Canadian Journal of Plant Science ◽

10.4141/cjps90-052 ◽

1990 ◽

Vol 70 (2) ◽

pp. 443-454 ◽

Cited By ~ 2

Author(s):

P. RICHARD HETHERINGTON ◽

BRYAN D. McKERSIE ◽

LISA C. KEELER

Keyword(s):

Triticum Aestivum ◽

Low Temperature ◽

Moisture Content ◽

Winter Wheat ◽

Mineral Nutrition ◽

Triticum Aestivum L ◽

Relative Ranking ◽

Acclimation Period ◽

Ice Encasement ◽

Nutrient Solutions

Two winter wheat (Triticum aestivum L.) cultivars, Fredrick and Norstar, which differ in their winterhardiness potential, were compared with regard to the effects of nitrogen (N), phosphorus (P) and potassium (K) application, during acclimation, on the expression of four traits associated with winterhardiness — freezing, ice-encasement, and low temperature flooding tolerances and crown moisture content. Modified Hoagland’s nutrient solutions containing five levels of each nutrient were applied to the seedlings during a 5-wk acclimation period at 2 °C, and subsequently the crowns were tested for their ability to survive varying intensities of the stress treatments. Increasing the level of applied N from 0, caused a reduction in the level of all stress tolerances. Increased P did not significantly alter the expression of freezing tolerance, but tended to increase tolerance of the anaerobic stresses, icing and low temperature flooding, to an optimum. Increased K had minimal effects on stress tolerance at the levels tested. Increased levels of each nutrient increased crown moisture content. The cultivar Norstar was consistently more tolerant of freezing and icing stress than Fredrick and this relative ranking was not influenced by mineral nutrition. However, the relative ranking for low temperature flooding tolerance varied depending on the nutrients provided to the seedlings. The results suggest that environmental and growth regulatory factors which influence the uptake of mineral nutrients would be expected to influence crown moisture content, and the expression of stress tolerance.Key words: Freezing, ice-encasement, flooding

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Reorganization of the microtubule and actin cytoskeleton in root cells of Triticum aestivum L. during low temperature and abscisic acid treatments

Cell Biology International ◽

10.1016/s1065-6995(02)00336-0 ◽

2003 ◽

Vol 27 (3) ◽

pp. 211-212 ◽

Cited By ~ 8

Author(s):

L Khokhlova

Keyword(s):

Triticum Aestivum ◽

Abscisic Acid ◽

Low Temperature ◽

Actin Cytoskeleton ◽

Triticum Aestivum L ◽

Root Cells ◽

Acid Treatments

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A gene for resistance to a necrosis-inducing isolate of Pyrenophora tritici-repentis located on 5BL of Triticum aestivum cv. Chinese Spring

Genome ◽

10.1139/g96-075 ◽

1996 ◽

Vol 39 (3) ◽

pp. 598-604 ◽

Cited By ~ 32

Author(s):

W. S. Stock ◽

A. L. Brûlé-Babel ◽

G. A. Penner

Keyword(s):

Triticum Aestivum ◽

Resistance Gene ◽

Chinese Spring ◽

Recessive Gene ◽

Triticum Aestivum L ◽

Tan Spot ◽

Monosomic Analysis ◽

Chromosome Location ◽

Pyrenophora Tritici Repentis ◽

Chromosome Arm

Several sources of high-level resistance to tan spot caused by Pyrenophora tritici-repentis have been identified in hexaploid wheat (Triticum aestivum L.). This study was conducted to determine the number and chromosome location of a gene(s) in the cultivar Chinese Spring (CS) that confers resistance to a tan necrosis inducing isolate (nec+chl−) of P. tritici-repentis, 86-124, and insensitivity to Ptr necrosis toxin. Reciprocal crosses were made between CS (resistant–insensitive) and 'Kenya Farmer' (KF) (susceptible–sensitive). Analysis of the CS/KF F1and F2 populations and F2-derived F3 families identified a single nuclear recessive gene governing resistance to isolate 86-124 and Ptr necrosis toxin. Evaluation of the CS(KF) substitution series, F2 monosomic analysis, and screening of a series of 19 CS compensating nullitetrasomic and two ditelosomic lines (2AS and 5BL) indicated that the resistance gene was located on chromosome arm 5BL. No linkage exists between Lr18 and the tan necrosis resistance gene on chromosome arm 5BL. It is proposed that the gene for resistance to the tan necrosis inducing isolate 86-124 (nec+chl−) of P. tritici-repentis and Ptr necrosis toxin be named tsn1. Key words : wheat, Triticum aestivum L., tan spot resistance, Pyrenophora tritici-repentis (Died.) Drechs., chromosome location, Ptr necrosis toxin.

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Phenotype characterisation and analysis of expression patterns of genes related mainly to carbohydrate metabolism and sporopollenin in male-sterile anthers induced by high temperature in wheat (Triticum aestivum)

Crop and Pasture Science ◽

10.1071/cp18034 ◽

2018 ◽

Vol 69 (5) ◽

pp. 469 ◽

Cited By ~ 4

Author(s):

Hongzhan Liu ◽

Junsheng Wang ◽

Chaoqiong Li ◽

Lin Qiao ◽

Xueqin Wang ◽

...

Keyword(s):

Triticum Aestivum ◽

High Temperature ◽

Male Sterility ◽

Carbohydrate Metabolism ◽

Higher Plants ◽

Expression Patterns ◽

Triticum Aestivum L ◽

Male Sterile ◽

Tetrad Stage ◽

Expression Levels

Male reproductive development in higher plants is highly sensitive to various stressors, including high temperature (HT). In this study, physiological male-sterile plants of wheat (Triticum aestivum L.) were established using HT induction. The physiological changes and expression levels of genes mainly related to carbohydrate metabolism and sporopollenin in male-sterile processes were studied by using biological techniques, including iodine–potassium iodide staining, paraffin sectioning, scanning electron microscopy (SEM) and fluorescent quantitative analysis. Results of paraffin sectioning and SEM revealed that parts of HT male-sterile anthers, including the epidermis and tapetum, were remarkably different from those of normal anthers. The expression levels of TaSUT1, TaSUT2, IVR1 and IVR5 were significantly lower than of normal anthers at the early microspore and trinucleate stages. The RAFTIN1 and TaMS26 genes may contribute to biosynthesis and proper ‘fixation’ of sporopollenin in the development of pollen wall; however, their expression levels were significantly higher at the early tetrad stage and early microspore stage in HT sterile anthers. The recently cloned MS1 gene was expressed at the early tetrad and early microspore stages but not at the trinucleate stage. Moreover, this gene showed extremely significant, high expression in HT sterile anthers compared with normal anthers. These results demonstrate that HT induction of wheat male sterility is probably related to the expression of genes related to carbohydrate metabolism and sporopollenin metabolism. This provides a theoretical basis and technological approach for further studies on the mechanisms of HT induction of male sterility.

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Genome-wide identification of CCT genes in wheat (Triticum aestivum L.) and their expression analysis during vernalization

PLoS ONE ◽

10.1371/journal.pone.0262147 ◽

2022 ◽

Vol 17 (1) ◽

pp. e0262147

Author(s):

HongWei Zhang ◽

Bo Jiao ◽

FuShuang Dong ◽

XinXia Liang ◽

Shuo Zhou ◽

...

Keyword(s):

Triticum Aestivum ◽

Low Temperature ◽

Winter Wheat ◽

Expression Analysis ◽

Gene Clusters ◽

Triticum Aestivum L ◽

Whole Wheat ◽

Genome Wide ◽

Leaf Tissues ◽

Extended Exposure

Numerous CCT genes are known to regulate various biological processes, such as circadian rhythm regulation, flowering, light signaling, plant development, and stress resistance. The CCT gene family has been characterized in many plants but remains unknown in the major cereal wheat (Triticum aestivum L.). Extended exposure to low temperature (vernalization) is necessary for winter wheat to flower successfully. VERNALIZATION2 (VRN2), a specific CCT-containing gene, has been proved to be strongly associated with vernalization in winter wheat. Mutation of all VRN2 copies in three subgenomes results in the eliminated demands of low temperature in flowering. However, no other CCT genes have been reported to be associated with vernalization to date. The present study screened CCT genes in the whole wheat genome, and preliminarily identified the vernalization related CCT genes through expression analysis. 127 CCT genes were identified in three subgenomes of common wheat through a hidden Markov model-based method. Based on multiple alignment, these genes were grouped into 40 gene clusters, including the duplicated gene clusters TaCMF6 and TaCMF8, each tandemly arranged near the telomere. The phylogenetic analysis classified these genes into eight groups. The transcriptome analysis using leaf tissues collected before, during, and after vernalization revealed 49 upregulated and 31 downregulated CCT genes during vernalization, further validated by quantitative real-time PCR. Among the differentially expressed and well-investigated CCT gene clusters analyzed in this study, TaCMF11, TaCO18, TaPRR95, TaCMF6, and TaCO16 were induced during vernalization but decreased immediately after vernalization, while TaCO1, TaCO15, TaCO2, TaCMF8, and TaPPD1 were stably suppressed during and after vernalization. These data imply that some vernalization related CCT genes other than VRN2 may exist in wheat. This study improves our understanding of CCT genes and provides a foundation for further research on CCT genes related to vernalization in wheat.

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