scholarly journals Genetic manipulation of trehalose‐6‐phosphate synthase results in changes in the soluble sugar profile in transgenic sugarcane stems

Plant Direct ◽  
2021 ◽  
Vol 5 (11) ◽  
Author(s):  
Casey Gabriel ◽  
Jean‐Jacque Fernhout ◽  
Franziska Fichtner ◽  
Regina Feil ◽  
John E. Lunn ◽  
...  
Keyword(s):  
Genetic Manipulation ◽  
Soluble Sugar ◽  
Transgenic Sugarcane ◽  
Sugar Profile ◽  
Phosphate Synthase

scholarly journals Effects of CEPA and 1-MCP on flower bud differentiation of apple cv. ‘Nagafu No.2’ grafted on different rootstocks

2018 ◽  
Author(s):  
Wenfang Li ◽  
Baihong Chen ◽  
Juan Mao ◽  
Xinwen Li ◽  
Jing Su ◽  
...  
Keyword(s):  
Sucrose Synthase ◽  
Soluble Sugar ◽  
Sucrose Phosphate Synthase ◽  
Flower Formation ◽  
Flower Bud ◽  
Juvenile Period ◽  
Significant Difference ◽  
Flowering Rate ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

AbstractThe apple (Malus domestica Borkh.) has a relatively long juvenile period which prevent the fruit breeding. The understanding of the flowering system is important to improve breeding efficiency in the apple. In this context, 2-year-old “Fuji” apple cv. “Nagafu No.2” trees that were grafted on dwarf self-rooted rootstock M.26, vigorous rootstock M. sieversii and interstock M.26/M. sieversii, respectively. Spraying with clean water (as controls), 800 mg·L−1 2-Chloroethylphosphonic acid (CEPA) and 2 μL·L−1 1-methylcyclopropene (1-MCP). The results showed that CEPA significantly repressed the vegetative growth attributed to the increase of the ABA and ZT synthesis, and the decrease of IAA synthesis in leaves and buds. However, there was no significant difference or significant inverse effect between 1-MCP and control. Furthermore, CEPA promoted flower formation, increased the flowering rate and advanced the blossom period for 2 days compared with the control, which accompanied by the accumulation of soluble sugar, glucose and sucrose, and the increase of α-amylase (α-AMY) and sucrose phosphate synthase (SPS) activities, and the decrease of the starch contents and sucrose synthase (SS) activities in leaves and buds. However, the blossom period was delayed for 2 days after spraying with 1-MCP. Finally, the expression of TFL1 was significantly repressed while the AP1 was significantly promoted in buds from M.26 and M.26/M. sieversii after spraying with CEPA, while the effect was not significant from M. sieversii. However, the expression levels of TFL1 and AP1 were not significantly different from the control after the application of 1-MCP. In spite of this, CEPA was more susceptible to easy-flowering M26, followed by M26/M. sieversii, and still less susceptible to difficult-flowering rootstock M. sieversii.Abbreviations1-MCP1-methylcyclopropeneα-amylase(α-AMY)ABAabscisic acidCEPA2-Chloroethylphosphonic acidCTKcytokininsETHethyleneGAgibberellinSPSsucrose phosphate synthaseSSsucrose synthaseZTzeatin.

scholarly journals TRANSFORMASI GEN SUCROSE PHOSPHATE SYNTHASE (SoSPS1) MENGGUNAKAN Agrobacterium tumefaciens UNTUK MENINGKATKAN SINTESIS SUKROSA PADA TANAMAN TEBU (Saccharum officinarum L.)

2007 ◽  
Vol 12 (2) ◽  
pp. 137-143
Author(s):  
Miswar Miswar ◽  
Bambang Sugiharto ◽  
Joedoro Soedarsono ◽  
Sukarti Moeljapawiro
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Starch Content ◽  
Sucrose Phosphate Synthase ◽  
Saccharum Officinarum ◽  
Invertase Activity ◽  
Pcr Analysis ◽  
Sucrose Synthesis ◽  
Transgenic Sugarcane ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose phosphate synthase (SPS EC 2.3.1.14) plays an important role in partition of assimilated carbon in most plants. SPS catalyses the penultimate reaction in the pathway of sucrose synthesis, in which sucrose-6-phosphate (Suc6P) is synthesized from UDPglucose (UDPG) and fructose-6-P (Fru6P). To increase the capacity of sugarcane in sucrose synthesis, spindle leaves of sugarcane cv R579 were transformed with cDNA SoSPS1 from sugarcane under the control of constitutive promoter (35S CaMV) that constructed in pBI 121 (pKYS) using Agrobacterium tumefaciens. Based on PCR analysis, we have detected the existence of SPS transgene in some lines of transformed sugarcane, called line 4, 5, 6, and 7. The SPS transgene in transformed sugarcane could be expressed into translation level and increased the amount of leaves SPS protein, so the activity of leaves SPS was higher than wild type sugarcane as control. The transformed sugarcane line 4, 5, 6, and 7 showed 1.4–2.9 fold increases in SPS activity and 1,76–2,2 fold increases in leaves sucrose content. Increasing in SPS activity in transgenic sugarcane was coupled by the increase in invertase activity and ratio between sucrose and starch content.

scholarly journals Effects of Nighttime Heating on Cell Size, Acid Invertase Activity, Sucrose Phosphate Synthase Activity, and Sugar Content of Melon Fruit

2010 ◽  
Vol 135 (6) ◽  
pp. 501-505 ◽  
Author(s):  
Jun Matsumoto ◽  
Hideyuki Goto ◽  
Yasutaka Kano ◽  
Akira Kikuchi ◽  
Hideaki Ueda ◽  
...  
Keyword(s):  
Cell Size ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Sucrose Phosphate Synthase ◽  
Invertase Activity ◽  
Acid Invertase ◽  
Sugar Accumulation ◽  
Control Fruit ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

To determine the relationship among cell size, acid invertase (AI) activity, sucrose phosphate synthase (SPS) activity, and sucrose accumulation in melon (Cucumis melo L.) during early development [from 6 to 16 days after anthesis (DAA)], fruit were heated at night to a minimum of 20 °C. Cells of heated fruit were larger than those of control fruit at 16 DAA but smaller at 50 DAA. AI activity was lower and SPS activity was higher in heated than in control fruit up to 26 DAA. Sucrose, glucose, and fructose contents at 26 and 50 DAA were higher in heated than in control fruit. Heating caused cells to reach mature size earlier than those of control fruit, and maturity was accompanied by earlier decline in AI activity and an earlier increase in SPS activity that promoted soluble sugar accumulation.

Determination of soluble sugar profile in rice

2017 ◽  
Vol 1058 ◽  
pp. 19-23 ◽  
Author(s):  
Xianqiao Hu ◽  
Changyun Fang ◽  
Lin Lu ◽  
Zhanqiang Hu ◽  
Yafang Shao ◽  
...  
Keyword(s):  
Soluble Sugar ◽  
Sugar Profile

scholarly journals Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 200 ◽  
Author(s):  
Risky Mulana Anur ◽  
Nurul Mufithah ◽  
Widhi Dyah Sawitri ◽  
Hitoshi Sakakibara ◽  
Bambang Sugiharto
Keyword(s):  
Sugar Content ◽  
Sucrose Phosphate Synthase ◽  
Sucrose Content ◽  
Transgenic Sugarcane ◽  
Soluble Acid Invertase ◽  
Sugarcane Yield ◽  
Transgenic Lines ◽  
Key Enzyme ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sucrose phosphate synthase (SPS) is a key enzyme in sucrose synthesis, which controls sucrose content in plants. This study was designed to examine the efficacy of the overexpression of SoSPS1 gene on sucrose accumulation and carbon partitioning in transgenic sugarcane. The overexpression of SoSPS1 gene increased SPS activity and sucrose content in transgenic sugarcane leaves. More importantly, the overexpression enhanced soluble acid invertase (SAI) activity concomitant with the increase of glucose and fructose levels in the leaves, whereas sucrose synthase activity exhibited almost no change. In the stalk, a similar correlation was observed, but a higher correlation was noted between SPS activity and sugar content. These results suggest that SPS overexpression has both direct and indirect effects on sugar concentration and SAI activity in sugarcane. In addition, SPS overexpression resulted in a significant increase in plant height and stalk number in some transgenic lines compared to those in non-transgenic control. Taken together, these results strongly suggest that enhancing SPS activity is a useful strategy for improving sugarcane yield.

scholarly journals Development of Allergenicity and Toxicity Assessment Methods for Evaluating Transgenic Sugarcane Overexpressing Sucrose–Phosphate Synthase

Agronomy ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
Intan Ria Neliana ◽  
Widhi Dyah Sawitri ◽  
Netty Ermawati ◽  
Tri Handoyo ◽  
Bambang Sugiharto
Keyword(s):  
Sucrose Phosphate Synthase ◽  
Specific Ige ◽  
Toxicity Assessment ◽  
Sugarcane Juice ◽  
Human Consumption ◽  
Oral Toxicity ◽  
Transgenic Sugarcane ◽  
Allergenic Proteins ◽  
Sucrose Phosphate ◽  
Phosphate Synthase

Sugarcane is considered as an industrial crop that produces sugar. The number of transgenic sugarcane on the market is currently increasing. Therefore, investigation of the potential allergens and toxics in transgenic sugarcane is necessary, since there is less information regarding food safety for human consumption. Bioinformatics and experimental analysis were used for the validation of the allergenic potential of transgenic sugarcane overexpressing sucrose–phosphate synthase (SPS). Bioinformatics analysis showed that SPS has no homology with any known allergenic proteins. However, eight-residues identical contiguous sequence was detected, and further specific assessment is required to confirm the potential of allergenicity. The results of protein stability evaluation showed that SPS gradually decreased at 28 °C and rapidly inactivated at 60 °C and 90 °C by heat treatment. In addition, total protein was degraded by simulated gastric fluids (SGF), and simulated intestine fluid (SIF) assays for one-minute incubation. The level of specific IgE in the transgenic sugarcane and controls also showed no potential risk of allergy. An acute oral toxicity assay was performed by oral gavage of transgenic sugarcane juice in mice. The LD50 for transgenic sugarcane juice was >25 gr/kg body weight. We propose a development method for allergenicity and toxicity assessment in transgenic sugarcane.

Kinetic studies on soluble sugar profile in rice during storage: Derivation using the Laplace transform

2022 ◽  
pp. 102915
Author(s):  
Yaqiu Wang ◽  
Yuanrong Zheng ◽  
Ran Zhou ◽  
Ming Ma
Keyword(s):  
Laplace Transform ◽  
Soluble Sugar ◽  
Kinetic Studies ◽  
The Laplace Transform ◽  
Sugar Profile

Metabolic engineering of CHO cells to prepare glycoproteins

2018 ◽  
Vol 2 (3) ◽  
pp. 433-442 ◽  
Author(s):  
Qiong Wang ◽  
Michael J. Betenbaugh
Keyword(s):  
Metabolic Engineering ◽  
Cho Cells ◽  
Genetic Manipulation ◽  
Chinese Hamster ◽  
Post Translational Modification ◽  
Effector Functions ◽  
Recombinant Glycoproteins ◽  
Production Platform ◽  
Chemical Inhibitors ◽  
Amino Acid Mutations

As a complex and common post-translational modification, N-linked glycosylation affects a recombinant glycoprotein's biological activity and efficacy. For example, the α1,6-fucosylation significantly affects antibody-dependent cellular cytotoxicity and α2,6-sialylation is critical for antibody anti-inflammatory activity. Terminal sialylation is important for a glycoprotein's circulatory half-life. Chinese hamster ovary (CHO) cells are currently the predominant recombinant protein production platform, and, in this review, the characteristics of CHO glycosylation are summarized. Moreover, recent and current metabolic engineering strategies for tailoring glycoprotein fucosylation and sialylation in CHO cells, intensely investigated in the past decades, are described. One approach for reducing α1,6-fucosylation is through inhibiting fucosyltransferase (FUT8) expression by knockdown and knockout methods. Another approach to modulate fucosylation is through inhibition of multiple genes in the fucosylation biosynthesis pathway or through chemical inhibitors. To modulate antibody sialylation of the fragment crystallizable region, expressions of sialyltransferase and galactotransferase individually or together with amino acid mutations can affect antibody glycoforms and further influence antibody effector functions. The inhibition of sialidase expression and chemical supplementations are also effective and complementary approaches to improve the sialylation levels on recombinant glycoproteins. The engineering of CHO cells or protein sequence to control glycoforms to produce more homogenous glycans is an emerging topic. For modulating the glycosylation metabolic pathways, the interplay of multiple glyco-gene knockouts and knockins and the combination of multiple approaches, including genetic manipulation, protein engineering and chemical supplementation, are detailed in order to achieve specific glycan profiles on recombinant glycoproteins for superior biological function and effectiveness.

Metabonomic study of transgenic and non-transgenic sugarcane leaves based on NMR profile

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
T Lira ◽  
JH Yariwake ◽  
YH Choi ◽  
HK Kim ◽  
R Verpoorte
Keyword(s):  
Transgenic Sugarcane

Drug-Induced Agranulocytosis: A Mini Review

2016 ◽  
Vol 2 (1) ◽  
pp. 57-59
Author(s):  
Pavithra D ◽  
Praveen D ◽  
Vijey Aanandhi M
Keyword(s):  
Bone Marrow ◽  
Complete Blood Count ◽  
Genetic Manipulation ◽  
White Blood Cells ◽  
Deep Infection ◽  
Morbidity Rate ◽  
Drug Induced ◽  
Serious Adverse Event ◽  
Mortality And Morbidity ◽  
The Individual

Agranulocytosis is also known to be granulopenia, causing neutropenia in circulating blood streams .The destruction of white blood cells takes place which leads to increase in the infection rate in an individual where immune system of the individual is suppressed. The symptoms includes fever, sore throat, mouth ulcers. These are commonly seen as adverse effects of a particular drug and are prescribed for the common diagnostic test for regular monitoring of complete blood count in an admitted patient. Drug-induced agranulocytosis remains a serious adverse event due to occurrence of severe sepsis with deep infection leading to pneumonia, septicaemia, and septic shock in two/third of the patient. Antibiotics seem to be the major causative weapon for this disorder. Certain drugs mainly anti-thyroid drugs, ticlopidine hydrochloride, spironolactone, clozapine, antileptic drugs (clozapine), non-steroidal anti-inflammatory agents, dipyrone are the potential causes. Bone marrow insufficiency followed by destruction or limited proliferative bone marrow destruction takes place. Chemotherapy is rarely seen as a causative agent for this disorder. Genetic manipulation may also include as one of the reason. Agranulocytosis can be recovered within two weeks but the mortality and morbidity rate during the acute phase seems to be high, appropriate adjuvant treatment with broad-spectrum antibiotics are prerequisites for the management of complicated neutropenia. Drugs that are treated for this are expected to change as a resistant drug to the patient. The pathogenesis of agranulocytosis is not yet known. A comprehensive literature search has been carried out in PubMed, Google Scholar and articles pertaining to drug-induced agranulocytosis were selected for review.

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