scholarly journals Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research

2011 ◽  
Vol 5 (3-4) ◽  
pp. 109-120 ◽  
Author(s):  
Joao A. Paulo ◽  
Linda S. Lee ◽  
Bechien Wu ◽  
Peter A. Banks ◽  
Hanno Steen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chronic Pancreatitis ◽  
Pancreatic Fluid

Transient Stasis of Pancreatic Fluid Flow Together with Mild Injury of the Pancreatic Duct Cause Chronic Pancreatitis

2011 ◽  
Vol 56 (8) ◽  
pp. 2326-2332 ◽  
Author(s):  
Taizo Yamaguchi ◽  
Yasuyuki Kihara ◽  
Mitsuyoshi Yamamoto ◽  
Makoto Otsuki
Keyword(s):  
Fluid Flow ◽  
Chronic Pancreatitis ◽  
Pancreatic Duct ◽  
Pancreatic Fluid

scholarly journals Elevated Polyamines in Saliva of Pancreatic Cancer

2018 ◽  
Author(s):  
Yasutsugu Asai ◽  
Takao Itoi ◽  
Masahiro Sugimoto ◽  
Atsushi Sofuni ◽  
Takayoshi Tsuchiya ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pancreatic Cancer ◽  
Capillary Electrophoresis ◽  
Chronic Pancreatitis ◽  
Screening Test ◽  
High Accuracy ◽  
The Other ◽  
Non Invasive ◽  
Accurate Detection ◽  
Significant Difference

Detection of pancreatic cancer (PC) at a resectable stage is still difficult because of the lack of accurate detection tests. The development of accurate biomarkers in low or non-invasive biofluids is essential to enable frequent tests, which would help increase the opportunity of PC detection in early stages. Polyamines have been reported as possible biomarkers in urine and saliva samples in various cancers. Here, we analyzed salivary metabolites, including polyamines, using capillary electrophoresis-mass spectrometry. Salivary samples were collected from patients with PC (n=39), chronic pancreatitis (CP, n=14) and controls (C, n=26). Polyamines, such as spermine, N1-acetylspermidine, and N1-acetylspermine, showed a significant difference between PC and C, and the combination of four metabolites including N1-acetylspermidine showed high accuracy in discriminating PC from the other two groups. These data showed the potential of saliva as a screening test for PC.

scholarly journals Identification and validation of a multivariable prediction model based on blood plasma and serum metabolomics for the distinction of chronic pancreatitis subjects from non-pancreas disease control subjects

Gut ◽  
2021 ◽  
pp. gutjnl-2020-320723
Author(s):  
M Gordian Adam ◽  
Georg Beyer ◽  
Nicole Christiansen ◽  
Beate Kamlage ◽  
Christian Pilarsky ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chronic Pancreatitis ◽  
Ethylenediaminetetraacetic Acid ◽  
External Validation ◽  
Gas Chromatography Mass Spectrometry ◽  
Imaging Features ◽  
Total N ◽  
Increased Risk ◽  
Edta Plasma ◽  
Bayes Algorithm

ObjectiveChronic pancreatitis (CP) is a fibroinflammatory syndrome leading to organ dysfunction, chronic pain, an increased risk for pancreatic cancer and considerable morbidity. Due to a lack of specific biomarkers, diagnosis is based on symptoms and specific but insensitive imaging features, preventing an early diagnosis and appropriate management.DesignWe conducted a type 3 study for multivariable prediction for individual prognosis according to the TRIPOD guidelines. A signature to distinguish CP from controls (n=160) was identified using gas chromatography-mass spectrometry and liquid chromatography‐tandem mass spectrometry on ethylenediaminetetraacetic acid (EDTA)-plasma and validated in independent cohorts.ResultsA Naive Bayes algorithm identified eight metabolites of six ontology classes. After algorithm training and computation of optimal cut-offs, classification according to the metabolic signature detected CP with an area under the curve (AUC) of 0.85 ((95% CI 0.79 to 0.91). External validation in two independent cohorts (total n=502) resulted in similar accuracy for detection of CP compared with non-pancreatic controls in EDTA-plasma (AUC 0.85 (95% CI 0.81 to 0.89)) and serum (AUC 0.87 (95% CI 0.81 to 0.95)).ConclusionsThis is the first study that identifies and independently validates a metabolomic signature in plasma and serum for the diagnosis of CP in large, prospective cohorts. The results could provide the basis for the development of the first routine laboratory test for CP.

Lipidomic Profiling of Serum and Pancreatic Fluid in Chronic Pancreatitis

Pancreas ◽  
2012 ◽  
Vol 41 (4) ◽  
pp. 518-522 ◽  
Author(s):  
Tyler Stevens ◽  
Michael P. Berk ◽  
Rocio Lopez ◽  
Yoon-Mi Chung ◽  
Renliang Zhang ◽  
...  
Keyword(s):  
Chronic Pancreatitis ◽  
Pancreatic Fluid

scholarly journals Animal Models of Chronic Pancreatitis

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Makoto Otsuki ◽  
Mitsuyoshi Yamamoto ◽  
Taizo Yamaguchi
Keyword(s):  
Fluid Flow ◽  
Oleic Acid ◽  
Chronic Pancreatitis ◽  
Animal Models ◽  
Pancreatic Duct ◽  
Sodium Taurocholate ◽  
Pancreatic Injury ◽  
The Other ◽  
Fatty Tissue ◽  
Pancreatic Fluid

Animal models for CP in rats can be classified into 2 groups: one is noninvasive or nonsurgical models and the other is invasive or surgical models. Pancreatic injury induced by repetitive injections of supramaximal stimulatory dose of caerulein (Cn) or by intraductal infusion of sodium taurocholate (NaTc) recovered within 14 days, whereas that caused by repetitive injection of arginine or by intraductal infusion of oleic acid was persistent. However, the destroyed acinar tissues were replaced by fatty tissues without fibrosis. Transient stasis of pancreatic fluid flow by 0.01% agarose and minimum injury of the pancreatic duct by 0.1% NaTc solution induced progressive pancreatic injury although one alone is insufficient to cause persistent pancreatic injury. However, the damaged tissue was replaced by fatty tissue without fibrosis. Continuous pancreatic ductal hypertension (PDH) caused diffuse interlobular and intralobular fibrosis closely resembling human CP.

scholarly journals Dysphagia Relieved by Endoscopic Transpapillary Pancreatic Duct Stent Placement!

2019 ◽  
Vol 10 (01) ◽  
pp. 053-055
Author(s):  
Surinder Singh Rana ◽  
Ravi Sharma ◽  
Sobur Uddin Ahmed ◽  
Sonali Guleria ◽  
Rajesh Gupta
Keyword(s):  
Chronic Pancreatitis ◽  
Pancreatic Duct ◽  
Stent Placement ◽  
Rare Case ◽  
Pancreatic Fluid Collections ◽  
Pancreatic Fluid ◽  
Pancreatic Duct Stent ◽  
Pancreatic Duct Disruption ◽  
Fluid Collections ◽  
Duct Disruption

ABSTRACTPancreatic fluid collections are usually peripancreatic in location but can be found at various atypical locations such as the mediastinum. Mediastinal pseudocysts are very rare and are very unusual cause of dysphagia. Here, we report a rare case of mediastinal pseudocyst occuring because of pancreatic duct disruption due to chronic pancreatitis and presenting as dysphagia and successfully treated with endoscopic transpapillary stent placement.

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