Indel-based targeting of essential proteins in human pathogens that have close host orthologue(s): Discovery of selective inhibitors for Leishmania donovani elongation factor-1α

2007 ◽  
Vol 67 (1) ◽  
pp. 53-64 ◽  
Author(s):  
Devki Nandan ◽  
Martin Lopez ◽  
Fuqiang Ban ◽  
Meilan Huang ◽  
Yvonne Li ◽  
...  
2020 ◽  
Vol 58 (11) ◽  
Author(s):  
Nicky Didwania ◽  
Sarfaraz Ahmad Ejazi ◽  
Rudra Chhajer ◽  
Abdus Sabur ◽  
Saumyabrata Mazumder ◽  
...  

ABSTRACT Visceral leishmaniasis (VL) is a threat in many developing countries. Much effort has been put to eliminating this disease, for which serodiagnosis remains the mainstay for VL control programs. New and improved antigens as diagnostic candidates are required, though, as the available antigens fail to demonstrate equal optimum performance in all areas of endemicity. Moreover, these diagnoses are dependent on invasive serum sampling. In the current study, we cloned and expressed Leishmania donovani cysteine protease C (CPC) and evaluated its diagnostic and test-of-cure possibilities by detecting the antibody levels in human serum and urine through ELISA and immunoblot assays. Two immunodominant antigens, recombinant glycoprotein 63 (GP63) and elongation factor 1α (EF1α), identified earlier by our group, were also assessed by employing human serum and urine samples. Of these three antigens in ELISAs, CPC demonstrated the highest sensitivities of 98.15% and 96% positive testing in serum and urine of VL patients, respectively. Moreover, CPC yielded 100% specificity with serum and urine of nonendemic healthy controls compared to GP63 and EF1α. Urine samples were found to be more specific than serum for distinguishing endemic healthy controls and other diseases by means of all three antigens. In all cases, CPC gave the most promising results. Unlike serum, urine tests demonstrated a significant decrease in antibody levels for CPC, GP63, and EF1α after 6 months of treatment. The diagnostic and test-of-cure performances of CPC in the immunoblot assay were found to be better than those of GP63 and EF1α. In conclusion, CPC, followed by GP63 and EF1α, may be utilized as candidates for diagnosis of VL and to assess treatment response.


Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
E Xingi ◽  
D Smirlis ◽  
S Bisti ◽  
V Myrianthopoulos ◽  
P Magiatis ◽  
...  

FEBS Letters ◽  
1999 ◽  
Vol 453 (1-2) ◽  
pp. 29-34 ◽  
Author(s):  
Masumi Nakazawa ◽  
David Moreira ◽  
Jacqueline Laurent ◽  
Hervé Le Guyader ◽  
Yasuo Fukami ◽  
...  

2014 ◽  
Vol 42 (22) ◽  
pp. 14042-14052 ◽  
Author(s):  
Kosuke Ito ◽  
Takayoshi Honda ◽  
Takahiro Suzuki ◽  
Tomohiro Miyoshi ◽  
Ryo Murakami ◽  
...  

Biochemistry ◽  
2002 ◽  
Vol 41 (2) ◽  
pp. 628-633 ◽  
Author(s):  
Mariorosario Masullo ◽  
Piergiuseppe Cantiello ◽  
Barbara de Paola ◽  
Francesca Catanzano ◽  
Paolo Arcari ◽  
...  

Genome ◽  
2019 ◽  
Vol 62 (3) ◽  
pp. 160-169 ◽  
Author(s):  
Wieland Meyer ◽  
Laszlo Irinyi ◽  
Minh Thuy Vi Hoang ◽  
Vincent Robert ◽  
Dea Garcia-Hermoso ◽  
...  

With new or emerging fungal infections, human and animal fungal pathogens are a growing threat worldwide. Current diagnostic tools are slow, non-specific at the species and subspecies levels, and require specific morphological expertise to accurately identify pathogens from pure cultures. DNA barcodes are easily amplified, universal, short species-specific DNA sequences, which enable rapid identification by comparison with a well-curated reference sequence collection. The primary fungal DNA barcode, ITS region, was introduced in 2012 and is now routinely used in diagnostic laboratories. However, the ITS region only accurately identifies around 75% of all medically relevant fungal species, which has prompted the development of a secondary barcode to increase the resolution power and suitability of DNA barcoding for fungal disease diagnostics. The translational elongation factor 1α (TEF1α) was selected in 2015 as a secondary fungal DNA barcode, but it has not been implemented into practice, due to the absence of a reference database. Here, we have established a quality-controlled reference database for the secondary barcode that together with the ISHAM-ITS database, forms the ISHAM barcode database, available online at http://its.mycologylab.org/ . We encourage the mycology community for active contributions.


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