Inhibitory effect against polymerase and ribonuclease activities of HIV-reverse transcriptase of the aqueous leaf extract ofTerminalia triflora

2002 ◽  
Vol 16 (8) ◽  
pp. 778-780 ◽  
Author(s):  
V. S. Martino ◽  
P. López ◽  
J. J. Martinez Irujo ◽  
M. Sanromán ◽  
M. T. Cuevas ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Hiv Reverse Transcriptase ◽  
Aqueous Leaf Extract

A Homodimeric Sporamin-Type Trypsin Inhibitor with Antiproliferative, HIV Reverse Transcriptase-Inhibitory and Antifungal Activities from Wampee (Clausena lansium) Seeds

2003 ◽  
Vol 384 (2) ◽  
pp. 289-293 ◽  
Author(s):  
T.B. Ng ◽  
S.K. Lam ◽  
W.P. Fong
Keyword(s):  
Reverse Transcriptase ◽  
Trypsin Inhibitor ◽  
Simple Procedure ◽  
Inhibitory Effect ◽  
Exchange Chromatography ◽  
Proteinase K ◽  
Human Leukemia ◽  
Hiv Reverse Transcriptase ◽  
Rabbit Reticulocyte Lysate System ◽  
Reticulocyte Lysate

Abstract A homodimeric trypsin inhibitor with a molecular mass of 54 kDa was isolated from the seeds of Clausena lansium (Lour) Skeels with a very simple procedure comprising extraction with an aqueous buffer and ion exchange chromatography on CM-cellulose. It inhibited trypsin with an IC50 of 2.2 nM but was without any inhibitory effect on chymotrypsin and proteinase K. The uptake of MTT by human leukemia HL60 and hepatoma Hep G2 cells was inhibited with an IC50 of 100 uM. Translation in the cellfree rabbit reticulocyte lysate system was inhibited with an IC50 of 3.6 uM. The activity of HIV-1 reverse transcriptase was reduced in the presence of the trypsin inhibitor. The trypsin inhibitor exerted antifungal activity toward Physalospora piricola but not Mycosphaerella arachidicola, Botrytis cinerea, Fusarium oxysporum or Coprinus comatus.

Anti-Aids Agents, 2: Inhibitory Effect of Tannins on HIV Reverse Transcriptase and HIV Replication in H9 Lymphocyte Cells

1990 ◽  
Vol 53 (3) ◽  
pp. 587-595 ◽  
Author(s):  
Gen-ichiro Nonaka ◽  
Itsuo Nishioka ◽  
Makoto Nishizawa ◽  
Takashi Yamagishi ◽  
Yoshiki Kashiwada ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Inhibitory Effect ◽  
Hiv Reverse Transcriptase ◽  
Hiv Replication

scholarly journals Studies on Allelopathic Effect of Aqueous Leaf Extract of Putranjiva Roxburghii Wall. on Seed Germination and Early Growth of Chickpea (Cicer Arietinum L.)

2019 ◽  
Author(s):  
Satyajit Oraon ◽  
Subrata Mondal
Keyword(s):  
Seed Germination ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Early Growth ◽  
Growth Stages ◽  
Allelopathic Effect ◽  
Leaf Extracts ◽  
Cicer Arietinum L ◽  
Aqueous Leaf Extract ◽  
Putranjiva Roxburghii

The present study reveals the allelopathic effect of aqueous leaf extracts of Putranjiva roxburghii Wall. on seed germination and early growth stages of an economically important plant chickpea (Cicer arietinum L.). Aqueous leaf extracts at 0, 5, 10, 15, 25, 35, 50, 75 and 100% concentrations were applied to determine their effect on seed germination and early growth stages under laboratory conditions. Laboratory-based experiments with three replicates were used to arrange treatments accordingly. After 5 days of incubation the rate of seed germination, radicle length and hypocotyls length etc. of chickpea seeds were observed after treatment with aqueous leaf extracts. Highest inhibitory effect was noticed in 100% of aqueous leaf extract. Results indicated that the inhibitory effect was proportional to the concentration of the extracts. The water soluble leaf extracts contain allelochemicals, which inhibit the seed germination and reduced the early growth of chickpea.

RNA aptamers and HIV reverse transcriptase : molecular basis of their interactions and the evaluation of aptamer resistance

2019 ◽  
Author(s):  
◽  
Phuong Dinh Minh Nguyen
Keyword(s):  
Reverse Transcriptase ◽  
Reverse Transcription ◽  
Immune Deficiency Syndrome ◽  
Inhibitory Effect ◽  
Proteolytic Processing ◽  
Rna Aptamers ◽  
Rna Aptamer ◽  
Hiv Reverse Transcriptase ◽  
Functional Features ◽  
Hiv Rt

[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Human Immunodeficiency virus (HIV) is a pandemic Lentivirus that causes acquired immune deficiency syndrome (AIDS) in infected individuals. One of the critical steps in HIV replication is the reverse transcription of viral RNA to generate viral DNA, which will be integrated into the host genome to generate materials for the production of new viruses. The enzyme responsible for this reverse transcription process is HIV reverse transcriptase (RT). Because of its importance in HIV life cycle, RT has been a common target for HIV inhibitors. In recent years, RNA aptamers have emerged as potential inhibitors against HIV RT. Although previous studies had shown that the inhibitory effect of RNA aptamers against HIV RT comes from their ability to compete with primer/template for binding to RT, the molecular details of RT-RNA aptamer interaction are still limited. The work described herein highlights advancements in identifying the functional features of a broad-spectrum RNA aptamer and elucidating the molecular details in the interaction of this aptamer and the target RT. Furthermore, this work explores the potential use of anti HIV aptamers in studying RT maturation by proteolytic processing.

scholarly journals Inhibitory Effect of 2,3,5,6-Tetrafluoro-4-[4-(aryl)-1H-1,2,3-triazol-1-yl]benzenesulfonamide Derivatives on HIV Reverse Transcriptase Associated RNase H Activities

2016 ◽  
Vol 17 (8) ◽  
pp. 1371 ◽  
Author(s):  
Nicolino Pala ◽  
Francesca Esposito ◽  
Dominga Rogolino ◽  
Mauro Carcelli ◽  
Vanna Sanna ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Inhibitory Effect ◽  
Rnase H ◽  
Hiv Reverse Transcriptase

scholarly journals Effect of Different Concentrations of Aqueous Leaf Extracts of Some Plants on the Germination and Seedling Growth of Maize, Z. mays L. and Wheat (T. aestivum L.)

2014 ◽  
Vol 3 (3) ◽  
pp. 264-274
Author(s):  
Rizwan Ali Khan ◽  
Kaiser Iqbal ◽  
Aatif Hussain ◽  
Showkat Azeem
Keyword(s):  
Triticum Aestivum ◽  
Leaf Extract ◽  
Root Elongation ◽  
Zea Mays L ◽  
Inhibitory Effect ◽  
Allelopathic Effect ◽  
Food Crops ◽  
Leaf Extracts ◽  
Leaf Leachate ◽  
Aqueous Leaf Extract

Allelopathic effect of aqueous leaf extract of three different species (eucalyptus, guava, and litchi) were used to investigate their effect on germination, shoot and root lengths of two food crops viz. Zea mays L. (Maize) and Triticum aestivum (Wheat). Mature fresh leaves of three species were crushed and soaked for 24h; the filtrates were diluted to make different concentrations (T1 = 0.5%, T2 = 1.0%, T3 = 1.5%, T4 = 2.0%, and T5 = 0.0%), and were used to investigate their effect on the tested crops. The aqueous leaf leachate of three species was found to have inhibitory effect on germination, shoot, and root elongation on the tested crops. Current study indicates that inhibitory effect was much more pronounced at higher concentrations, and the effect increased with the increase in concentration. Among trees, Eucalyptus has much more effect on the tested crops than other species. DOI: http://dx.doi.org/10.3126/ije.v3i3.11085 International Journal of Environment Vol.3(3) 2014: 264-274

Cytoprotective and antiadhesive effects of aqueous leaf extract from Orthosiphon aristatus against uropathogenic E. coli

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381 ◽  
Author(s):  
S Sarshar ◽  
MR Asadi Karam ◽  
M Habibi ◽  
S Bouzari ◽  
X Qin ◽  
...  
Keyword(s):  
Leaf Extract ◽  
E Coli ◽  
Aqueous Leaf Extract

Insecticidal Activities of the Leaf Extract of the Invasive Alien Plant, Chromolaena odorata (L.) (Asteraceae) Against Macrotermes species (Termites)

2019 ◽  
Vol 35 (1) ◽  
pp. 145-153
Author(s):  
O. Uyi, ◽  
I.G. Amolo ◽  
A.D. Adetimehin
Keyword(s):  
Leaf Extract ◽  
Insect Pests ◽  
Chromolaena Odorata ◽  
Leaf Extracts ◽  
Alien Plant ◽  
Biological Efficacy ◽  
Invasive Alien Plant ◽  
Impregnation Technique ◽  
Aqueous Leaf Extract ◽  
Worker Caste

Several studies have demonstrated the biological efficacy of leaf, stem and root powders or extracts of Chromolaena odorata (L.) King and Robinson against insect pests but those that are focused on the biological efficacy of aqueous leaf extracts against Macrotermes species are scanty. Current management of termites with synthetic insecticides is being discouraged due to human and environmental hazards. Therefore, the insecticidal effectiveness of aqueous leaf extract C. odorata against Macrotermes species was investigated. Five concentrations (0, 2.5, 5.0, 7.5 and 10.0% (w/v)) of the aqueous extract of C. odorata plant were evaluated for repellency and toxicity on the worker caste of Macrotermes species following standard procedures. The filter paper impregnation technique was used for the bioassay. Percentage repellency was monitored for 30 minutes and mortality recorded at 12, 24 and 36 hours post exposure. The leaf extract of C. odorata significantly repelled 95% of Macrotermes species at the highest concentration of 10% (w/v) after 30 minutes post treatment exposure. Mortality of Macrotermes species was independent of treatment concentration, but dependent on duration of exposure. All treatment concentrations of aqueous leaf extract of C. odorata caused significant mortality against Macrotermes species ranging between 94% and 98% compared to the control; indicating very great potential for adoption and use in the management of Macrotermes species.

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