Insecticidal Activities of the Leaf Extract of the Invasive Alien Plant, Chromolaena odorata (L.) (Asteraceae) Against Macrotermes species (Termites)

2019 ◽  
Vol 35 (1) ◽  
pp. 145-153
Author(s):  
O. Uyi, ◽  
I.G. Amolo ◽  
A.D. Adetimehin
Keyword(s):  
Leaf Extract ◽  
Insect Pests ◽  
Chromolaena Odorata ◽  
Leaf Extracts ◽  
Alien Plant ◽  
Biological Efficacy ◽  
Invasive Alien Plant ◽  
Impregnation Technique ◽  
Aqueous Leaf Extract ◽  
Worker Caste

Several studies have demonstrated the biological efficacy of leaf, stem and root powders or extracts of Chromolaena odorata (L.) King and Robinson against insect pests but those that are focused on the biological efficacy of aqueous leaf extracts against Macrotermes species are scanty. Current management of termites with synthetic insecticides is being discouraged due to human and environmental hazards. Therefore, the insecticidal effectiveness of aqueous leaf extract C. odorata against Macrotermes species was investigated. Five concentrations (0, 2.5, 5.0, 7.5 and 10.0% (w/v)) of the aqueous extract of C. odorata plant were evaluated for repellency and toxicity on the worker caste of Macrotermes species following standard procedures. The filter paper impregnation technique was used for the bioassay. Percentage repellency was monitored for 30 minutes and mortality recorded at 12, 24 and 36 hours post exposure. The leaf extract of C. odorata significantly repelled 95% of Macrotermes species at the highest concentration of 10% (w/v) after 30 minutes post treatment exposure. Mortality of Macrotermes species was independent of treatment concentration, but dependent on duration of exposure. All treatment concentrations of aqueous leaf extract of C. odorata caused significant mortality against Macrotermes species ranging between 94% and 98% compared to the control; indicating very great potential for adoption and use in the management of Macrotermes species.

scholarly journals Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy

2017 ◽  
Vol 57 (2) ◽  
pp. 194-200 ◽  
Author(s):  
Abdul A. Buhroo ◽  
Gousul Nisa ◽  
Syed Asrafuzzaman ◽  
Ram Prasad ◽  
Razia Rasheed ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Average Particle ◽  
Particle Sizes ◽  
Mythimna Separata ◽  
Ag Nps ◽  
Leaf Extracts ◽  
Tem Analysis ◽  
Vis Spectroscopy ◽  
Aqueous Leaf Extract

AbstractThe present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) usingTrichodesma indicumaqueous leaf extract as a reducing agent. The synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. The photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. The green synthesis of spherical-shaped Ag NPs bio-fabricated fromT. indicumwith a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal efficacy of biosynthesized Ag NPs with leaf extracts ofT. indicumagainstMythimna separata. The results showed that Ag NPs (20–50 nm) ofT. indicumpossess good larvicidal activity againstM. separatawith an LC50of 500 ppm. Thus, we can advocate that Ag NPs of 20–50 nm size extracted fromT. indicummay be considered in the pest management programme ofM. separatain future.

scholarly journals Evaluation of qualitative phytochemicals and antifungal activity of aqueous leaf extract of Senna tora on downy mildew of cabbage

2018 ◽  
Vol 11 (1) ◽  
pp. 15-19
Author(s):  
S.M. Zakari ◽  
A.A. Aliko ◽  
H Haruna ◽  
A Muazu
Keyword(s):  
Downy Mildew ◽  
Leaf Extract ◽  
Flavonoid Glycosides ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Active Metabolites ◽  
Leaf Extracts ◽  
Minimum Fungicidal Concentration ◽  
Significant Difference ◽  
Aqueous Leaf Extract

This study was embarked upon to evaluate the efficacy of aqueous extracts of Senna tora leaf against Perenospora parasitica, causing downy mildew on cabbage. Phytochemical analysis of the leaf extracts revealed the presence of some secondary metabolites (Tannins, Saponins, Flavonoid, Glycosides, Alkaloids, Phenolic compounds and Carbohydrates) which have been reported to have antimicrobial effects. Assays were performed using extract concentrations of 1000, 500, 250, 125 mg/ml by agar well diffusion technique. Results obtained, revealed a significant difference in diameter of mycelial zone of inhibition between the concentrations. Highest zone of inhibition was recorded at 1000 and 500 mg/ml (20 and 15 mm) respectively. Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) were determined and recorded at 250 mg/ml. The antifungal screening revealed that the extract exhibited inhibitory activities at varying concentrations against the downy mildew pathogen. These activities observed could be attributed to the presence of active metabolites contained in the extract.Keywords: Aqueous leaf extract, Downy mildew, Perenospora parasitica and Senna tora

scholarly journals In Vivo Antidiabetic Efficacy, Mineral Element Composition, and Qualitative Phytochemistry of the Aqueous Leaf Extracts of Pentas zanzibarica (Klotzsch.) Vatke and Olea europaea subspecies africana (Mill.)

2021 ◽  
Author(s):  
Gervason Apiri Moriasi ◽  
Cromwell Mwiti Kibiti ◽  
Mathew Piero Ngugi
Keyword(s):  
Body Weight ◽  
Elemental Composition ◽  
Plant Extracts ◽  
Leaf Extract ◽  
Absorption Spectrometry ◽  
High Morbidity ◽  
Leaf Extracts ◽  
Aqueous Leaf Extract ◽  
Pharmacologically Active

Abstract Persistent hyperglycaemia is the hallmark of diabetes and is accountable for the devastating complications, which cause high morbidity and mortality. Conventional anti-diabetic agents are only palliative and characterised with limited efficacy, adverse effects, high costs, inaccessibility, prompting the need for better alternatives. Therefore, we investigated the in vivo hypoglycaemic activities, elemental composition, and qualitative phytochemistry of the aqueous leaf extracts of P. zanzibarica and O. europaea subspecies africana as potential sources of affordable, safer, accessible, and potent anti-diabetic therapies. In vivo hypoglycaemic activities of the studied plant extracts were evaluated at three dose levels of 50, 100, and 150 mg/Kg body weight (bw) in Alloxan-induced hyperglycaemic Swiss albino mice. The elemental composition of the plant extracts was analysed using the energy dispersive x-ray fluorescence spectroscopy (EDXRF System) and the atomic absorption spectrometry (AAS). Qualitative phytochemical screening was done following standard procedures. In this study, the aqueous leaf extract of P. zanzibarica, significantly (p<0.05) reduced alloxan-induced hyperglycaemia in mice from 163.26±2.24 mg/dL (at 0h) to 52.12±1.16 mg/dL (at the 4th h) at a dose of 50 mg/Kg body weight, 166.98±1.56 mg/dL (at 0h) to 48.90±1.40 mg/dL (at 4th h) at a dose of 100 mg/Kg body weight, and 168.64±2.96 mg/dL (at 0h) to 42.46±1.80 mg/dL (at 4th h) at a dose of 150 mg/Kg body weight, respectively. Similarly, the aqueous leaf extract of O. europaea subspecies africana significantly (p<0.05) reduced hyperglycaemia from 165.74±3.66 mg/dL (at 0h) to 65.26±1.46 mg/dL (at the 4th h) at a dose of 50 mg/Kg body weight, 158.14±3.49 mg/dL (at 0h) to 53.68±1.48 mg/dL (at the 4th h) at a dose of 100 mg/Kg body weight, and from 161.66±2.19 mg/dL (at 0h) to 44.48±1.35 mg/dL (at the 4th h) at a dose of 150 mg/Kg body weight, respectively Furthermore, the extracts contained chromium (Cr), zinc (Zn), magnesium (Mg), among other elements, and phytochemicals like phenols, flavonoids, and alkaloids, among others. Generally, the studied plant extracts exhibited significant hypoglycaemic efficacy in alloxan-induced hyperglycaemic mice, indicating their antidiabetic potential, and possess pharmacologically active phytochemicals and valuable minerals.

scholarly journals Phytochemical Screening and Oral Acute Toxicity Study of Aqueous Leaf Extract of Crinum giganteum (Gadalli) in Wister Rats

2016 ◽  
Vol 8 ◽  
pp. 22
Author(s):  
Elizabeth FB ◽  
Obikili E.N ◽  
Esom A. E ◽  
Anyanwu G.E
Keyword(s):  
Leaf Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Acute Toxicity Study ◽  
Phytochemical Compounds ◽  
Aqueous Leaf Extract ◽  
Bioactive Agents ◽  
Oral Acute Toxicity ◽  
Bioactive Agent

<p><strong>Background:</strong> Different parts of plants possess variable phytochemical compounds and LD<sub>50</sub>. <em>Crinum giganteum</em> (Gadalli) is one of the plants most commonly consumed in parts of Africa for its various medicinal values. Despite this popularity, the phytochemical screening and the toxicity of the leaf extracts were yet to be evaluated. This study was designed to identify    the bioactive agents and estimate the LD<sub>50</sub> for the aqueous leaf extract of gadalli. <strong>Method</strong>: Phytochemical screening was done using standard methods. Each bioactive agent and the LD<sub>50</sub> were estimated by Arithmetic methods of Karber. <strong>Results: </strong>Phytochemical analysis revealed high presence of alkaloids,  saponins, and slight presence of glycosides while the oral LD<sub>50 </sub>was found to be 200 mg/kg. <strong>Conclusion: </strong>The major active ingredients of<strong> </strong>aqueous leaf extract of gadalli are alkaloids and saponins. It is observed to be unsafe at 200mg/kg and above.</p><p><strong>Keywords: </strong>Phytochemicals, <em>Crinum Giganteum</em>, LD<sub>50</sub>, Alkaloids, Saponins, Glycosides</p><p> </p>

scholarly journals Antimicrobial Activity of Chromolaena odorata Extracts against Bacterial Human Skin Infections

2016 ◽  
Vol 10 (2) ◽  
pp. 159 ◽  
Author(s):  
Srisuda Hanphanphoom ◽  
Sukhumaporn Krajangsang
Keyword(s):  
Antimicrobial Activity ◽  
Human Skin ◽  
Enterococcus Faecalis ◽  
Inhibitory Activity ◽  
Leaf Extract ◽  
Skin Infections ◽  
Chromolaena Odorata ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Staph Aureus

<p>Leaf, stem and root of <em>Chromolaena odorata</em> were extracted by maceration extraction method using water, ethanol, methanol and hexane as solvents. The average percentage yield of leaf extracts in water (12.16 ± 0.13%), ethanol (8.42 ± 0.115%), methanol (10.45 ± 0.012%) and hexane (2.37 ± 0.215%) were significantly higher compared with stem and root extracts using the same solvents. All extracts were tested for antimicrobial activity against ten bacterial strains associated with human skin infections. Leaf extracts with ethanol, methanol and hexane solvents gave the best inhibitory activity against six gram-positive bacterial strains (<em>Bacillus cereus</em> TISTR 687, <em>Enterococcus faecalis </em>TISTR 379, <em>Staphylococcus epidermidis </em>TISTR 518, <em>Staphylococcus aureus </em>TISTR 1466, <em>Streptococcus pyogenes </em>ATCC 19615 and <em>Propionibacterium acnes</em> DMST 14916) and one gram-negative bacterial strain (<em>Proteus vulgaris </em>ATCC 13315). The hexane stem extract showed greater inhibitory activity against <em>Pseudomonas aeruginosa </em>ATCC 27853(15.3±0.5 mm), <em>B. cereus</em> TISTR 687(14.6±0.8 mm) and <em>Klebsiella pneumoniae</em> TISTR 1843 (14.0±1.0 mm), while hexane root extract showed high inhibitory activity against <em>Enterococcus faecalis </em>TISTR 379 (14.5±0.9 mm) and <em>Kleb. pneumoniae</em> TISTR 1843 (14.7±0.6 mm). The lowest minimum inhibitory concentration (MIC) of the ethanolic leaf extract was 0.81 mg/mL against <em>Staph. aureus </em>TISTR 1466, followed by methanolic and hexane leaf extracts with equal MIC of 1.62 mg/mL against both <em>Staph. aureus </em>TISTR 1466 and <em>Strep. pyogenes </em>ATCC 19615. The antimicrobial activity of the methanolic leaf extract of <em>C. odorata </em>was correlated with the amount of both total phenolic and flavonoid compounds. The results obtained suggest that the ethanolic, methanolic and hexane leaf extracts could be developed to treat bacterial skin infections. The hexane was sutiable solvent for extraction of the stem and root parts of <em>C. odorata</em>.</p>

scholarly journals BIOPESTISIDA DARI EKSTRAK DAUN PANGI (Pangium sp.) TERHADAP SERANGGA Plutella xylostella DI SULAWESI UTARA

EUGENIA ◽  
2012 ◽  
Vol 18 (3) ◽  
Author(s):  
Christina L. Salaki ◽  
Evie Paendong ◽  
Jantje Pelealu
Keyword(s):  
Plutella Xylostella ◽  
Leaf Extract ◽  
Insect Pests ◽  
Feeding Activity ◽  
Crop Protection ◽  
Botanical Insecticides ◽  
Leaf Extracts ◽  
North Sulawesi ◽  
Environmental Friendly ◽  
One Way Anova

ABSTRACT   Searching the plants in which can produce biopesticides, such as antifeedant to control the insect pests, it attracted the attention of worldwide researches. It is due to in the crop protection, the compounds of antifeedant do not kill, repel or entrap insects, but just inhibit the insect's appetite, so that the food crops or the commodity can be protected. Plutella xylostella (L) is the insect pests which is cosmopolitantly distributed in. Its attacks could damage vegetables resulting in loss of quantitative and qualitative. To overcome these problems, need to develop a means of pest control, which are effective but environmental friendly. North Sulawesi has a lot of plants, which is potentially developed as a source of botanical insecticides. Pangi Plant (Pangium sp.) is a plant species which potentially developed and effective against several types of insect pests, but testing by using crude extract can give varies results depending on the type of extract used, the test insects and environment factors. The purpose of this study was to determine the potential of leaf extracts pangi (Pangium sp.) as antifeedant against Plutella xylostella. The method used is the method of Qin et al. (2004). To determine the class of active compounds to extract the thick antifeedant have done by taking each extract to make a concentrations of 1% (w / v), 5% (w / v), and 10% (w / v). Data were analyzed by Analysis of Variance (One-way ANOVA). The results showed that, the leaf extract of condensed pangi were able to inhibit the feeding activity of the larvae of P. xylostella. Keywords : biopesticide, Pangium sp., Plutella xylostella ABSTRAK   Penelusuran tumbuh-tumbuhan yang dapat menghasilkan biopestisida, seperti anti makan (antifeedant) untuk mengendalikan hama serangga, sangat menarik perhatian para peneliti di seluruh dunia. Hal ini disebabkan karena dalam perlindungan tanaman, senyawa anti makan tidak membunuh, mengusir atau menjerat serangga hama, tetapi hanya menghambat selera makan dari serangga tersebut, sehingga tanaman pangan atau komoditi dapat terlindungi. Plutella xylostella (L) merupakan serangga hama yang penyebarannya bersifat kosmopolitan. Serangan ini dapat merusak tanaman sayuran mengakibatkan kehilangan hasil secara kuantitatif maupun kualitatif. Untuk mengatasi permasalahan tersebut, perlu dikembangkan sarana pengendalian hama yang efektif tetapi ramah lingkungan. Sulawesi Utara memiliki banyak tumbuhan yang berpotensi untuk dikembangkan sebagai sumber insektisida botani. Tanaman pangi (Pangium sp.) adalah  jenis tumbuhan yang berpotensi untuk dikembangkan dan efektif terhadap beberapa jenis serangga hama, namun pengujian dengan menggunakan ekstrak kasar dapat memberikan hasil yang beragam tergantung dari jenis ekstrak yang digunakan, faktor serangga uji dan lingkungan. Tujuan penelitian ini adalah untuk mengetahui potensi ekstrak daun pangi (Pangium sp.) sebagai anti makan terhadap P. xylostella. Metode yang digunakan adalah  metode Qin et al. (2004). Untuk mengetahui golongan senyawa aktif anti makan terhadap ekstrak kental dilakukan dengan mengambil masing-masing ekstrak untuk dibuat konsentrasi 1 % (b/v), 5 % (b/v), dan 10 % (b/v). Data dianalisa dengan analisis varians (One-way Anova). Hasil penelitian menunjukkan bahwa Ekstrak kental daun pangi mampu menghambat aktivitas makan dari larva P. xylostella.  Eugenia Volume 18  No. 3  Desember 2012 Kata Kunci : biopestisida, Pangium sp., Plutella xylostella

scholarly journals Studies on Allelopathic Effect of Aqueous Leaf Extract of Putranjiva Roxburghii Wall. on Seed Germination and Early Growth of Chickpea (Cicer Arietinum L.)

2019 ◽  
Author(s):  
Satyajit Oraon ◽  
Subrata Mondal
Keyword(s):  
Seed Germination ◽  
Leaf Extract ◽  
Inhibitory Effect ◽  
Early Growth ◽  
Growth Stages ◽  
Allelopathic Effect ◽  
Leaf Extracts ◽  
Cicer Arietinum L ◽  
Aqueous Leaf Extract ◽  
Putranjiva Roxburghii

The present study reveals the allelopathic effect of aqueous leaf extracts of Putranjiva roxburghii Wall. on seed germination and early growth stages of an economically important plant chickpea (Cicer arietinum L.). Aqueous leaf extracts at 0, 5, 10, 15, 25, 35, 50, 75 and 100% concentrations were applied to determine their effect on seed germination and early growth stages under laboratory conditions. Laboratory-based experiments with three replicates were used to arrange treatments accordingly. After 5 days of incubation the rate of seed germination, radicle length and hypocotyls length etc. of chickpea seeds were observed after treatment with aqueous leaf extracts. Highest inhibitory effect was noticed in 100% of aqueous leaf extract. Results indicated that the inhibitory effect was proportional to the concentration of the extracts. The water soluble leaf extracts contain allelochemicals, which inhibit the seed germination and reduced the early growth of chickpea.

scholarly journals Invasive Alien Plants in Sub-Saharan Africa: A Review and Synthesis of Their Insecticidal Activities

2021 ◽  
Vol 3 ◽  
Author(s):  
Osariyekemwen Uyi ◽  
Ludzula Mukwevho ◽  
Afure J. Ejomah ◽  
Michael Toews
Keyword(s):  
Plant Species ◽  
Insecticidal Activity ◽  
Insect Pests ◽  
Sub Saharan Africa ◽  
Future Research ◽  
African Countries ◽  
Alien Plant ◽  
Invasive Alien Plant ◽  
Plant Parts ◽  
Sub Saharan

Despite the cornucopia of agricultural, economic and ecological ramifications of invasive alien plant species (IAPs) in sub-Saharan Africa, studies on their potential use as bio-insecticides have not received adequate attention compared to the burgeoning plethora of literature on their use in ethnomedicine. In the current study, we review the existing, but scattered literature on the insecticidal activity of different parts of some IAPs; specifically those invasive in sub-Saharan Africa but with published literature from Africa and elsewhere. From our literature survey, we found that 69 studies from four continents (Africa, Asia, North America and South America) reported the insecticidal activity of 23 plant species from 13 families (Asteraceae = 6 species; Solanaceae = 3 species; Apocynacee, Fabaceae and Euphorbiaceae 2 species each; Araceae, Bignoniaceae, Chenopodiaceae, Meliaceae, Mimosaceae, Myrtaceae, Papaveraceae, and Verbenaceae = 1 species each) that are invasive in, and alien to Africa. The highest number of published case studies were from India (n = 19) and Nigeria (n = 15). We found that varying concentrations of extracts or powders from different plant parts caused 50–100% mortality against a myriad of insect pests of agriculture and environmental importance. Our review discussed the prospects for exploiting IAPs as pesticidal plants in African countries especially among resource-poor small-holder farmers and locals to improve agricultural productivity and livelihoods. Finally, we highlighted safety concerns and challenges of using IAPs as bio-insecticides in Africa and formulates appropriate recommendations for future research.

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