Aqueous Extracts of Wild Mushrooms Show Antimicrobial and Antiadhesion Activities against Bacteria and Fungi

The aim of the present study was to determine the degree of microbial contamination of aqueous extracts of green and roasted coffee originating in India. The samples used in this analysis were obtained by two extraction methods: hot extraction and cold extraction. If a microbial load of samples was found, the same extracts were filtered using sterile 0.22 μm pore size filters from PES (Polyester) for decontamination. The working methodology was based on the Standard SR EN ISO 21149/2017 which provides general guidelines for counting and detecting mesophilic aerobic bacteria using as a medium for isolation, cultivation and counting of microorganisms (bacteria and fungi) the non-differential environment. The experimental results obtained showed microbial contamination of all samples. The level of microbial contamination is different for the extracts taken under study, depending on the type of coffee used, green or roasted, but also on other conditions, such as wet or dry processing. Roasted coffee is much less contaminated compared to green coffee, due to a thermal (incomplete) sterilization by the roasting procedure. The working conditions used, the temperature of the distilled water used to prepare the extracts, their non-sterile handling, but also the type of coffee used, are the factors that determined the microbial contamination.

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Comparative Antimicrobial Effect of Boswellia, Lichen, Coffea peel, Asafoetida, Saussurea Costus, Rhatany, Anise and Cumin Extracts on Some Animal Pathogens

Open Access Journal of Veterinary Science & Research ◽

10.23880/oajvsr-16000193 ◽

2020 ◽

Vol 5 (1) ◽

pp. 1-9

Author(s):

Rehab Mohamed Atta El-Desoukey

Keyword(s):

Antimicrobial Effect ◽

Diffusion Method ◽

Aqueous Extracts ◽

Antimicrobial Compound ◽

Cuminum Cyminum ◽

Pimpinella Anisum ◽

Animal Pathogens ◽

Antifungal Action ◽

Bacteria And Fungi ◽

Agar Well Diffusion Method

Numerous plants have so far been utilized for the treatment and the executives of different illnesses since the start of human development. One of the regular issues in the clinical world, spreading of bacterial opposition against anti-infection agents, so one of the most significant strides in microbiological investigates is to locate another antimicrobial compound with insignificant reactions. Because of the nearness of organic dynamic mixes in plant and herbs and its utilization in conventional Objective medication and sustenance, it appears that this plants and herbs contain limit significant antimicrobial. So the aim of this study is to explore the antimicrobial action of Boswellia, Lichen, Coffea peel, Rhatany (Krameria triandra), Cuminum cyminum, Pimpinella anisum, Asafoetida, and Saussurea costus aqueous and solvent extracts on some therapeutically significant bacteria and fungi isolated from animals. Hot and cold aqueous extracts in addition to alcohol extracts of Boswellia, Lichen, Coffea peel, Rhatany (Krameria triandra), Cuminum cyminum, Pimpinella anisum , Asafoetida,and Saussurea costus were assessed for their antimicrobial effect against Streptococcus, Escherichia coli Salmonella, klebsiella and Candida albicans by agar well diffusion method. Every single examined plants and herbs indicated antibacterial impact against examined pathogenic microscopic organisms particularly Rhatany, Asafoetida, Lichen, and Pimpinella anisum. The lesser impact was for Coffea peel. Also the most antifungal action against Candida albican swas for Rhatany. For the most part the viable concentrate was the cold aqueous extract for all analyzed plants and herbs.

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Evaluation of the Acetone and Aqueous Extracts of Mature Stem Bark ofSclerocarya birreafor Antioxidant and Antimicrobial Properties

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/834156 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Nicoline F. Tanih ◽

Roland N. Ndip

Keyword(s):

Antioxidant Activities ◽

Bacterial Species ◽

Radical Scavenging ◽

Antimicrobial Properties ◽

Reducing Power ◽

Stem Bark ◽

Acetone Extract ◽

Aqueous Extracts ◽

Reducing Ability ◽

Bacteria And Fungi

We assayed the antimicrobial activity of acetone and aqueous extracts of the stem bark ofSclerocarya birreaon some selected bacteria and fungi species including;Streptococcus pyogenes, Plesiomonas shigelloides,Aeromonas hydrophila, Salmonella typhimurium, Cryptococcus neoformans, Candida glabrata, Trichosporon mucoides,andCandida kruseiusing both agar well diffusion and minimum inhibitory concentration (MIC) assays. Based on the levels of activity, the acetone extract was examined for total polyphenolic content, radical scavenging and antioxidant activities. Total phenols of the extract were determined spectrophotometrically. The antioxidant activity was determined by the DPPH, ABTS and reducing power. All the bacteria and fungi species were susceptible to the plant extracts. The acetone extract was the most active for the bacterial species with MIC (0.156–0.625 mg/mL) while the aqueous extract was the most active for the fungi species with MIC (0.3125–1.25 mg/mL). The polyphenolic compounds were found as 27.2 mg/g tannic acid equivalent, 25.2 mg/g quercetin equivalent, 9.1 mg/g quercetin equivalent for phenols, flavonoid and flavonols respectively. The acetone extract exhibited a remarkable ability to scavenge radicals, strong reducing ability and a potential source of natural antioxidants. Both the acetone and aqueous extracts ofS. birreamay provide a target for drug discovery.

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