Changes of Carbohydrate Composition During Enzymatic Hydrolysis of Starch with Mycolase Participation

The purpose of the work is to study the enzymatic hydrolysis of inulin-containing syrups of various purification degrees from chicory chips in the production of oligofructose to be used in dietary, diabetic and health-preventive nutrition products. It has been determined that ion exchange purification of the syrup is necessary for hydrolysis. Individual stages of ion-exchange purification are specified using a two-stage scheme: К1-А1-К2-А2 and an additional stage on the “Macronet” sorbent MN200 to stabilize pH and remove bitterness taste. Requirements for the quality of syrup for hydrolysis have been developed: pH value — 4.5–5.0; chromaticity — not more than 0.5 units opt. den.; ash — not more than 0.2%; protein — 0.5%; no bitterness taste. Optimal conditions for hydrolysis of inulin-containing syrup have been established using Novozim 960 endoinulinase (Denmark): temperature — 55–58 °C; pH — 4.7–5.2; DS (dry substance) — 19%; preparation dosage — 0.4 units. INU/g of syrup DS; time 20–24 h. A sample of oligofructose syrup was obtained using the preparation “Novozim 960.” The carbohydrate composition of oligofructose after the inulin hydrolysis was determined: fructooligosaccharides (FOS) — 70.12%; oligofructosides — 24.79%; disaccharides — 2.11%; fructose — 2.98%. Requirements for carbohydrate composition of oligofructose obtained by enzymatic hydrolysis of inulin-containing syrup have been developed: sum total of FOS and oligosaccharides — not less than 93%, sum total of di- and monosaccharides — not more than 7%.

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INFLUENCE OF HYDROLYTIC TREATMENT ON THE CONTENT OF REDUCING SUBSTANCES IN NEUTRAL-SULPHITE ALKALI

chemistry of plant raw material ◽

10.14258/jcprm.2021039160 ◽

2021 ◽

pp. 309-317

Author(s):

Leysan Azatovna Mingazova ◽

Yelena Vyacheslavovna Kryakunova ◽

Zosia Albertovna Kanarskaya ◽

Альберт Владимирович Kanarskiy ◽

Igor' Vadimovich Kruchina-Bogdanov ◽

...

Keyword(s):

Liquid Chromatography ◽

Enzymatic Hydrolysis ◽

Acid Hydrolysis ◽

Dry Matter ◽

Gas Liquid Chromatography ◽

Birch Wood ◽

Carbohydrate Composition ◽

Enzyme Preparations ◽

Reducing Substances ◽

Hydrolysis Of

The aim of this work is to develop a technology for the preparation of neutral-sulfite liquors formed during the production of fibrous semi-finished products - cellulose from birch wood - for subsequent use as a nutrient medium for the cultivation of microorganisms. Acid hydrolysis was carried out at a temperature of 100 °С at a ratio of a 10% sulfuric acid solution to a liquor sample of 1 : 1. Enzymatic hydrolysis of neutral sulfite liquors was carried out with the enzyme preparations Accellerase XY and Accellerase XC at 50±2 °C and 60±2 °C. The end of hydrolysis was determined by the cessation of the increase in the content of reducing substances (RS) in the hydrolyzate. The original neutral sulphite lye contained 9.4% dry matter, 21.7 g/l of reducing substances, pH 5.3±0.2. It has been shown that as a result of enzymatic hydrolysis, the content of insoluble dry residue in the hydrolyzate decreases to 8.32% and 8.41%, respectively, and during acid hydrolysis – to 7.8%. The content of RS in neutral sulfite lye after acid hydrolysis increases by an average of 3 times, while after enzymatic hydrolysis - a maximum of 2 times. It was found by gas-liquid chromatography that pentoses predominate in the obtained hydrolysates. Microbiological processing of media with a similar carbohydrate composition is possible by a number of strains of microorganisms capable of assimilating pentoses, for example, yeast-like fungi of the Saccharomycetaceae family and bacteria of the Enterobacteriaceae family.

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Microwave-assisted enzymatic hydrolysis of starch

10.3390/ecsoc-13-00159 ◽

2009 ◽

Cited By ~ 1

Author(s):

Marcin Lukasiewicz ◽

Anna Osowiec ◽

Magdalena Marciniak

Keyword(s):

Enzymatic Hydrolysis ◽

Microwave Assisted ◽

Hydrolysis Of

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Analyses of enzymatic hydrolysis of bagasse cane of sugar for obtention of ethanol employing a cocktail of natives commercials enzymes.

10.3390/mol2net-04-05300 ◽

2018 ◽

Author(s):

Ángel Batallas ◽

Erenio González ◽

Carmen Salvador ◽

Jonathan Villavicencio ◽

Humberto González Gavilánez ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Hydrolysis Of

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Faculty Opinions recommendation of Enzymatic hydrolysis of pneumococcal capsular polysaccharide renders the bacterium vulnerable to host defense.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.733387483.793547056 ◽

2018 ◽

Author(s):

Mattias Collin

Keyword(s):

Enzymatic Hydrolysis ◽

Host Defense ◽

Capsular Polysaccharide ◽

Hydrolysis Of

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Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

Current Nanoscience ◽

10.2174/1573413714666180611081759 ◽

2019 ◽

Vol 15 (3) ◽

pp. 296-303 ◽

Cited By ~ 5

Author(s):

Swapnil Gaikwad ◽

Avinash P. Ingle ◽

Silvio Silverio da Silva ◽

Mahendra Rai

Keyword(s):

Catalytic Activity ◽

Enzymatic Hydrolysis ◽

Immobilized Enzyme ◽

Free Enzyme ◽

Magnetic Nanomaterials ◽

Sugar Production ◽

Cellulase Enzyme ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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