Growth control in the Drosophila wing disk

2020 ◽  
Vol 12 (3) ◽  
pp. e1478 ◽  
Author(s):  
Jia Gou ◽  
Jay A. Stotsky ◽  
Hans G. Othmer
Keyword(s):  
Growth Control ◽  
Drosophila Wing ◽  
Wing Disk

Quantitative EM of gap junction distribution in mutant drosophila wing discs

1983 ◽  
Vol 41 ◽  
pp. 720-721
Author(s):  
J.S. Ryerse
Keyword(s):  
Gap Junctions ◽  
Growth Control ◽  
Intercellular Junctions ◽  
Wing Disc ◽  
En Bloc ◽  
Metabolic Cooperation ◽  
Drosophila Wing ◽  
Adult Wing ◽  
Wing Discs ◽  
Wing Pouch

Gap junctions are intercellular junctions found in both vertebrates and invertebrates through which ions and small molecules can pass. Their distribution in tissues could be of critical importance for ionic coupling or metabolic cooperation between cells or for regulating the intracellular movement of growth control and pattern formation factors. Studies of the distribution of gap junctions in mutants which develop abnormally may shed light upon their role in normal development. I report here the distribution of gap junctions in the wing pouch of 3 Drosophila wing disc mutants, vg (vestigial) a cell death mutant, 1(2)gd (lethal giant disc) a pattern abnormality mutant and 1(2)gl (lethal giant larva) a neoplastic mutant and compare these with wildtype wing discs.The wing pouch (the anlagen of the adult wing blade) of a wild-type wing disc is shown in Fig. 1 and consists of columnar cells (Fig. 5) joined by gap junctions (Fig. 6). 14000x EMs of conventionally processed, UA en bloc stained, longitudinally sectioned wing pouches were enlarged to 45000x with a projector and tracings were made on which the lateral plasma membrane (LPM) and gap junctions were marked.

Nubbin encodes a POU-domain protein required for proximal-distal patterning in the Drosophila wing

Development ◽  
1995 ◽  
Vol 121 (2) ◽  
pp. 589-599 ◽  
Author(s):  
M. Ng ◽  
F.J. Diaz-Benjumea ◽  
S.M. Cohen
Keyword(s):  
Growth Control ◽  
Normal Growth ◽  
Control Center ◽  
Genetic Mosaics ◽  
Drosophila Wing ◽  
Pou Domain ◽  
Compartment Boundary ◽  
Wing Structures ◽  
Wing Imaginal Discs ◽  
Anterior Posterior

The nubbin gene is required for normal growth and patterning of the wing in Drosophila. We report here that nubbin encodes a member of the POU family of transcription factors. Regulatory mutants which selectively remove nubbin expression from wing imaginal discs lead to loss of wing structures. Although nubbin is expressed throughout the wing primordium, analysis of genetic mosaics suggests a localized requirement for nubbin activity in the wing hinge. These observations suggest the existence of a novel proximal-distal growth control center in the wing hinge, which is required in addition to the well characterized anterior-posterior and dorsal-ventral compartment boundary organizing centers.

Decapentaplegic and growth control in the developing Drosophila wing

Nature ◽  
2015 ◽  
Vol 527 (7578) ◽  
pp. 375-378 ◽  
Author(s):  
Takuya Akiyama ◽  
Matthew C. Gibson
Keyword(s):  
Growth Control ◽  
Drosophila Wing

scholarly journals Interplay between cell proliferation and recruitment controls the duration of growth and final size of the Drosophila wing

2021 ◽  
Author(s):  
Elizabeth Diaz-Torres ◽  
Luis Manuel Muñoz-Nava ◽  
Marcos Nahmad
Keyword(s):  
Cell Proliferation ◽  
Growth Control ◽  
Control Mechanisms ◽  
Final Size ◽  
Exponential Process ◽  
Drosophila Cell ◽  
Cell Recruitment ◽  
Drosophila Wing ◽  
Long Time ◽  
Cell Growth And Proliferation

How organs robustly attain a final size despite perturbations in cell growth and proliferation rates is a fundamental question in developmental biology. Since organ growth is an exponential process driven mainly by cell proliferation, even small variations in cell proliferation rates, when integrated over a relatively long time, will lead to large differences in size, unless intrinsic control mechanisms compensate for these variations. Here we use a mathematical model to consider the hypothesis that in the developing wing of Drosophila, cell recruitment, a process in which undifferentiated neighboring cells are incorporated into the wing primordium, determines the time in which growth is arrested in this system. Under this assumption, our model shows that perturbations in proliferation rates of wing-committed cells are compensated by an inversely proportional duration of growth. This mechanism ensures that the final size of the wing is robust in a range of cell proliferation rates. Furthermore, we predict that growth control is lost when fluctuations in cell proliferation affects both wing-committed and recruitable cells. Our model suggests that cell recruitment may act as a temporal controller of growth to buffer fluctuations in cell proliferation rates, offering a solution to a long-standing problem in the field.

scholarly journals The Myopic-Ubpy-Hrs nexus enables endosomal recycling of Frizzled

2015 ◽  
Vol 26 (18) ◽  
pp. 3329-3342 ◽  
Author(s):  
Tirthadipa Pradhan-Sundd ◽  
Esther M. Verheyen
Keyword(s):  
Wnt Signaling ◽  
Transmembrane Protein ◽  
Strong Association ◽  
Proximity Ligation Assay ◽  
Endosomal Trafficking ◽  
Proximity Ligation ◽  
Drosophila Wing ◽  
Wing Disk ◽  
Molecular Events ◽  
Endosomal Recycling

Endosomal trafficking of signaling proteins plays an essential role in cellular homeostasis. The seven-pass transmembrane protein Frizzled (Fz) is a critical component of Wnt signaling. Although Wnt signaling is proposed to be regulated by endosomal trafficking of Fz, the molecular events that enable this regulation are not completely understood. Here we show that the endosomal protein Myopic (Mop) regulates Fz trafficking in the Drosophila wing disk by inhibiting the ubiquitination and degradation of Hrs. Deletion of Mop or Hrs results in endosomal accumulation of Fz and therefore reduced Wnt signaling. The in situ proximity ligation assay revealed a strong association between Mop and Hrs in the Drosophila wing disk. Overexpression of Hrs rescues the trafficking defect caused by mop knockdown. Mop aids in the maintenance of Ubpy, which deubiquitinates (and thus stabilizes) Hrs. In the absence of the ubiquitin ligase Cbl, Mop is dispensable. These findings support a previously unknown role for Mop in endosomal trafficking of Fz in Wnt-receiving cells.

scholarly journals Dispatched mediates Hedgehog basolateral release to form the long-range morphogenetic gradient in the Drosophila wing disk epithelium

2011 ◽  
Vol 108 (31) ◽  
pp. 12591-12598 ◽  
Author(s):  
A. Callejo ◽  
A. Bilioni ◽  
E. Mollica ◽  
N. Gorfinkiel ◽  
G. Andres ◽  
...  
Keyword(s):  
Long Range ◽  
Drosophila Wing ◽  
Wing Disk ◽  
Morphogenetic Gradient

scholarly journals Dpp from the anterior stripe of cells is crucial for the growth of the Drosophila wing disc

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Shinya Matsuda ◽  
Markus Affolter
Keyword(s):  
Null Allele ◽  
Growth Control ◽  
Wing Disc ◽  
The Other ◽  
Morphogen Gradient ◽  
Posterior Compartment ◽  
Time Points ◽  
Larval Stages ◽  
Drosophila Wing ◽  
Control Growth

The Dpp morphogen gradient derived from the anterior stripe of cells is thought to control growth and patterning of the Drosophila wing disc. However, the spatial-temporal requirement of dpp for growth and patterning remained largely unknown. Recently, two studies re-addressed this question. By generating a conditional null allele, one study proposed that the dpp stripe is critical for patterning but not for growth (Akiyama and Gibson, 2015). In contrast, using a membrane-anchored nanobody to trap Dpp, the other study proposed that Dpp dispersal from the stripe is required for patterning and also for medial wing disc growth, at least in the posterior compartment (Harmansa et al., 2015). Thus, growth control by the Dpp morphogen gradient remains under debate. Here, by removing dpp from the stripe at different time points, we show that the dpp stripe source is indeed required for wing disc growth, also during third instar larval stages.

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