EXPRESSION OF BAX IN CELL NUCLEUS AFTER EXPERIMENTALLY INDUCED APOPTOSIS REVEALED BY IMMUNOGOLD AND EMBEDMENT-FREE ELECTRON MICROSCOPY

2001 ◽  
Vol 25 (8) ◽  
pp. 725-733 ◽  
Author(s):  
B Gajkowska
Keyword(s):  
Electron Microscopy ◽  
Cell Nucleus ◽  
Free Electron ◽  
Induced Apoptosis ◽  
Experimentally Induced

scholarly journals Effects of the cationic protein poly-L-arginine on airway epithelial cellsin vitro

2002 ◽  
Vol 11 (3) ◽  
pp. 141-148 ◽  
Author(s):  
Shahida Shahana ◽  
Caroline Kampf ◽  
Godfried M. Roomans
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Cell Membrane ◽  
Cell Damage ◽  
Membrane Damage ◽  
Light And Electron Microscopy ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Cationic Protein ◽  
Induced Apoptosis

Background: Allergic asthma is associated with an increased number of eosinophils in the airway wall. Eosinophils secrete cationic proteins, particularly major basic protein (MBP).Aim: To investigate the effect of synthetic cationic polypeptides such as poly-L-arginine, which can mimic the effect of MBP, on airway epithelial cells.Methods: Cultured airway epithelial cells were exposed to poly-L-arginine, and effects were determined by light and electron microscopy.Results: Poly-L-arginine induced apoptosis and necrosis. Transmission electron microscopy showed mitochondrial damage and changes in the nucleus. The tight junctions were damaged, as evidenced by penetration of lanthanum. Scanning electron microscopy showed a damaged cell membrane with many pores. Microanalysis showed a significant decrease in the cellular content of magnesium, phosphorus, sodium, potassium and chlorine, and an increase in calcium. Plakoglobin immunoreactivity in the cell membrane was decreased, indicating a decrease in the number of desmosomes.Conclusions: The results point to poly-L-arginine induced membrane damage, resulting in increased permeability, loss of cell-cell contacts and generalized cell damage.

scholarly journals Coherent transition radiation from attosecond electron pulses

2019 ◽  
Vol 205 ◽  
pp. 02018
Author(s):  
Maxim Tsarev ◽  
Peter Baum
Keyword(s):  
Electron Microscopy ◽  
Free Electron ◽  
Transition Radiation ◽  
Beam Diameter ◽  
Pump Probe ◽  
Coherent Transition Radiation ◽  
Electron Pulses ◽  
Velocity Mismatch

We show theoretically and by simulations how coherent transition radiation from tilted surfaces can be used for characterization of attosecond free-electron pulses such as used for pump-probe electron microscopy and diffraction. The tilted geometries eliminate velocity-mismatch and beam-diameter effects, providing sensitivity to attosecond times even for almost arbitrarily large beam diameters.

Transmission and scanning electron microscopy of experimentally induced thyroid tumors in rats

1976 ◽  
Vol 12 (6) ◽  
pp. 315-325
Author(s):  
K. Christov ◽  
T. Sugihara ◽  
R. Lindenfelser ◽  
P. Haubert ◽  
C. Thomas ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Thyroid Tumors ◽  
Scanning Electron ◽  
Experimentally Induced

scholarly journals Detection of early neoplastic changes in experimentally induced colorectal cancer using scanning electron microscopy and cell kinetic studies.

Gut ◽  
1984 ◽  
Vol 25 (7) ◽  
pp. 748-755 ◽  
Author(s):  
T Cooke ◽  
N Kirkham ◽  
D H Stainthorp ◽  
C Inman ◽  
N Goeting ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Kinetic Studies ◽  
Cell Kinetic ◽  
Scanning Electron ◽  
Experimentally Induced

High voltage electron microscopy of cell nucleus fractions: feasibility studies

1981 ◽  
Vol 122 (2) ◽  
pp. 173-180
Author(s):  
C. A. Bourgeois ◽  
J. Zalta ◽  
J. C. Lacaze ◽  
G. Zanchi ◽  
Y. Kihn ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Cell Nucleus ◽  
Feasibility Studies ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
High Voltage Electron

Tomography of the cell nucleus using confocal microscopy and medium voltage electron microscopy

2009 ◽  
Vol 69 (2) ◽  
pp. 127-143 ◽  
Author(s):  
Pavel Tchélidzé ◽  
Aurore Chatron-Colliet ◽  
M. Thiry ◽  
Natahlie Lalun ◽  
Hélène Bobichon ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Confocal Microscopy ◽  
Cell Nucleus ◽  
Medium Voltage ◽  
Voltage Electron

Inelastic Scattering and Electron Spectroscopy

1977 ◽  
Vol 35 ◽  
pp. 692-695
Author(s):  
B. Jouffrey
Keyword(s):  
Electron Microscopy ◽  
Inelastic Scattering ◽  
Free Electron ◽  
Electron Spectroscopy ◽  
Energy Losses ◽  
Scattering Angle ◽  
Crystalline Materials ◽  
Inelastic Event ◽  
Contrast Preservation ◽  
Electron Excitations

The reasons for studying energy losses can be quite different. The motivations are often highly diversified even if searchers are electron microscopists For instance, the problem of useful penetration involves studying the question of chromatic blurring even if in many cases this effect is not sufficient for explaining the limitations in penetration. The formation of damage (direct knock-on and ionization) is interesting in many ways. In electron microscopy an important point is also the contrast preservation in an inelastic event. This point is related to the useful penetratior (principally in crystalline materials). If that is rather well understood in the case of potentials which are not localized (1) (plasmons and single quasi free electron excitations), it is not so clear in the case of localized interactions as electron phonon ones (2,3,4) or inner shell excitations even if the scattering angle is quite small. However the understanding of this contrast preservation is fundamental.

Aspects of the development of yolk spheres in the hen's oöcyte, studied by electron microscopy

Development ◽  
1967 ◽  
Vol 17 (2) ◽  
pp. 267-281
Author(s):  
Ruth Bellairs
Keyword(s):  
Electron Microscopy ◽  
Cell Nucleus ◽  
Raw Materials ◽  
Morphological Changes ◽  
Laying Hen ◽  
Follicle Cells ◽  
Balbiani Body ◽  
Hen’S Egg ◽  
Pass Through ◽  
Yolk Nucleus

The yolk of the hen's egg is composed mainly of proteins, lipids and water (see reviews by Bellairs, 1964; Williams, 1966). It consists essentially of yolk spheres floating in an aqueous protein medium (Grodziński, 1939; Bellairs, 1961). The raw materials from which the yolk is formed are synthesized in the liver of the laying hen and pass from there in the blood to the ovary (see reviews by Romanoff, 1960; Bellairs, 1964). Each oöcyte is enclosed in a capsule of follicle cells, and all the raw materials pass through this capsule before they enter the oöcyte. The morphological changes that take place within the oöcyte as the yolk spheres form have been described previously by light microscopists who have produced a variety of theories to explain their observations. Formerly, it was supposed that yolk arose in the so-called ‘yolk nucleus’, or Balbiani body, which lies alongside the cell nucleus, but few would subscribe to this theory now.

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