Induction of Trypanosoma cruzi Metacyclogenesis in the Gut of the Hematophagous Insect Vector, Rhodnius prolixus, by Hemoglobin and Peptides Carrying αD-Globin Sequences

1995 ◽  
Vol 81 (3) ◽  
pp. 255-261 ◽  
Author(s):  
E.S. Garcia ◽  
M.S. Gonzalez ◽  
P. Deazambuja ◽  
F.E. Baralle ◽  
D. Fraidenraich ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Rhodnius Prolixus ◽  
Insect Vector ◽  
Hematophagous Insect

Serotonin triggers cAMP and PKA-mediated intracellular calcium waves in Malpighian tubules of Rhodnius prolixus

2014 ◽  
Vol 307 (7) ◽  
pp. R828-R836 ◽  
Author(s):  
Paula Gioino ◽  
Brendan G. Murray ◽  
Juan P. Ianowski
Keyword(s):  
Second Messenger ◽  
Fluid Secretion ◽  
Rhodnius Prolixus ◽  
Malpighian Tubules ◽  
Insect Vector ◽  
Excess Water ◽  
Intracellular Ca ◽  
Single Meal ◽  
Hematophagous Insect

Rhodnius prolixus is a hematophagous insect vector of Chagas disease capable of ingesting up to 10 times its unfed body weight in blood in a single meal. The excess water and ions ingested with the meal are expelled through a rapid postprandial diuresis driven by the Malpighian tubules. Diuresis is triggered by at least two diuretic hormones, a CRF-related peptide and serotonin, which were traditionally believed to trigger cAMP as an intracellular second messenger. Recently, calcium has been suggested to act as a second messenger in serotonin-stimulated Malpighian tubules. Thus, we tested the role of calcium in serotonin-stimulated Malpighian tubules from R. prolixus. Our results show that serotonin triggers cAMP-mediated intracellular Ca2+ waves that were blocked by incubation in Ca2+-free saline containing the cell membrane-permeant Ca2+ chelator BAPTA-AM, or the PKA blocker H-89. Treatment with 8-Br-cAMP triggered Ca2+ waves that were blocked by H-89 and BAPTA-AM. Analysis of the secreted fluid in BAPTA-AM-treated tubules showed a 75% reduction in fluid secretion rate with increased K+ concentration, reduced Na+ concentration. Taken together, the results indicate that serotonin triggers cAMP and PKA-mediated Ca2+ waves that are required for maximal ion transport rate.

Trypanosoma cruzi heparin-binding proteins mediate the adherence of epimastigotes to the midgut epithelial cells of Rhodnius prolixus

Parasitology ◽  
2012 ◽  
Vol 139 (6) ◽  
pp. 735-743 ◽  
Author(s):  
F. O. R. OLIVEIRA ◽  
C. R. ALVES ◽  
F. SOUZA-SILVA ◽  
C. M. CALVET ◽  
L. M. C. CÔRTES ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Trypanosoma Cruzi ◽  
Mammalian Cells ◽  
Binding Proteins ◽  
Rhodnius Prolixus ◽  
Insect Vector ◽  
Heparin Binding ◽  
Molecular Masses ◽  
Pre Treatment

SUMMARYHeparin-binding proteins (HBPs) have been demonstrated in both infective forms of Trypanosoma cruzi and are involved in the recognition and invasion of mammalian cells. In this study, we evaluated the potential biological function of these proteins during the parasite-vector interaction. HBPs, with molecular masses of 65·8 kDa and 59 kDa, were isolated from epimastigotes by heparin affinity chromatography and identified by biotin-conjugated sulfated glycosaminoglycans (GAGs). Surface plasmon resonance biosensor analysis demonstrated stable receptor-ligand binding based on the association and dissociation values. Pre-incubation of epimastigotes with GAGs led to an inhibition of parasite binding to immobilized heparin. Competition assays were performed to evaluate the role of the HBP-GAG interaction in the recognition and adhesion of epimastigotes to midgut epithelial cells of Rhodnius prolixus. Epithelial cells pre-incubated with HBPs yielded a 3·8-fold inhibition in the adhesion of epimastigotes. The pre-treatment of epimastigotes with heparin, heparan sulfate and chondroitin sulfate significantly inhibited parasite adhesion to midgut epithelial cells, which was confirmed by scanning electron microscopy. We provide evidence that heparin-binding proteins are found on the surface of T. cruzi epimastigotes and demonstrate their key role in the recognition of sulfated GAGs on the surface of midgut epithelial cells of the insect vector.

Neolignans inhibit Trypanosoma cruzi infection of its triatomine insect vector, Rhodnius prolixus

1999 ◽  
Vol 85 (3) ◽  
pp. 184-187 ◽  
Author(s):  
Marise M. O. Cabral ◽  
Patrícia Azambuja ◽  
Otto R. Gottlieb ◽  
Eloi S. Garcia
Keyword(s):  
Trypanosoma Cruzi ◽  
Rhodnius Prolixus ◽  
Insect Vector ◽  
Cruzi Infection

scholarly journals Towards an understanding of the interactions of Trypanosoma cruzi and Trypanosoma rangeli within the reduviid insect host Rhodnius prolixus

2005 ◽  
Vol 77 (3) ◽  
pp. 397-404 ◽  
Author(s):  
Patrícia Azambuja ◽  
Norman A. Ratcliffe ◽  
Eloi S. Garcia
Keyword(s):  
Trypanosoma Cruzi ◽  
Developmental Stages ◽  
Rhodnius Prolixus ◽  
Parasite Development ◽  
Insect Vector ◽  
Defense System ◽  
Parasite Strain ◽  
Trypanosoma Rangeli ◽  
Physiological Processes ◽  
Prophenoloxidase System

This review outlines aspects on the developmental stages of Trypanosoma cruzi and Trypanosoma rangeli in the invertebrate host, Rhodnius prolixus. Special attention is given to the interactions of these parasites with gut and hemolymph molecules and the effects of the organization of midgut epithelial cells on the parasite development. The vector insect's permissiveness to T. cruzi, which develops in the vector gut, largely depends on the host nutritional state, the parasite strain and the molecular interactions with trypanolytic compounds, lectins and resident bacteria in the gut. T. rangeli invades the hemocoel and once in the hemolymph, can be recognized and activates the defense system of its insect vector, i.e., the prophenoloxidase system, phagocytosis, hemocyte microaggregation, superoxide and nitric oxide activity and the eicosanoid biosynthesis pathway. Taken together, these findings not only provide a better understanding of the interactions parasite - insect vector, but also offer new insights into basic physiological processes involved in the parasites transmission.

scholarly journals Participation of Trypanosoma cruzi gp63 molecules on the interaction with Rhodnius prolixus

Parasitology ◽  
2019 ◽  
Vol 146 (8) ◽  
pp. 1075-1082 ◽  
Author(s):  
Karina M. Rebello ◽  
Livia A. Uehara ◽  
Vítor Ennes-Vidal ◽  
Aline S. Garcia-Gomes ◽  
Constança Britto ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Rhodnius Prolixus ◽  
Mammalian Host ◽  
Insect Vector ◽  
Metal Chelators ◽  
Insect Midgut ◽  
Host Interaction ◽  
Vector Interaction ◽  
Vector Borne ◽  
First Time

AbstractTrypanosoma cruzi is the causative agent of Chagas disease, a vector-borne disease. The parasite molecules involved in vector interaction have been little investigated. Metallopeptidases and gp63 molecules have been implicated in parasite adhesion of several trypanosomatids to the insect midgut. Although gp63 homologues are highly expanded in the T. cruzi genome, and are implicated in parasite–mammalian host interaction, its role in the insect vector has never been explored. Here, we showed that divalent metal chelators or anti-Tcgp63-I antibodies impaired T. cruzi adhesion to Rhodnius prolixus midgut. Parasites isolated after insect colonization presented a drastic enhancement in the expression of Tcgp63-I. These data highlight, for the first time, that Tcgp63-I and Zn-dependent enzymes contribute to the interaction of T. cruzi with the insect vector.

Expression of GP82 and GP90 surface glycoprotein genes of Trypanosoma cruzi during in vivo metacyclogenesis in the insect vector Rhodnius prolixus

Acta Tropica ◽  
2008 ◽  
Vol 105 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Esteban M. Cordero ◽  
Luciana G. Gentil ◽  
Gladys Crisante ◽  
José Luis Ramírez ◽  
Nobuko Yoshida ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Rhodnius Prolixus ◽  
Surface Glycoprotein ◽  
Insect Vector

Antiserum against perimicrovillar membranes and midgut tissue reduces the development of Trypanosoma cruzi in the insect vector, Rhodnius prolixus

2006 ◽  
Vol 114 (4) ◽  
pp. 297-304 ◽  
Author(s):  
M.S. Gonzalez ◽  
A. Hamedi ◽  
J.M. Albuquerque-Cunha ◽  
N.F.S. Nogueira ◽  
W. De Souza ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Rhodnius Prolixus ◽  
Insect Vector

Colonization of Rhodnius prolixus gut by Trypanosoma cruzi involves an extensive parasite killing

Parasitology ◽  
2016 ◽  
Vol 143 (4) ◽  
pp. 434-443 ◽  
Author(s):  
ROBERTA CARVALHO FERREIRA ◽  
RAFAEL LUIS KESSLER ◽  
MARCELO GUSTAVO LORENZO ◽  
RAFAELA MAGALHÃES MACEDO PAIM ◽  
LUCIANA DE LIMA FERREIRA ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Population Size ◽  
Chagas Disease ◽  
Intestinal Tract ◽  
Parasite Load ◽  
Rhodnius Prolixus ◽  
Insect Vector ◽  
Posterior Midgut ◽  
Development And Differentiation ◽  
Anterior Midgut

SUMMARYTrypanosoma cruzi, the etiological agent of Chagas disease, is ingested by triatomines during their bloodmeal on an infected mammal. Aiming to investigate the development and differentiation of T. cruzi inside the intestinal tract of Rhodnius prolixus at the beginning of infection we fed insects with cultured epimastigotes and blood trypomastigotes from infected mice to determine the amount of recovered parasites after ingestion. Approximately 20% of the ingested parasites was found in the insect anterior midgut (AM) 3 h after feeding. Interestingly, a significant reduction (80%) in the numbers of trypomastigotes was observed after 24 h of infection suggesting that parasites were killed in the AM. Moreover, few parasites were found in that intestinal portion after 96 h of infection. The evaluation of the numbers of parasites in the posterior midgut (PM) at the same periods showed a reduced parasite load, indicating that parasites were not moving from the AM. Additionally, incubation of blood trypomastigotes with extracts from R. prolixus AMs revealed that components of this tissue could induce significant death of T. cruzi. Finally, we observed that differentiation from trypomastigotes to epimastigotes is not completed in the AM; instead we suggest that trypomastigotes change to intermediary forms before their migration to the PM, where differentiation to epimastigotes takes place. The present work clarifies controversial points concerning T. cruzi development in insect vector, showing that parasite suffers a drastic decrease in population size before epimastigonesis accomplishment in PM.

Memoirs: A Study of the Mitochondria of Trypanosomes

1940 ◽  
Vol s2-82 (326) ◽  
pp. 261-266
Author(s):  
ROBERT M. WOTTON
Keyword(s):  
Trypanosoma Cruzi ◽  
Growth Stages ◽  
Insect Vector ◽  
Morphological Variations ◽  
Trypanosoma Lewisi ◽  
Melophagus Ovinus ◽  
Trypanosoma Equiperdum

Mitochondria have been demonstrated in the adult forms of the following species of trypanosomes: Trypanosoma lewisi, Trypanosoma duttoni, Trypanosoma cruzi, and Trypanosoma equiperdum. They have been shown also in the crithidial forms of Trypanosoma cruzi from culture, and of Trypanosoma melophagium from the insect vector, Melophagus ovinus. No essential morphological variations in the mitochondria among the five species of trypanosomes studied, nor in those among the several growth stages, were observed.

scholarly journals Learning Spatial Aversion Is Sensory-Specific in the Hematophagous Insect Rhodnius prolixus

2018 ◽  
Vol 9 ◽  
Author(s):  
Sebastian Minoli ◽  
Agustina Cano ◽  
Gina Pontes ◽  
Amorina Magallanes ◽  
Nahuel Roldán ◽  
...  
Keyword(s):  
Rhodnius Prolixus ◽  
Hematophagous Insect
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