Site-Specific Quality Management in Wheat Results from the 2003 Field Trials in Argentina

Author(s):  
R. Bongiovanni ◽  
A. Méndez ◽  
W. Robledo ◽  
M. Bragachini ◽  
F. Proietti ◽  
...  
2017 ◽  
Vol 8 (2) ◽  
pp. 450-454
Author(s):  
Y. Reckleben ◽  
T. Grau ◽  
S. Schulz ◽  
H. G. Trumpf

Site-specific management provides the ability to align the production intensity to demand and thus adjust the expenses to the necessary level. So it is possible to increase the proportion of marketable commodity in the normal sort–size of 40 mm to 60 mm. Planting distances adapted to the soil properties seem to achieve this objective. It is possible to further optimize the proportion of marketable commodity especially in the potato regions where irrigation and fertilization already contribute to a consistently high yield. Different planting distances on the soil sites by EM38 were tested in field trials. Planting distances of 31.50 cm in the row on the light (sandy) soil, 24.50 cm on middle and 27.50 cm on the heavy soil sites seems the best for these three years. There is a yield impact in total, as well as in the proportion of marketable commodity. Depending on the planting strategy, increases in income up to €153 per hectare can be obtained.


Author(s):  
Peter Kusstatscher ◽  
Wisnu Adi Wicaksono ◽  
Dhivya P. Thenappan ◽  
Eveline Adam ◽  
Henry Müller ◽  
...  

The targeted application of plant growth promoting rhizobacteria (PGPR) provides the key for a future sustainable agriculture with reduced pesticide application. PGPR interaction with the indigenous microbiota is poorly understood but essential to develop reliable applications. Therefore, Stenotrophomonas rhizophila SPA-P69 was applied as seed coating and in combination with a fungicide based on the active ingredients fludioxonil, metalaxyl-M, captan and ziram. Plant performance and rhizosphere composition of treated and non-treated maize plants of two field trials were analyzed. Plant health was significantly increased by treatment; however overall corn yield was not changed. By applying high-throughput amplicon sequencing of the 16S rRNA and the ITS genes, the bacterial and fungal changes in the rhizosphere due to different treatments were determined. Despite treatments had a significant impact on the rhizosphere microbiota (9- 12%), the field site was identified as main driver (27- 37%). Soil microbiota composition from each site was significantly different, which explains the site-specific effects. In this study we were able to show first indications how PGPR treatments increase plant health via microbiome shifts in a site-specific manner. This way first steps towards a detailed understanding of PGPRs and developments of consistently efficient applications in diverse environments are set.


2020 ◽  
Vol 8 (10) ◽  
pp. 1506
Author(s):  
Peter Kusstatscher ◽  
Wisnu Adi Wicaksono ◽  
Dhivya P. Thenappan ◽  
Eveline Adam ◽  
Henry Müller ◽  
...  

The targeted application of plant growth-promoting rhizobacteria (PGPR) provides the key for a future sustainable agriculture with reduced pesticide application. PGPR interaction with the indigenous microbiota is poorly understood, but essential to develop reliable applications. Therefore, Stenotrophomonas rhizophila SPA-P69 was applied as a seed coating and in combination with a fungicide based on the active ingredients fludioxonil, metalaxyl-M, captan and ziram. The plant performances and rhizosphere compositions of treated and non-treated maize plants of two field trials were analyzed. Plant health was significantly increased by treatment; however, overall corn yield was not changed. By applying high-throughput amplicon sequencing of the 16S rRNA and the ITS genes, the bacterial and fungal changes in the rhizosphere due to different treatments were determined. Despite the fact that treatments had a significant impact on the rhizosphere microbiota (9–12%), the field site was identified as the main driver (27–37%). The soil microbiota composition from each site was significantly different, which explains the site-specific effects. In this study we were able to show the first indications how PGPR treatments increase plant health via microbiome shifts in a site-specific manner. This way, first steps towards a detailed understanding of PGPRs and developments of consistently efficient applications in diverse environments are made.


2001 ◽  
Vol 41 (5) ◽  
pp. 655 ◽  
Author(s):  
M. T. F. Wong ◽  
R. J. Corner ◽  
S. E. Cook

The intensely weathered nature of Western Australian cropping soils and the long history of potassium depletion by the farming system has resulted in increased incidence of potassium deficiency in wheat. There is currently no scientifically based method for potassium recommendation in Western Australia. This paper describes the use of site-specific plot-scale field trials carried out in 1995–98 and a crop response model to develop a generally applicable potassium recommendation system. Geographic information system technology was used to extend the uniform potassium recommendation system into a system for mapping spatially variable potassium requirement that takes account of crop demand and soil available potassium. The field trials were carried out on a range of soil types and showed that wheat response to potassium can be described by the Mitscherlich equation. The size of the response was dependent on the soil test value for plant available potassium and the yield of the crop. The latter is mainly dependent on rainfall in the water-limited Mediterranean environment of Western Australia. The relationships between the maximum achievable yield, crop response and soil available potassium values were quantified in order to allow the decision support system to be developed for uniform whole-paddock fertiliser recommendation. Both soil available potassium and yield are very spatially variable in Western Australia and for wheat, the coefficient of variation of yield within the paddock is often of the order of 30%. Soil property variation can be of a similar order. Maps of soil available potassium values and of spatially variable target yield determined either from (i) farmer’s estimate, (ii) yield monitors and (iii) remotely sensed normalised difference vegetation index measurements allow this decision system to map spatially variable potassium requirement. Comparison of the map of potassium requirement with measured spatially variable response to potassium showed that the decision support system performed satisfactorily.


Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.


2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.


2006 ◽  
Vol 37 (6) ◽  
pp. 49
Author(s):  
Bruce Jancin
Keyword(s):  

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