Isolation and Characterization of a Stem Cell Side-Population from Mouse Hair Follicles

2013 ◽  
pp. 259-268 ◽  
Author(s):  
Paula L. Miliani de Marval ◽  
Sun Hye Kim ◽  
Marcelo L. Rodriguez-Puebla
Keyword(s):  
Stem Cell ◽  
Side Population ◽  
Hair Follicles ◽  
Isolation And Characterization

Isolation and Characterization of a Fetal-Maternal Microchimeric Stem Cell Population in Maternal Hair Follicles Long after Parturition

2019 ◽  
Vol 15 (4) ◽  
pp. 519-529 ◽  
Author(s):  
Cosmin Andrei Cismaru ◽  
Olga Soritau ◽  
Ancuta - Maria Jurj ◽  
Raduly Lajos ◽  
Bogdan Pop ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Population ◽  
Hair Follicles ◽  
Stem Cell Population ◽  
Isolation And Characterization

Characterization of stem cell–like cancer cells in immune-competent mice

Blood ◽  
2006 ◽  
Vol 108 (12) ◽  
pp. 3906-3912 ◽  
Author(s):  
Jorg A. Kruger ◽  
Charles D. Kaplan ◽  
Yunping Luo ◽  
He Zhou ◽  
Dorothy Markowitz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Cells ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Side Population ◽  
Carcinoma Cell Lines ◽  
Tumorigenic Potential ◽  
4T1 Cells

AbstractRecently, the cancer stem cell hypothesis has gained significant recognition as the descriptor of tumorigenesis. Although previous studies relied on transplanting human or rat tumor cells into immunecompromised mice, our study used the Hoechst 33342 dye–based side population (SP) technique to isolate and transplant stem cell–like cancer cells (SCLCCs) from the 4T1 and NXS2 murine carcinoma cell lines into the immune-competent microenvironment of syngeneic mice. 4T1 cells displayed an SP of 2% with a Sca-1highc-Kit–CD45– phenotype, whereas NXS2 cells contained an SP of 0.2% with a Sca-1highCD24highc-Kit–CD45–GD high2 phenotype. Reverse transcription–polymerase chain reaction (RT-PCR) further revealed up-regulation in SP cells of ABCG2, Sca-1, Wnt-1, and TGF-β2. Additionally, 4T1 and NXS2 SP cells exhibited increased resistance to chemotherapy, and 4T1 SP cells also showed an increased ability to efflux doxorubicin, which correlated with a selective increase in the percentage of SP cells found in the tumors of doxorubicin-treated mice. Most importantly, SP cells showed a markedly higher repopulation and tumorigenic potential in vivo, which correlated with an increased number of cells in the SP compartment of SP-derived tumors. Taken together, these results show that we successfully characterized SCLCCs from 2 murine carcinoma cell lines in the immune-competent microenvironment of syngeneic mice.

Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies

2017 ◽  
pp. 205-223
Author(s):  
Zezhou Zhao ◽  
Dillon C. Muth ◽  
Vasiliki Mahairaki ◽  
Linzhao Cheng ◽  
Kenneth W. Witwer
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Isolation And Characterization

Isolation and Characterization of Human Epidermal Stem Cells

1996 ◽  
Vol 91 (2) ◽  
pp. 141-146 ◽  
Author(s):  
P. H. Jones
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Matrix Proteins ◽  
Stem Cell Markers ◽  
Epidermal Stem Cells ◽  
Cell Behaviour ◽  
Isolation And Characterization ◽  
Integrin Family

1. The keratinocytes in human epidermis are constantly turned over and replaced by a population of stem cells located in the basal epidermal layer. Until recently there were no markers allowing the isolation of viable epidermal stem cells. However, it has now been shown that epidermal stem cells can be isolated both in vitro and direct from the epidermis as they express high levels of functional β1 integrin family receptors for extracellular matrix proteins. 2. The evidence for integrins as stem cell markers and the insights that have been gained into stem cell behaviour are reviewed.

Isolation and characterization of breast cancer stem cell‐like phenotype by Oct4 promoter‐mediated activity

2020 ◽  
Vol 235 (11) ◽  
pp. 7840-7848 ◽  
Author(s):  
Zahra Ghanei ◽  
Abbas Jamshidizad ◽  
Morteza Daliri Joupari ◽  
Mehdi Shamsara
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Breast Cancer Stem Cell ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Embryonic Stem Cell-Derived Cardiac Purkinje Cells

Stem Cells ◽  
2015 ◽  
Vol 33 (4) ◽  
pp. 1102-1112 ◽  
Author(s):  
Karen Maass ◽  
Akshay Shekhar ◽  
Jia Lu ◽  
Guoxin Kang ◽  
Fiona See ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Purkinje Cells ◽  
Embryonic Stem ◽  
Isolation And Characterization ◽  
Cardiac Purkinje Cells

Isolation and Characterization of Stem Cell Clones from Adult Human Ligament

2009 ◽  
Vol 15 (9) ◽  
pp. 2625-2636 ◽  
Author(s):  
Weerachai Singhatanadgit ◽  
Nikolaos Donos ◽  
Irwin Olsen
Keyword(s):  
Stem Cell ◽  
Adult Human ◽  
Isolation And Characterization ◽  
Cell Clones

Isolation and Characterization of Embryonic Stem Cell-Like Cells From in vitro Produced Goat (Capra hircus) Embryos

2011 ◽  
Vol 22 (4) ◽  
pp. 181-196 ◽  
Author(s):  
Arun Kumar De ◽  
Dhruba Malakar ◽  
Yogesh S. Akshey ◽  
Manoj Kumar Jena ◽  
Rahul Dutta
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Capra Hircus ◽  
Isolation And Characterization
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