Isolation of Actinobacteria from Soil and Marine Sediment Samples

<div> <p>Sediment provenance is of key importance for understanding transport history and characterising sediment source regions in the marine and terrestrial environment. Radiogenic isotopes are widely used to identify inland and coastal sediment origins. They document changes in detrital terrigenous sediment fluxes which can be related to continental hydrological variability. Understanding sediment sources to the ocean is a pre-requisite before interpreting past climate archives in marine sediment cores.</p> </div><div> <p><span>South African coastal drainage basins are composed of various geological units, each reflected by different radiogenic isotope signals in the sediment. In addition to the age and nature of their source rocks, the sediment type influences this radiogenic signature.</span></p> </div><div> <p><span>Here, we present a review of the present-day radiogenic isotopic fingerprints of South African river catchments signals from new river sediment samples with the aim to gain a broad spatial coverage of the source rocks in the region and their relative contributions of terrigenous sediment delivered to the ocean. This information will be applied to marine sediment core MD20-3591 (36&#176; 43.707 S; 22&#176; 9.151 E, water depth 2464m), located offshore South Africa which has the potential to record both Agulhas Current and terrestrial variability. The core site receives a significant amount of terrigenous material from the African continents via riverine input. During the last glacial period, these rivers flowed across the continental shelf within a subdued incised valley. The Gourritz River catchment drains the Cape Supergroup and Karoo Supergroup, typical of these southern drainage basins, whereas the eastern Cape rivers drain the Karoo Supergroup geological unit which is capped by the Drakensberg basalts.</span></p> </div><div> <p><span>We are using the knowledge gained from these new South African terrestrial river sediment samples to identify the sources and transport pathways of the terrigenous sediments in MD20-3591. Of particular interest is the sensitivity of the radiogenic isotopic signatures to grain size variabilities and how this relationship can help to define local or distal sediments. These records will allow us to explore variability in regional hydroclimate in relation to the abundant archaeological evidence of cultural and technological innovations of Middle Stone Age humans in southern Africa.</span></p> </div>

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Flow Injection On-Line Preconcentration and Flame AAS Determination of Copper, Cadmium and Lead in Marine Sediment Samples

Analytical Letters ◽

10.1080/00032719708002316 ◽

1997 ◽

Vol 30 (5) ◽

pp. 1037-1050 ◽

Cited By ~ 11

Author(s):

S. Kartikeyan ◽

B. Vijayalekshmy ◽

S. Chandramouleeswaran ◽

T. P. Rao ◽

C. S. P. Iyer

Keyword(s):

Flow Injection ◽

Marine Sediment ◽

Sediment Samples ◽

On Line ◽

Cadmium And Lead ◽

Determination Of Copper

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Improvements of a combined size exclusion chromatography and solid phase extraction approach for the clean-up of marine sediment samples for trace analysis of pesticides

Fresenius Journal of Analytical Chemistry ◽

10.1007/s002160050481 ◽

1997 ◽

Vol 358 (5) ◽

pp. 630-634 ◽

Cited By ~ 14

Author(s):

K. Bester ◽

Heinrich Hühnerfuss

Keyword(s):

Solid Phase Extraction ◽

Size Exclusion Chromatography ◽

Marine Sediment ◽

Trace Analysis ◽

Solid Phase ◽

Size Exclusion ◽

Phase Extraction ◽

Sediment Samples ◽

Exclusion Chromatography

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MEIOFAUNA (FORAMINIFERA) IN SEDIMENTS AND ITS RELATION TO WHITE SANDY BEACH OF SENGGIGI AND WATER CONDITION OFF WEST LOMBOK

Jurnal Ilmu dan Teknologi Kelautan Tropis ◽

10.29244/jitkt.v4i1.7805 ◽

2012 ◽

Vol 4 (1) ◽

Author(s):

Kresna T. Dewi ◽

L. Arifin ◽

A. Yuningsih ◽

Y. Permanawati

Keyword(s):

Marine Sediment ◽

Benthic Foraminifera ◽

Coastal Area ◽

Sandy Beach ◽

Coral Growth ◽

Small Islands ◽

Offshore Area ◽

Sediment Samples ◽

Water Condition ◽

White Sands

A development of meiofaunal data (foraminifera) from offshore area of West Lombok was conducted on 20 marine sediment samples. The samples were taken out using a grab sampler in 1997. The purpose of this study was to apply the formula of Foraminiferal Index by Hallock et al. (2003). Another purpose of this study was to recognize its relation of white sands of Senggigi beach that was dominated by Shlumbergerella floresiana. The result of analysis showed that the value of Foraminiferal Index (FI) was varies between 1.22 and 9.81. The low value of FI (<2) was found at sites that were close to the coastal area. The values FI >2 and <4 occur at five stations that gave indication of limited environment for coral growth. Value of FI >4 that showed good water condition was found in an area surrounding small islands of Gili Trawangan, Gili Meno, Gili Air and southern part of the study area. Based on the identification of foraminiferal specimens, the occurrence of Shlumbergerella floresiana was not abundant as it was accumulated in Senggigi beach. This result showed that this species did not come from samples studied but might come from marine sediment adjacent to the Senggigi beachKeywords: Benthic foraminifera, FORAM Index, west Lombok

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Penapisan dan Identifikasi Bakteri Penghasil Agarase dari Sampel Sedimen Laut Bara Caddi, Sulawesi Selatan

Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan ◽

10.15578/jpbkp.v16i1.699 ◽

2021 ◽

Vol 16 (1) ◽

Author(s):

Dewi Seswita Zilda ◽

Gintung Patantis ◽

Mada Triandala Sibero ◽

Yusro Nuri Fawzya

Keyword(s):

16S Rrna ◽

16S Rrna Gene ◽

Marine Sediment ◽

Marine Bacteria ◽

Solid Medium ◽

Rrna Gene ◽

Clear Zone ◽

Sediment Samples ◽

South Sulawesi ◽

The 16S Rrna Gene

Agarase adalah enzim yang mampu menghidrolisis agar menjadi oligoagar yang sudah banyak diaplikasikan dalam industri kesehatan dan kosmetik. Bakteri laut merupakan mikroba yang paling banyak dilaporkan sebagai sumber untuk isolasi bakteri penghasil agarase. Penelitian ini bertujuan untuk melakukan penapisan, isolasi, dan identifikasi bakteri penghasil agarase dari sedimen laut. Sampel sedimen diambil dari pantai Pulau Bara Caddi, Sulawesi Selatan. Penapisan dilakukan menggunakan media air laut yang ditambahkan tripton 0,5%, ekstrak ragi 0,1%, dan agar 2%. Identifikasi dilakukan dengan amplifikasi gen 16S rRNA. Sebanyak 45 isolat berhasil dimurnikan, 16 diantaranya merupakan bakteri penghasil agarase. Pola zona bening yang terbentuk terlihat berbeda-beda, hal ini diduga disebabkan oleh perbedaan jenis agarase yang dihasilkan oleh masing-masing isolat. Hasil penelitian menunjukkan bahwa terdapat 4 genera bakteri yang memiliki kemiripan yang tinggi dengan 16 isolat bakteri penghasil agarase yang terdapat pada sampel sedimen yaitu Vibrio, Alteromonas, Salinivibrio, dan Marinobacter. Vibrio merupakan genus yang paling dominan diikuti oleh Alteromonas dan hanya satu isolat yang menunjukkan kesamaan dengan Salinivibrio dan Marinobacter. ABSTRACTAgarase is an enzyme that hydrolyze agar into agaro oligosaccharide which have been applied in health and cosmetic industries. Marine bacteria are the most widely reported microbes as a source for isolation of agarase-producing bacteria. This work was aimed to screen, isolate, and identify the agarase-producing bacteria from marine sediment. The sediment samples were collected from the sea around Bara Caddi Island, South Sulawesi. The screening of agarase-producing bacteria was carried out using seawater media containing 0.5% tryptone, 0.1 % yeast extract with 2 % agar. The identification of the bacteria obtained was carried out by amplification of the 16S rRNA gene. A total of 45 isolates were successfully purified, 16 of which were agarase-producing bacteria. The clear zone formed on solid medium by some isolates showed different pattern which may be caused by the type of agarase produced by each isolate. The results showed that there were 4 genera of bacteria which similar to the 16 isolates agarase-producing bacteria found in sediment samples i.e. Vibrio, Alteromonas, Salinivibrio, and Marinobacter. Vibrio is the most dominant genus followed by Alteromonas and only one isolate showed similarity to Salinivibrio and Marinobacter.

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