Objective: The human vagina and gut are known to harbor a wide range of bacteria. Some useful bacteria maintain the vaginal pH around 3–4.5. This acidity of the vagina helps to inhibit pathogenic organisms that cause urogenital infections. The present study involved screening of previously identified Enterococcus strains of human origin for their probiotic physiological properties.
Materials and Methods: The strains were screened for their biofilm, antibiofilm, antagonistic, antibiotic resistance, adherence, aggregating, and hydrogen peroxide production abilities.
Results: Enterococcus canintestini S26B, Entamoeba dispar S27A, E. dispar S26A, E. dispar S20B, E. canintestini AB2, Enterococcus villorum SB2, and Enterococcus rivorum S22C displayed in vitro probiotic properties.
Conclusions: These strains can be used as probiotic candidates and may prove their potential in human or animal feed only after further clinical studies.