scholarly journals IN VITRO ASSESSMENT OF PHYSIOLOGICAL PROPERTIES OF ENTEROCOCCUS STRAINS OF HUMAN ORIGIN FOR POSSIBLE PROBIOTIC USE

2019 ◽  
pp. 194-203
Author(s):  
ASHLESHA BHAGWAT ◽  
NANDANWAR Y S ◽  
RAHUL WARKE ◽  
UDAY S ANNAPURE
Keyword(s):  
Animal Feed ◽  
Probiotic Properties ◽  
Human Origin ◽  
Entamoeba Dispar ◽  
Urogenital Infections ◽  
Physiological Properties ◽  
Wide Range ◽  
Human Vagina ◽  
In Vitro Assessment

Objective: The human vagina and gut are known to harbor a wide range of bacteria. Some useful bacteria maintain the vaginal pH around 3–4.5. This acidity of the vagina helps to inhibit pathogenic organisms that cause urogenital infections. The present study involved screening of previously identified Enterococcus strains of human origin for their probiotic physiological properties. Materials and Methods: The strains were screened for their biofilm, antibiofilm, antagonistic, antibiotic resistance, adherence, aggregating, and hydrogen peroxide production abilities. Results: Enterococcus canintestini S26B, Entamoeba dispar S27A, E. dispar S26A, E. dispar S20B, E. canintestini AB2, Enterococcus villorum SB2, and Enterococcus rivorum S22C displayed in vitro probiotic properties. Conclusions: These strains can be used as probiotic candidates and may prove their potential in human or animal feed only after further clinical studies.

scholarly journals Amyloid Beta: Multiple Mechanisms of Toxicity and Only Some Protective Effects?

2014 ◽  
Vol 2014 ◽  
pp. 1-15 ◽  
Author(s):  
Paul Carrillo-Mora ◽  
Rogelio Luna ◽  
Laura Colín-Barenque
Keyword(s):  
Amyloid Beta ◽  
Protective Effects ◽  
Protective Mechanisms ◽  
Experimental Conditions ◽  
Mechanisms Of Toxicity ◽  
Mitochondrial Alterations ◽  
Physiological Properties ◽  
Wide Range

Amyloid beta (Aβ) is a peptide of 39–43 amino acids found in large amounts and forming deposits in the brain tissue of patients with Alzheimer’s disease (AD). For this reason, it has been implicated in the pathophysiology of damage observed in this type of dementia. However, the role of Aβin the pathophysiology of AD is not yet precisely understood. Aβhas been experimentally shown to have a wide range of toxic mechanismsin vivoandin vitro, such as excitotoxicity, mitochondrial alterations, synaptic dysfunction, altered calcium homeostasis, oxidative stress, and so forth. In contrast, Aβhas also shown some interesting neuroprotective and physiological properties under certain experimental conditions, suggesting that both physiological and pathological roles of Aβmay depend on several factors. In this paper, we reviewed both toxic and protective mechanisms of Aβto further explore what their potential roles could be in the pathophysiology of AD. The complete understanding of such apparently opposed effects will also be an important guide for the therapeutic efforts coming in the future.

In vitro evaluation of probiotic properties of Lactobacillus species of food and human origin

LWT ◽  
2019 ◽  
Vol 106 ◽  
pp. 201-208 ◽  
Author(s):  
Aarti A. Boricha ◽  
Satyamitra L. Shekh ◽  
Sheetal P. Pithva ◽  
Padma S. Ambalam ◽  
Bharatkumar Rajiv Manuel Vyas
Keyword(s):  
Lactobacillus Species ◽  
Probiotic Properties ◽  
Human Origin ◽  
In Vitro Evaluation

In vitro assessment of probiotic properties of Bacillus isolated from naturally fermented congee from Inner Mongolia of China

2010 ◽  
Vol 26 (8) ◽  
pp. 1369-1377 ◽  
Author(s):  
Yanyan Wang ◽  
Heping Zhang ◽  
Lei Zhang ◽  
Wenjun Liu ◽  
Yong Zhang ◽  
...  
Keyword(s):  
Inner Mongolia ◽  
Probiotic Properties ◽  
In Vitro Assessment

scholarly journals In vitro assessment of metabolic profile of Enterococcus strains of human origin

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Ashlesha Bhagwat ◽  
Uday S. Annapure
Keyword(s):  
Metabolic Profile ◽  
Food Industry ◽  
Radical Scavenging ◽  
Starter Cultures ◽  
Bile Salt Hydrolase ◽  
Human Origin ◽  
Future Potential ◽  
In Vitro Assessment ◽  
Radical Scavenging Properties

Abstract Background In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). Results Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml−1), protease (192–264 mg ml−1), lipase (8–10 mg ml−1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml−1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml−1) and sunflower oil (highest yield 48 μg ml−1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30–50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. Conclusion The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.

In Vitro Probiotic Properties of Lactobacillus salivarius MG242 Isolated from Human Vagina

2017 ◽  
Vol 10 (2) ◽  
pp. 343-349 ◽  
Author(s):  
Chang-Ho Kang ◽  
Seul Hwa Han ◽  
YongGyeong Kim ◽  
Nam-Soo Paek ◽  
Jae-Seong So
Keyword(s):  
Probiotic Properties ◽  
Lactobacillus Salivarius ◽  
Human Vagina

scholarly journals Production of a Potentially Probiotic Product for Animal Feed and Evaluation of Some of Its Probiotic Properties

2021 ◽  
Vol 22 (18) ◽  
pp. 10004
Author(s):  
Rubén Agregán-Pérez ◽  
Elisa Alonso-González ◽  
Juan Carlos Mejuto ◽  
Nelson Pérez-Guerra
Keyword(s):  
Animal Feed ◽  
Low Cost ◽  
Skim Milk ◽  
Acetic Acid Bacteria ◽  
Probiotic Properties ◽  
The European Union ◽  
Gastrointestinal Conditions ◽  
Probiotic Product ◽  
Kefir Grains

Nowadays, probiotics have been proposed for substituting antibiotics in animal feed since the European Union banned the latter compounds in 2006 to avoid serious side effects on human health. Therefore, this work aimed to produce a probiotic product for use in animal feed by fed-batch fermentation of whey with a combination of kefir grains, AGK1, and the fermented whole milk used to activate these kefir grains. The probiotic culture obtained was characterized by high levels of biomass (8.03 g/L), total viability (3.6 × 108 CFU/mL) and antibacterial activity (28.26 Activity Units/mL). Some probiotic properties of the probiotic culture were investigated in vitro, including its survival at low pH values, under simulated gastrointestinal conditions, after freezing in skim milk at −20 °C, and in the commercial feed during storage at room temperature. The viable cells of lactic and acetic acid bacteria and yeasts exhibited higher tolerance to acidic pH and simulated gastrointestinal conditions when the cells were protected with skim milk and piglet feed, compared with washed cells. The results indicated the feasibility of producing a probiotic product at a low cost with a potential application in animal feed.

scholarly journals IN VITRO ASSESSMENT OF POTENTIAL PROBIOTIC MICROORGANISMS FOR APPLICATION IN ANIMAL FEEDING

2018 ◽  
Vol 54 (4A) ◽  
pp. 250 ◽  
Author(s):  
Le Thi Hong Van
Keyword(s):  
Enzyme Production ◽  
Animal Feed ◽  
Digestive Enzyme ◽  
Saccharomyces Boulardii ◽  
Bacillus Sp ◽  
Acid Generation ◽  
Gastrointestinal Conditions ◽  
Animal Feeding ◽  
In Vitro Assessment

This study aimed to evaluate and to select potential probiotic microorganisms. The obtained results would be further studied for application in production of animal feed. A total of 16 strains of microorganisms including 11 strains of Lactobacillus, four strains of Bacillus and a yeast strain Saccharomyces boulardii PLCP were investigated for acid production, digestive enzyme production and antimicrobial activity as well as their survival when exposed to simulated gastrointestinal conditions. The results showed that all 11 strains of Lactobacillus bacteria were capable of acid generation (in the range from 18.05 – 19.04 g/l). All four strains of Bacillus bacteria were capable of producing protease. Only Bacillus sp D7 strain was capable of producing three digestive enzymes (protease, amylase, cellulase) with hydrolyzed hollows ranged from 15.5–18.5 mm. The antibacterial activity of 9/16 test strains was positive against Salmonella Typhimurium, Staphylococcus aureus and Escherichia coli. Survivability of 15 test microorganisms in simulated gastrointestinal conditions was relatively high (c.a. 80 %). Three strains of L. acidophilus VAST, S. boulardii PLCP and Bacillus sp D7 when in mixture demonstrated a great potential as probiotics for animal.

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