Visualization of Neurokinin, Growth Factor, Ion Channel and Second Messenger Binding Sites in the Brain: Clues to their Function

1989 ◽  
pp. 73-85
Author(s):  
Rémi Quirion ◽  
Dalia M. Araujo ◽  
Than Vinh Dam ◽  
Michel Dalpé ◽  
Jean-Guy Chabot
Keyword(s):  
Growth Factor ◽  
Ion Channel ◽  
Binding Sites ◽  
Second Messenger ◽  
The Brain

scholarly journals Retrograde nerve growth factor signaling modulates tooth mechanical hyperalgesia induced by orthodontic tooth movement via acid-sensing ion channel 3

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Meiya Gao ◽  
Xinyu Yan ◽  
Yanzhu Lu ◽  
Linghuan Ren ◽  
Shizhen Zhang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Nerve Growth Factor ◽  
Growth Factor ◽  
Ion Channel ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Neutralizing Antibody ◽  
Mechanical Hyperalgesia ◽  
Nerve Growth ◽  
Retrograde Labeling

AbstractOrthodontic tooth movement elicits alveolar bone remodeling and orofacial pain that is manifested by tooth mechanical hyperalgesia. Nerve growth factor (NGF) is upregulated in periodontium and may modulate tooth mechanical hyperalgesia. The objectives were to examine the role of NGF in tooth mechanical hyperalgesia and to elucidate the underlying mechanisms. Tooth mechanical hyperalgesia was induced by ligating closed coil springs between incisors and molars in Sprague–Dawley rats. Retrograde labeling was performed by periodontal administration of fluor-conjugated NGF and the detection of fluorescence in trigeminal ganglia (TG). Lentivirus vectors carrying NGF shRNA were employed to knockdown the expression of NGF in TG. The administration of agonists, antagonists, and virus vectors into TG and periodontium was conducted. Tooth mechanical hyperalgesia was examined through the threshold of biting withdrawal. Our results revealed that tooth movement elicited tooth mechanical hyperalgesia that could be alleviated by NGF neutralizing antibody and that NGF was upregulated in periodontium (mainly in periodontal fibroblasts) and TG. Retrograde labeling revealed that periodontal NGF was retrogradely transported to TG after day 1. Acid-sensing ion channel 3 (ASIC3) and NGF were co-expressed in trigeminal neurons and the percentage of co-expression was significantly higher following tooth movement. The administration of NGF and NGF neutralizing antibody into TG could upregulate and downregulate the expression of ASIC3 in TG, respectively. NGF aggravated tooth mechanical hyperalgesia that could be alleviated by ASIC3 antagonist (APETx2). Moreover, NGF neutralizing antibody mitigated tooth mechanical hyperalgesia that could be recapitulated by ASIC3 agonist (GMQ). NGF-based gene therapy abolished tooth mechanical hyperalgesia and downregulated ASIC3 expression. Taken together, in response to force stimuli, periodontal fibroblasts upregulated the expressions of NGF that was retrogradely transported to TG, where NGF elicited tooth mechanical hyperalgesia through upregulating ASIC3. NGF-based gene therapy is a viable method in alleviating tooth-movement-induced mechanical hyperalgesia.

scholarly journals Insulin-like growth factor II binding to the type I somatomedin receptor. Evidence for two high affinity binding sites.

1986 ◽  
Vol 261 (20) ◽  
pp. 9268-9273 ◽  
Author(s):  
S J Casella ◽  
V K Han ◽  
A J D'Ercole ◽  
M E Svoboda ◽  
J J Van Wyk
Keyword(s):  
Growth Factor ◽  
Binding Sites ◽  
Affinity Binding ◽  
Type I ◽  
Insulin Like Growth Factor ◽  
High Affinity Binding ◽  
High Affinity ◽  
Factor Ii

Autoradiographic analysis of 5-HT2A binding sites in the brain of Sardinian alcohol-preferring and nonpreferring rats

1999 ◽  
Vol 373 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Roberto Ciccocioppo ◽  
Stefania Angeletti ◽  
Giancarlo Colombo ◽  
GianLuigi Gessa ◽  
Maurizio Massi
Keyword(s):  
Binding Sites ◽  
Autoradiographic Analysis ◽  
The Brain

scholarly journals Binding Sites and Binding Properties of Binary and Ternary Complexes of Insulin-like Growth Factor-II (IGF-II), IGF-binding Protein-3, and Acid-labile Subunit

1997 ◽  
Vol 272 (44) ◽  
pp. 27936-27942 ◽  
Author(s):  
Ryuji Hashimoto ◽  
Mayumi Ono ◽  
Hiroyuki Fujiwara ◽  
Nobuyuki Higashihashi ◽  
Makoto Yoshida ◽  
...  
Keyword(s):  
Growth Factor ◽  
Binding Sites ◽  
Ternary Complexes ◽  
Binding Properties ◽  
Binary And Ternary Complexes ◽  
Factor Ii ◽  
Igf Binding ◽  
Acid Labile Subunit ◽  
Igf Binding Protein ◽  
Acid Labile

Membrane vesicles of A431 cells contain one class of epidermal growth factor binding sites

1990 ◽  
Vol 1052 (3) ◽  
pp. 453-460 ◽  
Author(s):  
Jos A.M. Berkers ◽  
Paul M.P. van Bergen en Henegouwen ◽  
Arie J. Verkleij ◽  
Johannes Boonstra
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Binding Sites ◽  
Membrane Vesicles ◽  
A431 Cells ◽  
Factor Binding ◽  
Epidermal Growth
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