Integrated Managemenr of Nematodes Parasitic on Prunus SPP

2009 ◽  
pp. 177-194 ◽  
Author(s):  
Soledad VERDEJO-LUCAS ◽  
M. TALAVERA
Keyword(s):  
Prunus Spp

scholarly journals Identification and Sequence Analysis of a Novel Ilarvirus Infecting Sweet Cherry

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 514
Author(s):  
Chrysoula G. Orfanidou ◽  
Fei Xing ◽  
Jun Zhou ◽  
Shifang Li ◽  
Nikolaos I. Katis ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Nucleotide Sequence ◽  
Sanger Sequencing ◽  
Sweet Cherry ◽  
Novel Species ◽  
Peach Tree ◽  
Sweet Cherries ◽  
Blastn Analysis ◽  
Fruit Orchards ◽  
Prunus Spp

In the present study, we utilized high throughput and Sanger sequencing to determine the complete nucleotide sequence of a putative new ilarvirus species infecting sweet cherry, tentatively named prunus virus I (PrVI). The genome of PrVI is comprised of three RNA segments of 3474 nt (RNA1), 2911 nt (RNA2), and 2231 nt (RNA3) and features conserved motifs representative of the genus Ilarvirus. BlastN analysis revealed 68.1–71.9% nt identity of PrVI with strawberry necrotic shock virus (SNSV). In subsequent phylogenetic analysis, PrVI was grouped together with SNSV and blackberry chlorotic ringspot virus (BCRV), both members of subgroup 1 of ilarviruses. In addition, mini-scale surveys in stone fruit orchards revealed the presence of PrVI in a limited number of sweet cherries and in one peach tree. Overall, our data suggest that PrVI is a novel species of the genus Ilarvirus and it consists the fifth member of the genus that is currently known to infect Prunus spp.

scholarly journals Overexpression of a Redox-Regulated Cutinase Gene, MfCUT1, Increases Virulence of the Brown Rot Pathogen Monilinia fructicola on Prunus spp.

2010 ◽  
Vol 23 (2) ◽  
pp. 176-186 ◽  
Author(s):  
Miin-Huey Lee ◽  
Chiu-Min Chiu ◽  
Tatiana Roubtsova ◽  
Chien-Ming Chou ◽  
Richard M. Bostock
Keyword(s):  
Gene Expression ◽  
Redox Regulation ◽  
Brown Rot ◽  
Gus Expression ◽  
Apple Fruit ◽  
Gus Activity ◽  
Monilinia Fructicola ◽  
Gus Reporter ◽  
Flanking Regions ◽  
Prunus Spp

A 4.5-kb genomic DNA containing a Monilinia fructicola cutinase gene, MfCUT1, and its flanking regions were isolated and characterized. Sequence analysis revealed that the genomic MfCUT1 carries a 63-bp intron and a promoter region with several transcription factor binding sites that may confer redox regulation of MfCUT1 expression. Redox regulation is indicated by the effect of antioxidants, shown previously to inhibit MfCUT1 gene expression in cutin-induced cultures, and in the present study, where H2O2 enhanced MfCUT1 gene expression. A β-glucuronidase (GUS) reporter gene (gusA) was fused to MfCUT1 under the control of the MfCUT1 promoter, and this construct was then used to generate an MfCUT1-GUS strain by Agrobacterium spp.-mediated transformation. The appearance of GUS activity in response to cutin and suppression of GUS activity by glucose in cutinase-inducing medium verified that the MfCUT1-GUS fusion protein was expressed correctly under the control of the MfCUT1 promoter. MfCUT1-GUS expression was detected following inoculation of peach and apple fruit, peach flower petals, and onion epidermis, and during brown rot symptom development on nectarine fruit at a relatively late stage of infection (24 h postinoculation). However, semiquantitative reverse-transcriptase polymerase chain reaction provided sensitive detection of MfCUT1 expression within 5 h of inoculation in both almond and peach petals. MfCUT1-GUS transformants expressed MfCUT1 transcripts at twice the level as the wild type and caused more severe symptoms on Prunus flower petals, consistent with MfCUT1 contributing to the virulence of M. fructicola.

scholarly journals Multiyear Evaluation of the Durability of the Resistance Conferred by Ma and RMia Genes to Meloidogyne incognita in Prunus Under Controlled Conditions

2013 ◽  
Vol 103 (8) ◽  
pp. 833-840 ◽  
Author(s):  
Samira Khallouk ◽  
Roger Voisin ◽  
Ulysse Portier ◽  
Joël Polidori ◽  
Cyril Van Ghelder ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Economic Losses ◽  
R Gene ◽  
R Genes ◽  
Tomato Plants ◽  
Controlled Conditions ◽  
Meloidogyne Spp ◽  
Resistance Breakdown ◽  
Susceptible Tomato ◽  
Prunus Spp

Root-knot nematodes (RKNs) (Meloidogyne spp.) are highly polyphagous pests that parasitize Prunus crops in Mediterranean climates. Breeding for RKN-resistant Prunus cultivars, as an alternative to the now-banned use of nematicides, is a real challenge, because the perennial nature of these trees increases the risk of resistance breakdown. The Ma plum resistance (R) gene, with a complete spectrum, and the RMia peach R gene, with a more restricted spectrum, both provide total control of Meloidogyne incognita, the model parthenogenetic species of the genus and the most important RKN in terms of economic losses. We investigated the durability of the resistance to this nematode conferred by these genes, comparing the results obtained with those for the tomato Mi-1 reference gene. In multiyear experiments, we applied a high and continuous nematode inoculum pressure by cultivating nematode-infested susceptible tomato plants with either Prunus accessions carrying Ma or RMia R genes, or with resistant tomato plants carrying the Mi-1 gene. Suitable conditions for Prunus development were achieved by carrying out the studies in a glasshouse, in controlled conditions allowing a short winter leaf fall and dormancy. We first assessed the plum accession ‘P.2175’, which is heterozygous for the Ma gene, in two successive 2-year evaluations, for resistance to two M. incognita isolates. Whatever the isolate used, no nematodes reproducing on P.2175 were detected, whereas galls and nematodes reproducing on tomato plants carrying Mi-1 were observed. In a second experiment with the most aggressive isolate, interspecific full-sib material (P.2175 × [‘Garfi’ almond × ‘Nemared’ peach]), carrying either Ma or RMia (from Nemared) or both (in the heterozygous state) or neither of these genes, was evaluated for 4 years. No virulent nematodes developed on Prunus spp. carrying R genes, whereas galling and virulent individuals were observed on Mi-1-resistant tomato plants. Thus, the resistance to M. incognita conferred by Ma in Prunus material in both a pure-plum and an interspecific genetic background, or by RMia in an interspecific background, appears to be durable, highlighting the value of these two genes for the creation of Prunus rootstock material.

Apiosporina morbosa. [Distribution map].

2002 ◽  
Author(s):  
Keyword(s):  
New York ◽  
North Carolina ◽  
South Carolina ◽  
North America ◽  
New Brunswick ◽  
South Dakota ◽  
Prince Edward Island ◽  
Distribution Map ◽  
Prunus Spp ◽  
New Distribution

Abstract A new distribution map is provided for Apiosporina morbosa (Schwein.) v. Arx Fungi: Ascomycota: Dothideales Hosts: Stone fruit (Prunus spp.). Information is given on the geographical distribution in NORTH AMERICA, Canada, Alberta, British Columbia, Manitoba, New Brunswick, Newfoundland, Northwest, Territories, Nova Scotia, Ontario, Prince Edward Island, Quebec, Saskatchewan, Mexico, USA, Alabama, California, Colorado, Connecticut, Delaware, Florida, Georgia, Idaho, Illinois, Indiana, Iowa, Kentucky, Maine, Maryland, Massachusetts, Michigan, Mississippi, Missouri, Montana, New Jersey, New York, North Carolina, North Dakota, Ohio, Oklahoma, Oregon, Pennsylvania, South Carolina, South Dakota, Tennessee, Texas, Utah, Vermont, Virginia, Washington, West Virginia, Wisconsin.

scholarly journals Eutypa, Eutypella, and Cryptovalsa Species (Diatrypaceae) Associated with Prunus Species in South Africa

Plant Disease ◽  
2018 ◽  
Vol 102 (7) ◽  
pp. 1402-1409 ◽  
Author(s):  
Providence Moyo ◽  
Ulrike Damm ◽  
Lizel Mostert ◽  
Francois Halleen
Keyword(s):  
South Africa ◽  
Phylogenetic Analyses ◽  
Fruit Trees ◽  
Fungal Species ◽  
Stone Fruit ◽  
Prunus Species ◽  
Internal Transcribed Spacer Region ◽  
Eutypa Lata ◽  
Eutypa Dieback ◽  
Prunus Spp

Stone fruit trees (Prunus spp.) are economically important fruit trees cultivated in South Africa. These trees are often grown in close proximity to vineyards and are to a large extent affected by the same trunk disease pathogens as grapevines. The aim of the present study was to determine whether stone fruit trees are inhabited by Diatrypaceae species known from grapevines and whether these trees could act as alternative hosts for these fungal species. Isolations were carried out from symptomatic wood of Prunus species (almond, apricot, cherry, nectarine, peach, and plum) in stone fruit growing areas in South Africa. Identification of isolates was based on phylogenetic analyses of the internal transcribed spacer region and β-tubulin gene. Forty-six Diatrypaceae isolates were obtained from a total of 380 wood samples, from which five species were identified. All five species have also been associated with dieback of grapevine. The highest number of isolates was found on apricot followed by plum. No Diatrypaceae species were isolated from peach and nectarine. Eutypa lata was the dominant species isolated (26 isolates), followed by Cryptovalsa ampelina (7), Eutypa cremea (5), Eutypella citricola (5), and Eutypella microtheca (3). First reports from Prunus spp. are E. cremea, E. citricola, and E. microtheca. Pathogenicity tests conducted on apricot and plum revealed that all these species are pathogenic to these hosts, causing red-brown necrotic lesions like those typical of Eutypa dieback on apricot.

Peach yellows phytoplasma. [Distribution map].

1998 ◽  
Author(s):  
Keyword(s):  
New York ◽  
North Carolina ◽  
South Carolina ◽  
North America ◽  
Geographical Distribution ◽  
Prunus Persica ◽  
Distribution Map ◽  
Prunus Spp ◽  
New Distribution

Abstract A new distribution map is provided for Peach yellows phytoplasma Bacteria: Phytoplasmas Hosts: Peach (Prunus persica), also other Prunus spp. Information is given on the geographical distribution in NORTH AMERICA, Canada, Ontario, USA, Illinois, Indiana, Kentucky, Maryland, Michigan, New York, North Carolina, Ohio, Pennsylvania, South Carolina, Tennessee, Texas, Virginia.

Peach rosette phytoplasma. [Distribution map].

1998 ◽  
Author(s):  
Keyword(s):  
North America ◽  
Geographical Distribution ◽  
Prunus Persica ◽  
Distribution Map ◽  
Prunus Spp ◽  
New Distribution

Abstract A new distribution map is provided for Peach rosette phytoplasma Bacteria: Phytoplasmas Hosts: Peach (Prunus persica), also P. salicina and other Prunus spp. Information is given on the geographical distribution in NORTH AMERICA, USA, Arkansas, Georgia, Texas.

Fomes fomentarius. [Distribution map].

2002 ◽  
Author(s):  
Keyword(s):  
New York ◽  
North Carolina ◽  
Distribution Map ◽  
Jammu And Kashmir ◽  
Fomes Fomentarius ◽  
Central Russia ◽  
Southern Russia ◽  
Prunus Spp ◽  
New Distribution ◽  
Distribution In Europe

Abstract A new distribution map is provided for Fomes fomentarius (L.) Kickx Fungi: Basidiomycota: Polyporales Hosts: Various broad-leaved trees including beech (Fagus sylvatica), birch (Betula spp.), cherry (Prunus spp.), poplar (Populus spp.) and oak (Quercus spp.). Information is given on the geographical distribution in EUROPE, Austria, Belarus, Bulgaria, Denmark, Finland, France, Germany, Italy, Luxembourg, Norway, Portugal, Romania, Central Russia Russia, Southern Russia, Western Siberia, Slovakia, Sweden, UK, Ukraine, Yugoslavia (Fed. Rep.) ASIA, China, India, Jammu and Kashmir, Iran, Japan, Pakistan, Uzbekistan, AFRICA, Algeria, NORTH AMERICA, Canada, Alberta, British Columbia, Nova Scotia, USA, Alabama, Alaska, California, Idaho, Maine, Maryland, Michigan, Minnesota, Montana, New Hampshire, New Jersey, New York, North Carolina, North Dakota, Ohio, Oregon, Virginia, Washington, West Virginia, Wisconsin.

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