Studies on Antimicrobial Activity and Certain Chemical Parameters of Freeze-Dried Wild Plums (Prunus Spp.)

2009 ◽  
Vol 8 (9) ◽  
pp. 1434-1441 ◽  
Author(s):  
Seyhun Yurdugul ◽  
Faruk Bozoglu
2012 ◽  
Vol 47 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Nasima Akhtar ◽  
Monzur Morshed Ahmeda ◽  
Nishat Sarker ◽  
Khandaker Rayhan Mahbuba ◽  
Abdul Matin Sarker

Growth response of Spirulina platensis in papaya skin extract media and their antimicrobial activity were studied. Five different concentrations  e.g. 10gm/L, 8gm/L, 6 gm/L, 4 gm/L and 2gm/L of Papaya (Carica papaya) skin extract media and BD1 (control) medium were used  in this study. After 8 days of cultivation, the optical density (0.33) was recorded in BD1 medium and among the five different concentrations  of papaya skin extract media the maximum was found (0.31) in 6gm/L. Antimicrobial activity of Spirulina platensis grown in three  media namely Zarrouk, BD1 media and media made from papaya skin extract was also studied. Only freeze dried Spirulina platensis powder  extract showed inhibitory effect against bacteria and no antifungal activity was observed. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11445 Bangladesh J. Sci. Ind. Res. 47(2), 147-152, 2012  


2013 ◽  
Vol 4 (3) ◽  
pp. 176-183 ◽  
Author(s):  
Daiana Jaqueline Gatti Neres ◽  
Sidnei Emilio Bordignon Junior ◽  
César Milton Baratto

The searches for new antimicrobial compounds to control the lactic acid bacteria in winemaking are based in the increasing global concern about the chemical preservatives restrictions and the development of super-resistant strains continuously exposed to sulfites in wineries for decades. The antimicrobial activity of the nisin as a biopreservative was studied for Bordô (Ives) and Niágara grape wines from Santa Catarina, Brazil. Measurements of antimicrobial activity were made by well-diffusion assays. From the eleven previously assessed strains of nisinsusceptible lactic bacteria (nisin concentration 1000 IU ml-1), four were selected for definitive assays with nisin (100 IU ml-1) dissolved in the wines. Positive results for inhibition were obtained for the four strains selected. Next, the direct inhibitory action was assessed in wines artificially inoculated and then treated with nisin. After 60 days of storage, there was reduction in the total bacterial population as compared to control, especially in Bordô (Ives) wine, while the physic-chemical parameters were not influenced by the nisin treatment. The inhibitory activity of nisin was not affected when it was dissolved in wine in the antimicrobial assays, and its potential utilization as biopreservative should be able to aid on the control of autochthonous microbiota, but further studies are required to conclude more precisely the nisin effects at long term in wines.


F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1200
Author(s):  
Cecilia Barba Guevara ◽  
Luis Montaluisa ◽  
María Elena Maldonado Rodriguez

Background: This research was carried out in order to study the antimicrobial effectiveness of crude latex of two varieties of "Sangre de Drago": Croton lechleri Muller Arg. and Croton urucurana Baill and compare that effectiveness to the antimicrobial activity of the alcoholic extracts of its leaves and bark.  Methods: The activity of the alcoholic extracts and latex were evaluated against bacterial strains of Staphylococcus epidermidis, Bacillus subtilis and Escherichia coli. The extraction of the alcoholic extracts (20% Tincture) of the leaves, bark and latex from the two Croton species was carried out by maceration using 70% alcohol as a menstruum, at room temperature, for 2 to 7 days, with shaking at least twice a day.  A 20% tincture was obtained, from which the physical and chemical parameters were determined as indicated by the Ecuadorian Quality Control Standard for natural medicinal products. Results: It was found that both the alcoholic extracts of the plant material and the crude latex indicate antimicrobial activity for S. epidermidis, moderate antimicrobial activity for B. subtilis and no antimicrobial activity for E. coli. The moderate antimicrobial activity against B. subtilis, at doses of 125 p.p.m., is in line with the findings of previous studies by other authors.  Conclusions: he antimicrobial activity of the latex of the two species against S. epidermidis is not registered in literature and, the negative antimicrobial activity for E. coli does not agree with what has been reported by previous studies.


2014 ◽  
pp. 99-116 ◽  
Author(s):  
Jelena Vulic ◽  
Aleksandra Velicanski ◽  
Dragana Cetojevic-Simin ◽  
Vesna Tumbas-Saponjac ◽  
Sonja Djilas ◽  
...  

The main chemical composition, i.e. the total content of bioactive compounds (phenolics 2209.86 ? 70.32 mg GAE/100g FDR, flavonoids 831.87 ? 12.61 mg R/100g FDR and anthocyanins 144.55 ? 0.39 mg CGE/100g FDR), in freeze-dried raspberry (FDR) was evaluated spectrophotometrically. Vitamin C content was determined by HPLC analysis (88.81 ? 4.38 mg vit C/100g FDR). Antioxidant activities of FDR extract were evaluated spectrophotometrically on stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and by electron spin resonance spectroscopy (ESR) method on hydroxyl radicals (?OH). EC50 values were evaluated. EC50 DPPH? was 0.127 ? 0.013 mg/ml, while EC50 ?OH was 1.366 ? 0.026 mg/ml. Antiproliferative activity of the FDR extract was evaluated in vitro in three human cell lines by colorimetric sulphorhodamine B (SRB) assay. The most pronounced effects were obtained in the breast adenocarcinoma cell line (MCF7). EC50 value was 395.07 ? 96.38 ?g/ml. Antimicrobial activity was determined by disk diffusion method. The FDR extract produced a clear inhibition zone (without visible colonies) only toward Staphylococcus aureus. The minimal inhibitory (MIC) and minimal bactericidal (MBC) concentrations of FDR extract were evaluated. The values MIC were in the range of 4.7 - 100 mg/ml, and of MBC in the range of 6.3 - > 100 mg/ml.


2021 ◽  
pp. 94-101
Author(s):  
Vaishali Gupta ◽  
Deepak Vyas

Different types of peptides are produced by cyanobacteria of the genus Nostoc, which are unique in structure and have a wide spectrum of biological activities. The objective of the study to explore different habitats of organism and study antimicrobial activities to improve their pharmaceutical application and drug like properties by structure modification. A cyclic peptide nostophycin was isolated from Nostoc calcicola (MK506349) through freeze dried lyophilization method. Its structure has been elucidated with FT-IR, 1HNMR, 13CNMR and LC-MS. Glycine, d-glutamine, l-phenylamine, d-isoleucine, l-proline and a novel amino acid Ahoa are constituents of nostophycin. 1HNMR, 13CNMR spectroscopy confirmed the number of protons and carbons, and characteristics peak determined the structure and fragmentation pattern through LCMS.  Nostophycin possess Ahoa instead of Adha which makes it different from microcystin. Nostophycin exhibits antimicrobial activity against E.coli, S. aureus, C. albicens and A. niger. A good antifungal activity (9-52 μg/mL) and moderate  antimicrobial activity (concentration 18-52 μg/mL) were found for nostophycin. In case of already known peptides, these molecules may be further exploited to improve pharmaceutical application and future drug development.


2020 ◽  
Vol 9 (3) ◽  
pp. e119932580
Author(s):  
Edilayane da Nóbrega Santos ◽  
Erick dos Anjos Bezerra ◽  
Jeniffer Viviany dos Santos Fonseca ◽  
Mychelle de Lira Andrade ◽  
João Vitor Fonseca Feitoza ◽  
...  

The bioactive properties of unconventional fruits have been of interest in recent research. The objective of this study was to evaluate the bioactivity of Terminalia catappa Linn fruit and seed through its antioxidant and antimicrobial activity against pathogenic microorganisms. The fruit and the seed were analyzed for the physical-chemical parameters of pH, aw, humidity, ashes, carbohydrates, proteins and lipids. After drying, pulp and almond flours were evaluated for bioactivity (total phenolics, tannins, anthocyanins and flavonoids and antioxidant-DPPH) and antimicrobial activity. The fruit had a higher carbohydrate content (15.65%), while the seed was characterized by high lipid content (48.52% ± 0.31) and protein (26.40% ± 0.10). The pulp flour showed significant amounts of tannins (1662.68 mg.100-1 g ± 1.27) and antimicrobial activity inhibiting Staphylococcus aureus, Pseudomonas putida, Pseudomonas aeruginosa, Clostridium perfringens and Listeria monocytogenes. The seed flour presented antioxidant activity (46.05 mg.100-1 g ± 0.23), but did not present inhibitory power against the tested bacterial strains. Terminalia catappa Linn fruit can be an alternative new ingredient with natural antioxidant action.


Author(s):  
Lamia Singer ◽  
Gabriele Bierbaum ◽  
Katja Kehl ◽  
Christoph Bourauel

AbstractLiterature lacks sufficient data regarding addition of natural antibacterial agents to glass ionomer cement (GICs). Hence, the aim of the study was to increase the antimicrobial properties of GICs through its modification with mixture of plant extracts to be evaluated along with an 0.5% chlorohexidine-modified GIC (CHX-GIC) with regard to biological and compressive strength properties. Conventional GIC (freeze-dried version) and CHX were used. Alcoholic extract of Salvadora persica, Olea europaea, and Ficus carcia leaves were prepared using a Soxhlet extractor for 12 h. The plant extract mixture (PE) was added in three different proportions to the water used for preparation of the dental cement (Group 1:1 PE, 2:1 PE, and 1:2 PE). Specimens were then prepared and tested against the unmodified GIC (control) and the 0.5% CHX-GIC. Chemical analysis of the extract mixture was performed using Gas chromatography–mass spectrometry. Antimicrobial activity was evaluated using agar diffusion assay against Micrococcus luteus and Streptoccocus mutans. Compressive strength was evaluated according to ISO 9917-1:2007 using a Zwick testing machine at a crosshead speed of 0.5 mm/min. Antimicrobial activity against Streptoccocus mutans was significantly increased for all the extract-modified materials compared to the unmodified cement, and the highest concentration was comparable to the CHX-GIC mixture. The activity against Micrococcus luteus was also significantly increased, but only for the material with the highest extract concentration, and here the CHX-GIC group showed statistically the highest antimicrobial activity. Compressive strength results revealed that there was no statistically significant difference between the different mixtures and the control except for the highest tested concentration that showed the highest mean values. The plant extracts (PEs) enhanced the antimicrobial activity against S. mutans and also against M. luteus in the higher concentration while compressive strength was improved by addition of the PE at higher concentrations.


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