Novel classes of fatty acid and retinol binding protein from nematodes

1999 ◽  
pp. 69-75
Author(s):  
Lindsay McDermott ◽  
Alan Cooper ◽  
Malcolm W. Kennedy
Keyword(s):  
Fatty Acid ◽  
Binding Protein ◽  
Retinol Binding Protein

scholarly journals Cellular retinol-binding protein type III is a PPARγ target gene and plays a role in lipid metabolism

2008 ◽  
Vol 295 (6) ◽  
pp. E1358-E1368 ◽  
Author(s):  
Cynthia F. Zizola ◽  
Gary J. Schwartz ◽  
Silke Vogel
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Lipid Metabolism ◽  
Free Fatty Acid ◽  
Target Gene ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Wild Type ◽  
Type Iii ◽  
Cellular Retinol Binding Protein

Cellular retinol-binding protein (CRBP) type III (CRBP-III) belongs to the family of intracellular lipid-binding proteins, which includes the adipocyte-binding protein aP2. In the cytosol, CRBP-III binds retinol, the precursor of retinyl ester and the active metabolite retinoic acid. The goal of the present work is to understand the regulation of CRBP-III expression and its role in lipid metabolism. Using EMSAs, luciferase reporter assays, and chromatin immunoprecipitation assays, we found that CRBP-III is a direct target of peroxisome proliferator-activated receptor-γ (PPARγ). Moreover, CRBP-III expression was induced in adipose tissue of mice after treatment with the PPARγ agonist rosiglitazone. To examine a potential role of CRBP-III in regulating lipid metabolism in vivo, CRBP-III-deficient (C-III-KO) mice were maintained on a high-fat diet (HFD). Hepatic steatosis was decreased in HFD-fed C-III-KO compared with HFD-fed wild-type mice. These differences were partly explained by decreased serum free fatty acid levels and decreased free fatty acid efflux from adipose tissue of C-III-KO mice. In addition, the lack of CRBP-III was associated with reduced food intake, increased respiratory energy ratio, and altered body composition, with decreased adiposity and increased lean body mass. Furthermore, expression of genes involved in mitochondrial fatty acid oxidation in brown adipose tissue was increased in C-III-KO mice, and C-III-KO mice were more cold tolerant than wild-type mice fed an HFD. In summary, we demonstrate that CRBP-III is a PPARγ target gene and plays a role in lipid and whole body energy metabolism.

scholarly journals Diet-related variation in cellular retinol-binding protein type II gene expression in rat jejunum

2005 ◽  
Vol 94 (6) ◽  
pp. 890-895 ◽  
Author(s):  
Kazuhito Suruga ◽  
Masaaki Kitagawa ◽  
Hiromitsu Yasutake ◽  
Sachiko Takase ◽  
Toshinao Goda
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Binding Protein ◽  
Dietary Fatty Acids ◽  
Retinol Binding Protein ◽  
Rat Jejunum ◽  
Type Ii ◽  
Feeding Period ◽  
Related Variation ◽  
Cellular Retinol Binding Protein

Cellular retinol-binding protein type II (CRBPII) is involved in the transport of vitamin A and its metabolism in the small intestine. In the present study, we demonstrated diet-related variations in CRBPII expression in rat jejunum. The CRBPII protein and mRNA levels increased in parallel after the start of feeding period regardless of whether the feeding period was restricted to the hours of darkness or of light. In addition, this variation was observed in the rats fed high-fat diet or low-fat diets, but not in those fed a fat-free diet or in fasted rats. A similar diet-induced variation was seen in the mRNA of liver-type fatty acid-binding protein in rat jejunum. In the transient transfection experiment, unsaturated fatty acid increased rat CRBPII gene promoter activity via the PPARα/retinoid X receptor-α heterodimer. Taken together, these results suggest that the diet-related variation in CRBPII expression in rat jejunum may be brought about by the transcriptional induction of CRBPII gene expression mainly triggered by dietary fatty acids.

scholarly journals Fatty Acid and Retinol-Binding Protein: Unusual Protein Conformational and Cavity Changes Dictated by Ligand Fluctuations

2019 ◽  
Vol 59 (8) ◽  
pp. 3545-3555
Author(s):  
G. P. Barletta ◽  
G. Franchini ◽  
B. Corsico ◽  
S. Fernandez-Alberti
Keyword(s):  
Fatty Acid ◽  
Binding Protein ◽  
Retinol Binding Protein

scholarly journals Fatty Acid-and Retinol-Binding Protein, Mj-FAR-1 Induces Tomato Host Susceptibility to Root-Knot Nematodes

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e64586 ◽  
Author(s):  
Ionit Iberkleid ◽  
Paulo Vieira ◽  
Janice de Almeida Engler ◽  
Kalia Firester ◽  
Yitzhak Spiegel ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Binding Protein ◽  
Host Susceptibility ◽  
Retinol Binding Protein ◽  
Root Knot Nematodes

scholarly journals 1H, 13C and 15N chemical shift assignments of Na-FAR-1, a helix-rich fatty acid and retinol binding protein of the parasitic nematode Necator americanus

2012 ◽  
Vol 8 (1) ◽  
pp. 19-21 ◽  
Author(s):  
M. Florencia Rey-Burusco ◽  
Marina Ibañez-Shimabukuro ◽  
Alan Cooper ◽  
Malcolm W. Kennedy ◽  
Betina Córsico ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chemical Shift ◽  
Parasitic Nematode ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Chemical Shift Assignments ◽  
Necator Americanus
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