ODC-Mediated Biosynthesis and DAO-Mediated Catabolism of Putrescine Involved in the Induction of Rooting by Leaf Explants of Chrysanthemum morifolium Ramat In Vitro

An efficient and rapid in vitro regeneration protocol was developed for chrysanthemum (Chrysanthemum morifolium Ramat) using two local varieties of Bangladesh namely, BARI Chrysanthemum-2 (BARI Chry-2) and local yellow (Y). MS medium supplemented with nine different concentrations and combinations of BAP and IAA was employed to optimize regeneration protocol using young in vitro derived leaf explants. Direct organogenesis was observed from the leaf explants on MS medium supplemented with 0.5 mg/l BAP and 2.0 mg/l IAA (T6) for both the varieties. This treatment (T6) induced shoot buds directly on the adaxial surface of the leaf providing the highest regeneration percentage (90% for BARI Chry-2 and 94.73% for Y), the highest number of shoot/explant (7.6 for BARI Chry-2 and 8.6 for Y) and maximum length of the shoot after six weeks (3 cm for BARI Chry-2 and 2.9 cm for Y) of culture. Explants with initially regenerated shoots were subculture on hormone free MS medium for shoot elongation after 4 weeks of their inoculation. During elongation of shoots, 90-95% of the regenerated shoots produced roots spontaneously in hormone free MS medium within 7-8 weeks of their inoculation. Rooted plantlets were transplanted to the field following hardening where 100% plantlets were survived and produced flower without any variation. Plant Tissue Cult. & Biotech. 31(2): 161-171, 2021 (December)

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Callus Induction of Leaves and Stems in Krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) with Alternative Foliar Fertilizers Media

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2020.92.109-115 ◽

2021 ◽

Vol 9 (2) ◽

pp. 109-115

Author(s):

Ahmad Saifun Naser ◽

Muhammad Wisnu

Keyword(s):

Tissue Culture ◽

Callus Formation ◽

Leaf Explants ◽

Chrysanthemum Morifolium ◽

Stem Explants ◽

Appearance Time ◽

Randomized Design ◽

The Media ◽

Completely Randomized Design ◽

Chrysanthemum Morifolium Ramat

Availability of quality seeds in production of krisan (Chrysanthemum morifolium Ramat cv Dewi ratih) cultivation is still rare, therefore research on seed multiplication through tissue culture is needed. The media used in tissue culture is relatively expensive for home industry. This study aims to determine the respond of leaf and stem explants using foliar fertilizers (Growmore, Gandasil D and Mutiara) as an alternative media for callus inductions. This study used a Completely Randomized Design (CRD) consisted of 4 treatments: P0: ½ MS + 0,25 mg/l BAP, P1 (Growmore + 0,25 mg/l BAP), P2 (Gandasil D + 0,25 mg/l BAP), P3 (Mutiara + 0,25 mg/l BAP). The variables observed in this study included callus appearance time, callus color and callus texture. The result of this study indicated that the use of BAP (6-Benzyl Amino Purine) affected the time of callus formation and callus morphology. Callus was formed on leaf explants 13 days after planting while on stem explants 7 days after planting and compact texture. Growmore + 0,25 mg/l BAP treatment yields the best callus on leaf explant, while Gandasil D + 0,25 mg/l BAP treatment yields the best callus on stem explant.

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NANO BẠC TRONG KHỬ TRÙNG MÔI TRƯỜNG NUÔI CẤY IN VITRO CÂY HOA CÚC (CHRYSANTHEMUM MORIFOLIUM RAMAT CV. JIMBA)

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/15/3/13385 ◽

2018 ◽

Vol 15 (3) ◽

pp. 505-513

Author(s):

Dương Tấn Nhựt ◽

Hoàng Thanh Tùng ◽

Lương Thiện Nghĩa ◽

Nguyễn Duy Anh ◽

Nguyễn Phúc Huy ◽

...

Keyword(s):

Chrysanthemum Morifolium ◽

Chrysanthemum Morifolium Ramat

Phương pháp nhân giống in vitro được chứng minh là phương pháp hữu hiệu để nhân giống cây trồng với số lượng lớn trong thời gian ngắn và trở thành một công cụ hữu hiệu cho công tác chọn, tạo giống cây trồng. Tuy nhiên, phương pháp này vẫn còn những tồn tại mà nổi bật lên là vấn đề nhiễm vi sinh vật trong quá trình nuôi cấy, chúng làm giảm chất lượng, tăng khả năng mất nguồn giống. Khử trùng môi trường nuôi cấy là vấn đề bắt buộc đối với quá trình vi nhân giống, đây là giai đoạn tiêu tốn nhiều điện năng và trải qua nhiều công đoạn. Bên cạnh đó, việc hấp khử trùng môi trường trong một thời gian làm giảm hoạt tính của các chất điều hòa sinh trưởng thực vật cũng như thành phần dinh dưỡng của môi trường. Nghiên cứu này hướng đến việc ứng dụng nano bạc như một biện pháp thay thế cho phương pháp khử trùng môi trường truyền thống. Trong nghiên cứu này, chúng tôi bổ sung các nồng độ khác nhau của nano bạc (0 - 5 ppm), đường (0 - 30 g/l) và than hoạt tính (0 - 1g/l) vào môi trường nuôi cấy in vitro cây hoa cúc và không hấp khử trùng môi trường nhằm đánh giá khả năng tiệt trùng môi trường cũng như cảm ứng sự sinh trưởng và phát triển của cây. Kết quả ghi nhận được cho thấy, bổ sung 4 ppm nano bạc, không bổ sung than hoạt tính và nồng độ đường từ 0 - 20 g/l vào môi trường nuôi cấy không cấy mẫu cho hiệu quả khử trùng 100% sau 4 tuần. Cây cúc cho sự sinh trưởng và phát triển tốt trong môi trường bổ sung 4 ppm nano bạc, 20 g/l đường, 5 g/l agar và không hấp khử trùng.

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In vitro regeneration of two chrysanthemum (Chrysanthemum morifolium Ramat.) cultivars through organogenesis from petal explants

International Journal of Biosciences (IJB) ◽

10.12692/ijb/5.4.76-81 ◽

2014 ◽

Vol 5 (4) ◽

pp. 76-81

Keyword(s):

In Vitro Regeneration ◽

Chrysanthemum Morifolium ◽

Chrysanthemum Morifolium Ramat

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Characterization of Composition and Antifungal Properties of Leaf Secondary Metabolites from Thirteen Cultivars of Chrysanthemum morifolium Ramat

Molecules ◽

10.3390/molecules24234202 ◽

2019 ◽

Vol 24 (23) ◽

pp. 4202 ◽

Cited By ~ 2

Author(s):

Huanhuan Xue ◽

Yifan Jiang ◽

Hongwei Zhao ◽

Tobias G. Köllner ◽

Sumei Chen ◽

...

Keyword(s):

Secondary Metabolites ◽

Antifungal Agents ◽

Pathogenic Fungi ◽

Chrysanthemum Morifolium ◽

In Vitro Assays ◽

Gas Chromatography Mass Spectrometry ◽

Leaf Extracts ◽

Antifungal Properties ◽

Chrysanthemum Morifolium Ramat

Chrysanthemum morifolium Ramat is an ornamental plant of worldwide cultivation. Like many other species in the family Asteraceae, C. morifolium is a rich producer of secondary metabolites. There are two objectives in this study: (I) to determine and compare the diversity of apolar secondary metabolites among different cultivars of C. morifolium and (II) to compare their properties as antifungal agents. To attain these objectives, we selected 13 cultivars of C. morifolium that are commonly used for making chrysanthemum tea as experimental materials. Leaves at the same developmental stage were collected from respective mature plants and subjected to organic extraction. The extracts were analyzed using gas chromatography–mass spectrometry. A total of 37 apolar secondary metabolites including 26 terpenoids were detected from the 13 cultivars. These 13 cultivars can be largely divided into three chemotypes based on chemical principal components analysis. Next, the extracts from the 13 cultivars were examined in in vitro assays for their antifungal properties against three species of pathogenic fungi: Fusarium oxysporum, Magnaporthe oryzae, and Verticillium dahliae. Significant variability in antifungal activity of the leaf extracts among different cultivars was observed. The 13 cultivars can be divided into four groups based on their antifungal activities, which could be partly correlated to the contents of terpenoids. In short, this study reveals large variations in chemical composition, particularly of terpenoids, of leaf secondary metabolites among different cultivars of C. morifolium and their different abilities in functioning as antifungal agents.

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Calcium, potassium and magnesium treatment of Chrysanthemum morifolium cv. "Bi Time" and callogenesis in vitro

Scientia Agricola ◽

10.1590/s0103-90162002000400011 ◽

2002 ◽

Vol 59 (4) ◽

pp. 689-693 ◽

Cited By ~ 4

Author(s):

Fábio Borgatto ◽

Carlos Tadeu dos Santos Dias ◽

Antônio Francisco Campos Amaral ◽

Murilo Melo

Keyword(s):

Calcium Concentration ◽

Solid Medium ◽

Leaf Explants ◽

Chrysanthemum Morifolium ◽

Magnesium Concentration ◽

Donor Plant ◽

Calcium Effect ◽

Magnesium Treatment ◽

Different Levels

The chemical composition and vegetative vigor of the donor plant are essential for the satisfactory performance of explants in vitro. In order to test the effect of potassium, calcium and magnesium nutritional status of Chrysanthemum morifolium plants on callogenesis in vitro, pot plants growing in sand were irrigated with nutrient solution containing different levels of potassium (0; 58.5; 117 and 234 mg L-1), calcium (0; 50; 100 e 200 mg L-1) and magnesium (0;12; 48 e 96 mg L-1). After 30 and 45 days, explants (shoot segments and leaf discs) were collected, desinfected and inoculated on MS solid medium supplemented with 0.1 mg L-1 of kinetin and 5.0 mg L-1 of nafthalene acetic acid for callogenesis induction. Callogenesis evaluated as callus fresh weight was affected by nutrients treatment. Callus growth on leaf explants was inversely proporcional to potassium concentration and directly proportional to magnesium concentration in shoot explants. The calcium effect on callogensis of leaf explants was dependent on treatment duration. For 30 days treatment callogenseis was inversely related to calcium concentration and after 45 days was directly related to calcium concentration.

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