Two-step test system using the plant-dwelling non-target insect Coccinella septempunctata to generate data for registration of pesticides

Ecotoxicology ◽  
1998 ◽  
pp. 114-122 ◽  
Author(s):  
Richard Schmuck ◽  
M. Candolfi ◽  
R. Kleiner ◽  
P. Klepka ◽  
S. Nengel ◽  
...  
Keyword(s):  
Coccinella Septempunctata ◽  
Test System ◽  
Step Test

Three-Step Test System for the Identification of Novel GABAA Receptor Modulating Food Plants

2016 ◽  
Vol 71 (4) ◽  
pp. 355-360 ◽  
Author(s):  
Sümeyye Sahin ◽  
Volker Eulenburg ◽  
Wolfgang Kreis ◽  
Carmen Villmann ◽  
Monika Pischetsrieder
Keyword(s):  
Gabaa Receptor ◽  
Test System ◽  
Food Plants ◽  
Step Test

Immunogold labeling of CMP-KDO synthetase produced by recombinant E. Coli cells

1987 ◽  
Vol 45 ◽  
pp. 924-925
Author(s):  
F. A. Durum ◽  
R. G. Goldman ◽  
T. J. Bolling ◽  
M. F. Miller
Keyword(s):  
Inclusion Bodies ◽  
Large Scale ◽  
Recombinant Dna ◽  
Test System ◽  
Cell Protein ◽  
Gram Negative Bacteria ◽  
Cytoplasmic Inclusions ◽  
Isolation And Purification ◽  
E Coli ◽  
Low Concentrations

CMP-KDO synthetase (CKS) is an enzyme which plays a key role in the synthesis of LPS, an outer membrane component unique to gram negative bacteria. CKS activates KDO to CMP-KDO for incorporation into LPS. The enzyme is normally present in low concentrations (0.02% of total cell protein) which makes it difficult to perform large scale isolation and purification. Recently, the gene for CKS from E. coli was cloned and various recombinant DNA constructs overproducing CKS several thousandfold (unpublished data) were derived. Interestingly, no cytoplasmic inclusions of overproduced CKS were observed by EM (Fig. 1) which is in contrast to other reports of large proteinaceous inclusion bodies in various overproducing recombinant strains. The present immunocytochemical study was undertaken to localize CKS in these cells.Immune labeling conditions were first optimized using a previously described cell-free test system. Briefly, this involves soaking small blocks of polymerized bovine serum albumin in purified CKS antigen and subjecting them to various fixation, embedding and immunochemical conditions.

Real Ear Sound Pressure Levels Developed by Three Portable Stereo System Earphones

1992 ◽  
Vol 1 (4) ◽  
pp. 52-55 ◽  
Author(s):  
Gail L. MacLean ◽  
Andrew Stuart ◽  
Robert Stenstrom
Keyword(s):  
High Frequency ◽  
Sound Pressure ◽  
Low Frequency ◽  
Test System ◽  
Output Frequency ◽  
Frequency Effects ◽  
Adult Men ◽  
Frequency Responses ◽  
Significant Difference ◽  
Sound Pressure Levels

Differences in real ear sound pressure levels (SPLs) with three portable stereo system (PSS) earphones (supraaural [Sony Model MDR-44], semiaural [Sony Model MDR-A15L], and insert [Sony Model MDR-E225]) were investigated. Twelve adult men served as subjects. Frequency response, high frequency average (HFA) output, peak output, peak output frequency, and overall RMS output for each PSS earphone were obtained with a probe tube microphone system (Fonix 6500 Hearing Aid Test System). Results indicated a significant difference in mean RMS outputs with nonsignificant differences in mean HFA outputs, peak outputs, and peak output frequencies among PSS earphones. Differences in mean overall RMS outputs were attributed to differences in low-frequency effects that were observed among the frequency responses of the three PSS earphones. It is suggested that one cannot assume equivalent real ear SPLs, with equivalent inputs, among different styles of PSS earphones.

Pre-clinical evaluation of a virtual reality neuropsychological test system: Occurence of side effects

2000 ◽  
Author(s):  
Tibor I. Kesztyues ◽  
M. Mehlitz ◽  
E. Schilken ◽  
G. Weniger ◽  
S. Wolf ◽  
...  
Keyword(s):  
Virtual Reality ◽  
Side Effects ◽  
Clinical Evaluation ◽  
Neuropsychological Test ◽  
Test System

The Diagnosis of Lupus Anticoagulants by the Activated Partial Thromboplastin Time - The Central Role of Phosphatidyl Serine

1984 ◽  
Vol 52 (02) ◽  
pp. 172-175 ◽  
Author(s):  
P R Kelsey ◽  
K J Stevenson ◽  
L Poller
Keyword(s):  
Screening Method ◽  
Coagulation Factors ◽  
Phosphatidyl Serine ◽  
Test System ◽  
Specific Factor ◽  
Marginal Effect ◽  
Constant Composition ◽  
Lupus Anticoagulants ◽  
Different Types

SummaryLiposomes of pure phospholipids were used in a modified APTT test system and the role of phosphatidyl serine (PS) in determining the sensitivity of the test system to the presence of lupus anticoagulants was assessed. Six consecutive patients with lupus anticoagulants and seven haemophiliacs with anticoagulants directed at specific coagulation factors, were studied. Increasing the concentration of phospholipid in the test system markedly reduced the sensitivity to lupus anticoagulants but had marginal effect on the specific factor inhibitors. The same effect was achieved when the content of PS alone was increased in a vehicle liposome of constant composition.The results suggest that the lupus anticoagulants can best be detected by a screening method using an APTT test with a reagent of low PS content. The use of a reagent rich in PS will largely abolish the lupus anticoagulant’s effect on the APTT. An approach using the two different types of reagent may facilitate differentiation of lupus inhibitors from other types of anticoagulant.

Strategi Penyelenggaraan Diklat Dan Ujian Sertifikasi Keahlian Pengadaan Barang/ Jasa Pemerintah Di Lingkungan Pemerintah Kabupaten Magelang

2017 ◽  
Vol 24 (1) ◽  
pp. 87-106
Author(s):  
Wiharyanto Wiharyanto
Keyword(s):  
Education And Training ◽  
Test System ◽  
Civil Servants ◽  
Quality Of Education ◽  
Alternative Formulation ◽  
Computer Assisted ◽  
Certification Exams ◽  
The Government ◽  
And Training

The study aims to analyze about the low graduation and certification exam training participants of the procurement of goods / services of the government and its contributing factors, and formulate a strategy of education and training and skills certification exams procurement of goods / services of the government. Collecting data using the method of study documentation, interviews, and questionnaires. Is the official source of information on the structural and functional Regional Employment Board, as well as the participants of the training and skills certification exams procurement of goods / services of the government in Magelang regency government environment. Analysis using 4 quadrant SWOT analysis, to determine the issue or strategic factors in improving the quality of education and training and skills certification exams procurement of government goods / services within the Government of Magelang regency. The results show organizer position is in quadrant I, which is supporting the growth strategy, with 3 alternative formulation strategies that improve the quality of education and training and skills certification exams procurement of government goods / services, and conducts certification examination of the procurement of government goods / services with computer assisted test system (CAT). Based on the research recommendations formulated advice to the organizing committee, namely: of prospective participants of the training and skills certification exams procurement of goods / services the government should consider the motivation of civil servants, is examinees who have attended training in the same period of the year, the need for simulation procurement of goods / services significantly, an additional allocation of training time, giving sanction to civil servants who have not passed the exam, the provision of adequate classroom space with the number of participants of each class are proportional, as well as explore the evaluation of education and training and skills certification exams procurement of goods / services for Government of participants.

STUDIES ON A NON-MELATONIN PINEAL ANTI-GONADOTROPHIN

1972 ◽  
Vol 69 (2) ◽  
pp. 257-266 ◽  
Author(s):  
Bryant Benson ◽  
Mary Jane Matthews ◽  
Alvin E. Rodin
Keyword(s):  
Solvent Extraction ◽  
Organic Solvent ◽  
Pineal Gland ◽  
Gel Filtration ◽  
Test System ◽  
Pineal Body ◽  
Physiological Effects ◽  
Organic Solvent Extraction ◽  
Gland Function

ABSTRACT Continuing investigation of pineal gland function indicates that the anti-gonadotrophic activity of this organ cannot be attributed solely to the postulated hormone melatonin, the concentration of which is negligible in the pineal body compared to quantities required to produce unequivocal physiological effects. A non-melatonin antigonadotrophic substance recently isolated from bovine pineal glands was further purified by organic solvent extraction, ultrafiltration and gel filtration. Studies of partial blockage of compensatory ovarian hypertrophy in unilaterally ovariectomized Charles River CD-1 mice indicated that this substance is significantly more potent than melatonin in this test system.

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