The Tissue Culture Study of Antifungal Agents and Their Morphological Changes on Yeast and Yeast-Like Fungi

1976 ◽  
pp. 157-163
Author(s):  
A. Uetsuka ◽  
S. Satoh ◽  
M. Itoh ◽  
N. Okazaki ◽  
Y. Ohno ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Antifungal Agents ◽  
Morphological Changes ◽  
Culture Study

scholarly journals Feline caliciviruses (FCVs) isolated from cats with virulent systemic disease possess in vitro phenotypes distinct from those of other FCV isolates

2007 ◽  
Vol 88 (2) ◽  
pp. 506-517 ◽  
Author(s):  
Robert J. Ossiboff ◽  
Alexander Sheh ◽  
Justine Shotton ◽  
Patricia A. Pesavento ◽  
John S. L. Parker
Keyword(s):  
Tissue Culture ◽  
Morphological Changes ◽  
Phylogenetic Analyses ◽  
Systemic Disease ◽  
Growth Conditions ◽  
Diagnostic Methods ◽  
Culture Cells ◽  
The Usa ◽  
Multiple Cycle

During the past decade, several outbreaks of severe systemic disease associated with Feline calicivirus (FCV) have occurred in the USA and the UK. This new disease has caused high mortality in the affected animals and has been termed virulent systemic (VS)-FCV disease. Currently, there are no genetic or in vitro diagnostic methods to distinguish viruses isolated from cases of VS-FCV disease from other isolates. Here, five in vitro properties, as well as the capsid and proteinase–polymerase (pro–pol) sequences, of a set of FCV isolates that included seven isolates from five distinct VS-FCV outbreaks (‘VS isolates’) were investigated. Although all of the FCV isolates investigated had similar kinetics of growth under single-cycle conditions, VS isolates infected tissue-culture cells more efficiently under multiple-cycle growth conditions. Moreover, it was found that cells infected with VS isolates showed cytopathic effects earlier than cells infected with non-VS isolates, although no difference in relative ATP levels were noted at times when morphological changes were first seen. Both VS- and other (non-VS) isolates of FCV demonstrated similar temperature stabilities. Phylogenetic analyses and alignments of the capsid and pro–pol regions of the genome did not reveal any conserved changes that correlated with virulence, and the VS isolates did not segregate into a unique clade. These results suggest that VS isolates have arisen independently several times since first being described and can spread more efficiently in tissue culture than other isolates when infected at low multiplicity.

Catecholamine-containing neurons in Remak's ganglion: a developmental and tissue culture study

1982 ◽  
Vol 11 (5) ◽  
pp. 763-778 ◽  
Author(s):  
D. Cantino ◽  
A. Barasa ◽  
R. Guglielmone
Keyword(s):  
Tissue Culture ◽  
Culture Study
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