Sample Preparation for Single Cell Transcriptomics: Essential Oil Glands in Citrus Fruit Peel as an Example

Navel oranges are reportedly susceptible to postharvest peel disorders, including chilling injury and aging/stem end rind breakdown. These and other physiological disorders are sometimes given the common term “navel rind breakdown.” California citrus industry reports on recent incidences of navel rind breakdown suggested that some instances of this disorder were similar to “postharvest pitting,” a disorder that we have observed in a number of Florida citrus varieties. Thus, we decided to define the morphology and etiology of pitting of `Washington' navel orange (Citrus sinensis L.) peel. The disorder was characterized by the collapse of clusters of oil glands and was stimulated by wax application and high temperature (≥13 °C) storage. Internal ethanol levels of waxed fruit stored at high temperature (13 or 21 °C) were significantly higher among fruit that developed pitting than those that did not. The pitting observed in these studies is comparable to previously observed navel orange disorders that have occurred without known cause. Navel orange pitting is morphologically and etiologically distinct from chilling injury and aging/stem end rind breakdown, but is similar to postharvest pitting of Florida citrus fruit.

Download Full-text

Syzygium aromaticum (Clove) essential oil: an alternative for the sanitization of citrus fruit in packinghouses

Journal of Food Processing and Preservation ◽

10.1111/jfpp.15496 ◽

2021 ◽

Author(s):

Tiago Soraggi Battagin ◽

Mario Nicolas Caccalano ◽

Guilherme Dilarri ◽

Caio Felipe Cavicchia Zamuner ◽

Natália Alleoni ◽

...

Keyword(s):

Essential Oil ◽

Citrus Fruit ◽

Syzygium Aromaticum ◽

Clove Essential Oil

Download Full-text

Automation of single‐cell proteomic sample preparation

PROTEOMICS ◽

10.1002/pmic.202100198 ◽

2021 ◽

pp. 2100198

Author(s):

Michal Alexovič ◽

Ján Sabo ◽

Rémi Longuespée

Keyword(s):

Sample Preparation ◽

Single Cell

Download Full-text

PERBANDINGAN METODE MICROWAVE HYDRODISTILLATION (MH) DAN MICROWAVE HYDRODIFFUSION AND GRAVITY (MHG) UNTUK MENGEKSTRAK MINYAK ATSIRI DARI KULIT JERUK

Reka Buana Jurnal Ilmiah Teknik Sipil dan Teknik Kimia ◽

10.33366/rekabuana.v2i1.666 ◽

2017 ◽

Vol 2 (1) ◽

pp. 82

Author(s):

Ayu Chandra Kartika Fitri ◽

Fikka Kartika Widyastuti

Keyword(s):

Essential Oil ◽

Microwave Power ◽

Citrus Fruit ◽

Orange Peel ◽

Quality Standard ◽

Extraction Methods ◽

Oil Composition ◽

Citrus Peel ◽

Oxygenated Compounds ◽

Earth Gravity

One source of essential oil that has long been popular is a citrus fruit (Citrus aurantium). Results waste citrus peel around 500,000 tonnes per year. So the prospects for the utilization of waste orange peel are big enough to extract the essential oil. However, conventional extraction methods have drawbacks in terms of product quality, so to find solutions to these shortcomings, it is necessary to use the extraction method by Microwave Hydrodiffusion and Gravity (MHG). This method combines the use of microwave heating and physical phenomena (hydrodiffusi and earth gravity) at atmospheric pressure, without addition of organic solvents or water. In this study used fresh orange peel material 400 and 500 grams, two variable microwave power of 100 and 300 Watt and the extraction time for 15, 30, 45 and 60 minutes. To know the processing time and the microwave power effective method of MHG, oil composition was analyzed by GCMS to determine the amount of the fraction of oxygenated compounds. The larger of installed microwave power, the higher of operating temperature, so the %yield increases in a shorter time. From the comparison of energy consumed, the MHG method is give 30% more energy efficient than the Microwave Hydrodistillation (MHD) method. The use of microwaves does not change the physical properties of oil. The oil produced from the MHG method has a good quality because it has specific gravity and refractive index values that meet the EOA quality standard and has a greater oxygenated compounds content than the MHD method

Download Full-text

Developing Penicillium digitatum Management Strategies on Post-Harvest Citrus Fruits with Metabolic Components and Colonization of Bacillus subtilis L1-21

Journal of Fungi ◽

10.3390/jof8010080 ◽

2022 ◽

Vol 8 (1) ◽

pp. 80

Author(s):

Yongmei Li ◽

Mengyuan Xia ◽

Pengbo He ◽

Qiaoming Yang ◽

Yixin Wu ◽

...

Keyword(s):

Bacillus Subtilis ◽

Culture Filtrate ◽

Management Strategies ◽

Citrus Fruit ◽

Inhibition Zone ◽

Penicillium Digitatum ◽

Antifungal Compounds ◽

Fruit Peel ◽

Post Harvest

Citrus is among the most important plants in the fruit industry severely infected with pathogens. Citrus green mold caused by Penicillium digitatum is one of the most devastating diseases during post-harvest stages of citrus fruit. In this study, a potential endophyte Bacillus subtilis L1-21, isolated from healthy citrus plants, was assessed for its biocontrol activity against the pathogen P. digitatum. Based on an in vitro crosstalk assay, we suggested that B. subtilis L1-21 inhibits the pathogen with an inhibition zone of 3.51 ± 0.08 cm. Biocontrol efficacy was highest for the fermented culture filtrate of B. subtilis L1-21. Additionally, using GC-MS analysis, 13 compounds were detected in the extract of this endophyte. The culture filtrate in Landy medium could enlarge and deform pathogen spores and prevent them from developing into normal mycelium. Accordingly, the Landy culture filtrate of B. subtilis L1-21 was stable in the temperature range of 4–90 °C and pH of 3–11. Further, MALDI-TOF-MS for B. subtilis L1-21 detected surfactin, fengycin, bacillaene and bacilysin as potential antifungal compounds. GFP-tagged B. subtilis L1-21 easily colonized in citrus fruit peel and pulp, suggesting its role in eliminating the fungal pathogen. Altogether, it is highly expected that the production of antifungal compounds, and the colonization potential of B. subtilis L1-21 are required against the post-harvest P. digitatum pathogen on citrus fruit.

Download Full-text