3D Primary Culture Model to Study Human Mammary Development

2017 ◽  
pp. 139-147 ◽  
Author(s):  
Daniel H. Miller ◽  
Ethan S. Sokol ◽  
Piyush B. Gupta
Keyword(s):  
Primary Culture ◽  
Mammary Development ◽  
Culture Model

Calcium signaling in cultured human and rat duodenal enterocytes

1998 ◽  
Vol 275 (2) ◽  
pp. G296-G304 ◽  
Author(s):  
Catherine S. Chew ◽  
Bengt Säfsten ◽  
Gunnar Flemström
Keyword(s):  
Extracellular Matrix ◽  
Calcium Signaling ◽  
Primary Culture ◽  
Calcium Concentration ◽  
Duodenal Mucosa ◽  
Transient Increase ◽  
Active Movement ◽  
Culture Model ◽  
Columnar Cells ◽  
Sustained Increase

Vagal stimuli increase duodenal mucosal[Formula: see text] secretion and may provide anticipatory protection against acid injury, but duodenal enterocyte (duodenocyte) responses and cholinoceptor selectivity have not been defined. We therefore developed a stable primary culture model of duodenocytes from rats and humans. Brief digestion of scraped rat duodenal mucosa or human biopsies with collagenase/dispase yielded cells that attached to the extracellular matrix Matrigel within a few hours of plating. Columnar cells with villus enterocyte morphology that exhibited spontaneous active movement were evident between 1 and 3 days of culture. Rat duodenocytes loaded with fura 2 responded to carbachol with a transient increase in intracellular calcium concentration ([Ca2+]i), with an apparent EC50 of ∼3 μM. In a first type of signaling pattern, [Ca2+]ireturned to basal or near basal values within 3–5 min. In a second type, observed in cells with enlarged vacuoles characteristic of crypt cell morphology, the initial transient increase was followed by rhythmic oscillations. Human duodenocytes responded with a more sustained increase in [Ca2+]i, and oscillations were not observed. Rat as well as human duodenocytes also responded to CCK-octapeptide but not to vasoactive intestinal polypeptide. Equimolar concentrations (100 nM) of the subtype-independent muscarinic antagonist atropine and the M3 antagonist 4-diphenylacetoxy- N-methylpiperidine methiodide prevented the response to 10 μM carbachol, whereas the M1 antagonist pirenzepine and the M2 antagonists methoctramine and AF-DX 116BS had no effect at similar concentrations. Responses in rat and human duodenocytes were similar. A new agonist-sensitive primary culture model for rat and human duodenocytes has thus been established and the presence of enterocyte CCK and muscarinic M3 receptors demonstrated.

Microfluidic primary culture model of the lower motor neuron–neuromuscular junction circuit

2013 ◽  
Vol 218 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Katherine A. Southam ◽  
Anna E. King ◽  
Catherine A. Blizzard ◽  
Graeme H. McCormack ◽  
Tracey C. Dickson
Keyword(s):  
Neuromuscular Junction ◽  
Motor Neuron ◽  
Primary Culture ◽  
Lower Motor Neuron ◽  
Culture Model

scholarly journals Characterization of folate uptake by choroid plexus epithelial cells in a rat primary culture model

2007 ◽  
Vol 104 (6) ◽  
pp. 1494-1503 ◽  
Author(s):  
Jan B. Wollack ◽  
Benedette Makori ◽  
Stuti Ahlawat ◽  
Rajeth Koneru ◽  
Sonia C. Picinich ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Choroid Plexus ◽  
Culture Model ◽  
Choroid Plexus Epithelial

scholarly journals Primary culture model of peroxisome proliferator-activated receptor ? activity in prostate cancer cells

2003 ◽  
Vol 196 (1) ◽  
pp. 131-143 ◽  
Author(s):  
Yue Xu ◽  
Sunita Iyengar ◽  
Richard L. Roberts ◽  
Scott B. Shappell ◽  
Donna M. Peehl
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Primary Culture ◽  
Receptor Activity ◽  
Peroxisome Proliferator ◽  
Prostate Cancer Cells ◽  
Peroxisome Proliferator Activated Receptor ◽  
Culture Model

A primary culture model of rabbit conjunctival epithelial cells exhibiting tight barrier properties

1996 ◽  
Vol 15 (12) ◽  
pp. 1163-1169 ◽  
Author(s):  
Pratik Saha ◽  
Kwang-Jin Kim ◽  
Vincent H. L. Lee
Keyword(s):  
Epithelial Cells ◽  
Primary Culture ◽  
Barrier Properties ◽  
Culture Model ◽  
Conjunctival Epithelial Cells

scholarly journals The ubiquitylated protein profile of rat cortical neurons is altered in a primary culture model of stroke (728.12)

2014 ◽  
Vol 28 (S1) ◽  
Author(s):  
Michael Dominic Chua ◽  
Bryce Pasqualotto ◽  
Gordon Rintoul ◽  
Julian Guttman
Keyword(s):  
Primary Culture ◽  
Cortical Neurons ◽  
Protein Profile ◽  
Culture Model ◽  
Rat Cortical Neurons ◽  
Ubiquitylated Protein

scholarly journals Functional Cftr in crypt epithelium of organotypic enteroid cultures from murine small intestine

2012 ◽  
Vol 302 (10) ◽  
pp. C1492-C1503 ◽  
Author(s):  
Jinghua Liu ◽  
Nancy M. Walker ◽  
Matthew T. Cook ◽  
Akifumi Ootani ◽  
Lane L. Clarke
Keyword(s):  
Primary Culture ◽  
Three Dimensional ◽  
Muscarinic Agonist ◽  
Transmembrane Conductance Regulator ◽  
Crypt Epithelium ◽  
Culture Model ◽  
Dependent Cell ◽  
Physiological Studies ◽  
Well Differentiated

Physiological studies of intact crypt epithelium have been limited by problems of accessibility in vivo and dedifferentiation in standard primary culture. Investigations of murine intestinal stem cells have recently yielded a primary intestinal culture in three-dimensional gel suspension that recapitulates crypt structure and epithelial differentiation (Sato T, Vries RG, Snippert HJ, van de Wetering M, Barker N, Stange DE, Van Es JH, Abo A, Kujala P, Peters PJ, Clevers H. Nature 459: 262–265, 2009). We investigated the utility of murine intestinal crypt cultures (termed “enteroids”) for physiological studies of crypt epithelium by focusing on the transport activity of the cystic fibrosis transmembrane conductance regulator Cftr. Enteroids had multiple crypts with well-differentiated goblet and Paneth cells that degranulated on exposure to the muscarinic agonist carbachol. Modified growth medium provided a crypt proliferation rate, as measured by 5-ethynyl-2′-deoxyuridine labeling, which was similar to proliferation in vivo. Immunoblots demonstrated equivalent Cftr expression in comparisons of freshly isolated crypts with primary and passage 1 enteroids. Apparent enteroid differences in mRNA expression of other transporters were primarily associated with villous epithelial contamination of freshly isolated crypts. Microelectrode analysis revealed cAMP-stimulated membrane depolarization in enteroid epithelium from wild-type (WT) but not Cftr knockout (KO) mice. Morphological and microfluorimetric studies, respectively, demonstrated Cftr-dependent cell shrinkage and lower intracellular pH in WT enteroid epithelium in contrast to Cftr KO epithelium or WT epithelium treated with Cftr inhibitor 172. We conclude that crypt epithelium of murine enteroids exhibit Cftr expression and activity that recapitulates crypt epithelium in vivo. Enteroids provide a primary culture model that is suitable for physiological studies of regenerating crypt epithelium.

Induction of multiple heat shock proteins and neuroprotection in a primary culture model of familial amyotrophic lateral sclerosis

2006 ◽  
Vol 24 (2) ◽  
pp. 213-225 ◽  
Author(s):  
Zarah Batulan ◽  
David M. Taylor ◽  
Rebecca J. Aarons ◽  
Sandra Minotti ◽  
Mohammad M. Doroudchi ◽  
...  
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Primary Culture ◽  
Familial Amyotrophic Lateral Sclerosis ◽  
Culture Model ◽  
Shock Proteins ◽  
Lateral Sclerosis

Static Magnetic Stimulation Induces Changes in the Oxidative Status and Cell Viability Parameters in a Primary Culture Model of Astrocytes

2021 ◽  
Author(s):  
Caroline Crespo da Costa ◽  
Léo Anderson Meira Martins ◽  
André Peres Koth ◽  
Jéssica Marques Obelar Ramos ◽  
Fátima Theresinha Costa Rodrigues Guma ◽  
...  
Keyword(s):  
Cell Viability ◽  
Primary Culture ◽  
Magnetic Stimulation ◽  
Oxidative Status ◽  
Culture Model

A Novel In Vitro Primary Culture Model of the Lower Motor Neuron–Neuromuscular Junction Circuit

2015 ◽  
pp. 181-193 ◽  
Author(s):  
Katherine A. Southam ◽  
Anna E. King ◽  
Catherine A. Blizzard ◽  
Graeme H. McCormack ◽  
Tracey C. Dickson
Keyword(s):  
Neuromuscular Junction ◽  
Motor Neuron ◽  
Primary Culture ◽  
Lower Motor Neuron ◽  
Culture Model
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