Protocol for DNA Microarrays on Glass Slides

2019 ◽  
pp. 17-33
Author(s):  
Kathleen M. Eyster
Keyword(s):  
Dna Microarrays ◽  
Glass Slides

Silanization of silica and glass slides for DNA microarrays by impregnation and gas phase protocols: A comparative study

2011 ◽  
Vol 31 (2) ◽  
pp. 384-390 ◽  
Author(s):  
Magali Phaner-Goutorbe ◽  
Vincent Dugas ◽  
Yann Chevolot ◽  
Eliane Souteyrand
Keyword(s):  
Comparative Study ◽  
Gas Phase ◽  
Dna Microarrays ◽  
Glass Slides

scholarly journals Structure and Oligonucleotide Binding Efficiency of Differently Prepared Click Chemistry-Type DNA Microarray Slides Based on 3-Azidopropyltrimethoxysilane

Materials ◽  
2021 ◽  
Vol 14 (11) ◽  
pp. 2855
Author(s):  
Emilia Frydrych-Tomczak ◽  
Tomasz Ratajczak ◽  
Łukasz Kościński ◽  
Agnieszka Ranecka ◽  
Natalia Michalak ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Dna Microarrays ◽  
Structural Characteristics ◽  
X Ray ◽  
Force Microscopy ◽  
Atomic Force ◽  
Glass Slides ◽  
Surface Modified ◽  
Oligonucleotide Binding

The structural characterization of glass slides surface-modified with 3-azidopropyltrimethoxysilane and used for anchoring nucleic acids, resulting in the so-called DNA microarrays, is presented. Depending on the silanization conditions, the slides were found to show different oligonucleotide binding efficiency, thus, an attempt was made to correlate this efficiency with the structural characteristics of the silane layers. Atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and X-ray reflectometry (XRR) measurements provided information on the surface topography, chemical composition and thickness of the silane films, respectively. The surface for which the best oligonucleotides binding efficiency is observed, has been found to consist of a densely-packed silane layer, decorated with a high-number of additional clusters that are believed to host exposed azide groups.

scholarly journals Glass slides to DNA microarrays

2004 ◽  
Vol 7 (3) ◽  
pp. 20-26 ◽  
Author(s):  
Samuel D Conzone ◽  
Carlo G Pantano
Keyword(s):  
Dna Microarrays ◽  
Glass Slides

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

Detection of atrial natriuretic peptides (ANP) in rat atria by immunogold-silver staining (IGSS)

1994 ◽  
Vol 52 ◽  
pp. 304-305
Author(s):  
Robyn Rufner ◽  
Gerhard W. Hacker ◽  
Michele Forte ◽  
Nancyleigh E. Carson ◽  
Cristina Xenachis ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Silver Staining ◽  
Sodium Thiosulfate ◽  
Atrial Natriuretic Peptides ◽  
Paraffin Sections ◽  
Glass Slides ◽  
Metallic Salts ◽  
Wistar Kyoto ◽  
Right Atria

The use of immunogold-silver staining (IGSS) to enhance label penetration and Localization for immunocytochemistry or in situ hybridization utilizing a variety of metallic salts has been documented. In this morphological study, the effects of silver acetate, silver lactate and silver nitrate were evaluated for immunogold-labeling of a trial natriuretic peptides (ANP) in rat right atria.Eight Wistar Kyoto retired breeders were sedated with pentobarbital, perfused with either 4% paraformaldehyde (LM) or Karnovsky's fixative (EM), and right atria were dissected, processed, embedded in paraffin or epon, respectively and sectioned according to conventional methods. For light microscopy, an indirect IGSS method according to Hacker (3) was performed. Paraffin sections on glass slides were washed in ddH2O, immersed in Lugol's iodine, washed in ddH2O and treated with 2.5% aqueous sodium thiosulfate for 20 sec. After additional washes in ddH2O and TBS-0.1% fish gelatin, 10% normal goat serum (PBS with 1% BSA) was applied for 20 min before an overnight incubation at 4°C with a polyclonal α-ANP primary antibody (Peninsula Labs, 1:1000 in TBS/BSA).

Anti-Diabetic Activity of a Mineraloid Isolate, in vitro and in Genetically Diabetic Mice

2011 ◽  
Vol 81 (1) ◽  
pp. 34-42 ◽  
Author(s):  
Joel Deneau ◽  
Taufeeq Ahmed ◽  
Roger Blotsky ◽  
Krzysztof Bojanowski
Keyword(s):  
Dna Microarrays ◽  
Human Fibroblasts ◽  
Diabetic Animal ◽  
In Vitro Tests ◽  
Diabetic Mice ◽  
Fossil Material ◽  
Expression Of Genes ◽  
Enhanced Expression ◽  
Genetically Diabetic Mice

Type II diabetes is a metabolic disease mediated through multiple molecular pathways. Here, we report anti-diabetic effect of a standardized isolate from a fossil material - a mineraloid leonardite - in in vitro tests and in genetically diabetic mice. The mineraloid isolate stimulated mitochondrial metabolism in human fibroblasts and this stimulation correlated with enhanced expression of genes coding for mitochondrial proteins such as ATP synthases and ribosomal protein precursors, as measured by DNA microarrays. In the diabetic animal model, consumption of the Totala isolate resulted in decreased weight gain, blood glucose, and glycated hemoglobin. To our best knowledge, this is the first description ever of a fossil material having anti-diabetic activity in pre-clinical models.

Can DNA microarrays change diagnostics and therapy of lung cancer?

2006 ◽  
Vol 54 (S 1) ◽  
Author(s):  
HS Hofmann ◽  
A Simm ◽  
G Hansen ◽  
RJ Scheubel ◽  
RE Silber ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dna Microarrays ◽  
Diagnostics And Therapy
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