Seasonal Quantitative Dynamics of Periphyton Bacillariophyta on Experimental Glass Slides Exposed Monthly in a Coastal Seawater Area of the Crimea

2014 ◽  
Vol 16 (3) ◽  
pp. 229-236
Author(s):  
D. S. Balycheva
Keyword(s):  
Experimental Glass ◽  
Coastal Seawater ◽  
Glass Slides ◽  
The Crimea

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

Detection of atrial natriuretic peptides (ANP) in rat atria by immunogold-silver staining (IGSS)

1994 ◽  
Vol 52 ◽  
pp. 304-305
Author(s):  
Robyn Rufner ◽  
Gerhard W. Hacker ◽  
Michele Forte ◽  
Nancyleigh E. Carson ◽  
Cristina Xenachis ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Silver Staining ◽  
Sodium Thiosulfate ◽  
Atrial Natriuretic Peptides ◽  
Paraffin Sections ◽  
Glass Slides ◽  
Metallic Salts ◽  
Wistar Kyoto ◽  
Right Atria

The use of immunogold-silver staining (IGSS) to enhance label penetration and Localization for immunocytochemistry or in situ hybridization utilizing a variety of metallic salts has been documented. In this morphological study, the effects of silver acetate, silver lactate and silver nitrate were evaluated for immunogold-labeling of a trial natriuretic peptides (ANP) in rat right atria.Eight Wistar Kyoto retired breeders were sedated with pentobarbital, perfused with either 4% paraformaldehyde (LM) or Karnovsky's fixative (EM), and right atria were dissected, processed, embedded in paraffin or epon, respectively and sectioned according to conventional methods. For light microscopy, an indirect IGSS method according to Hacker (3) was performed. Paraffin sections on glass slides were washed in ddH2O, immersed in Lugol's iodine, washed in ddH2O and treated with 2.5% aqueous sodium thiosulfate for 20 sec. After additional washes in ddH2O and TBS-0.1% fish gelatin, 10% normal goat serum (PBS with 1% BSA) was applied for 20 min before an overnight incubation at 4°C with a polyclonal α-ANP primary antibody (Peninsula Labs, 1:1000 in TBS/BSA).

The Invasion of the Crimea

2009 ◽  
Author(s):  
Alexander William Kinglake
Keyword(s):  
The Crimea

The Invasion of the Crimea

2009 ◽  
Author(s):  
Alexander William Kinglake
Keyword(s):  
The Crimea

MODERN HYDROGRAPHIC AND WATER MANAGEMENT ZONING OF UKRAINE’S TERRITORY – IMPLEMENTATION OF THE WFD-2000/60/EC

2020 ◽  
Author(s):  
V.K. Khilchevskyi ◽  
Keyword(s):  
Water Management ◽  
Water Resources ◽  
River Basins ◽  
River Basin Management ◽  
The Black Sea ◽  
Main Function ◽  
River Basin Management Plans ◽  
Management Plans ◽  
The Crimea ◽  
Sea Coast

In contrast to the hydrological and hydrochemical zoning, hydrographic and water management zoning of Ukraine (2016) was created on a basin basis, taking into account the boundaries of river basins, and not physiographic zoning. The main function of hydrographic and water management zoning is water management. Primary is hydrographic zoning, and water management - based on it. The description of modern hydrographic zoning of the territory of Ukraine, approved in 2016 by the Verkhovna Rada of Ukraine and included in the Water Code of Ukraine is given. Hydrographic zoning is carried out for the development and implementation of river basin management plans. On the territory of Ukraine nine areas of river basins are allocated: Dnipro; Dnister; Danube; Southern Bug; Don; Vistula; rivers of the Crimea; rivers of the Black Sea coast; rivers of the Azov Sea coast 13 sub-basins are allocated in four river basins district. The water management zoning is described - the division of hydrographic units into water management areas, which is carried out for the development of water management balances. In the regions of the river basins in the territory of Ukraine allocated 132 water management areas, 59 of which are located in the Dnipro basin. About 9,000 bodies of surface water allocated for monitoring in Ukraine. Approved zoning is the implementation of the provisions of the EU Water Framework Directive 2000/60 / EC in the management of water resources in Ukraine. Modern hydrographic and water management zoning of the territory of Ukraine approximates the management of water resources of the state to European requirements.

Not by Firkowicz’s Fault: Daniel Chwolson’s Comic Blunders in Research of Hebrew Epigraphy of the Crimea and Caucasus, and their Impact on Jewish Studies in Russia

2020 ◽  
Vol 73 (4) ◽  
pp. 633-668
Author(s):  
Michael Nosonovsky ◽  
Dan Shapira ◽  
Daria Vasyutinsky-Shapira
Keyword(s):  
Soviet Union ◽  
Jewish Studies ◽  
Soviet Period ◽  
Political Pressure ◽  
Scholarly Community ◽  
Academic Literature ◽  
The Soviet Union ◽  
The Crimea ◽  
Huge Impact ◽  
Academic Argument

AbstractDaniel Chwolson (1819–1911) made a huge impact upon the research of Hebrew epigraphy from the Crimea and Caucasus. Despite that, his role in the more-than-a-century-long controversy regarding Crimean Hebrew tomb inscriptions has not been well studied. Chwolson, at first, adopted Abraham Firkowicz’s forgeries, and then quickly realized his mistake; however, he could not back up. Th e criticism by both Abraham Harkavy and German Hebraists questioned Chwolson’s scholarly qualifications and integrity. Consequently, the interference of political pressure into the academic argument resulted in the prevailing of the scholarly flawed opinion. We revisit the interpretation of these findings by Russian, Jewish, Karaite and Georgian historians in the 19th and 20th centuries. During the Soviet period, Jewish Studies in the USSR were in neglect and nobody seriously studied the whole complex of the inscriptions from the South of Russia / the Soviet Union. The remnants of the scholarly community were hypnotized by Chwolson’s authority, who was the teacher of their teachers’ teachers. At the same time, Western scholars did not have access to these materials and/or lacked the understanding of the broader context, and thus a number of erroneous Chwolson’s conclusion have entered academic literature for decades.

First Record of Ranatra linearis (Hemiptera, Nepidae) in Hypersaline Water Bodies of the Crimea

2016 ◽  
Vol 52 (2) ◽  
pp. 49-53 ◽  
Author(s):  
Ye. V. Anufriyeva ◽  
N. V. Shadrin
Keyword(s):  
First Record ◽  
Water Bodies ◽  
The Crimea ◽  
Hypersaline Water

SPECIAL ASPECTS OF THE OPERATION ON PROVISION SETTLEMENTS OF THE CRIMEA WITH FRESH DRINKING WATER

2015 ◽  
Vol 1 ◽  
pp. 87-91
Author(s):  
Dmitri Melnikov ◽  
◽  
Sergei Kopnyshev ◽  
Nikolai Sedykh ◽  
◽  
...  
Keyword(s):  
Drinking Water ◽  
The Crimea
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