Analysis of Adenovirus Infections in Synchronized Cells

2007 ◽  
pp. 83-101 ◽  
Author(s):  
David A. Ornelles ◽  
Robin N. Broughton-Shepard ◽  
Felicia D. Goodrum
Keyword(s):  
Synchronized Cells

scholarly journals CHANGES IN THE DNA SYNTHESIS PATTERN OF PARAMECIUM WITH INCREASED CLONAL AGE AND INTERFISSION TIME

1974 ◽  
Vol 61 (3) ◽  
pp. 591-598 ◽  
Author(s):  
Joan Smith-Sonneborn ◽  
Michael Klass
Keyword(s):  
Cell Cycle ◽  
Dna Synthesis ◽  
Unicellular Organism ◽  
Time Intervals ◽  
Synchronized Cells ◽  
Different Ages ◽  
Self Fertilization ◽  
Autoradiographic Analysis ◽  
S Period ◽  
First Time

The clonal age in paramecia refers to the total number of vegetative divisions a clone has undergone since its origin at autogamy (self-fertilization). As clonal age increases, the interfission time usually increases. The DNA synthesis pattern of cells of different ages was compared by autoradiographic analysis of the DNA synthesis of synchronized cells at various time intervals during the cell cycle (from one division to the next). The study showed that the G1 period (the lag in DNA synthesis post division) was constant, irrespective of interfission time or clonal age; but the duration of the DNA synthesis period increased with increased interfission time or clonal age. Therefore, we have shown for the first time that the G1 period is fixed, and the S period is increased in a eukaryotic unicellular organism as a function of interfission time and clonal age.

Nocodazole and cytochalasin D induce tetraploidy in mammalian cells

1984 ◽  
Vol 246 (1) ◽  
pp. C154-C156 ◽  
Author(s):  
G. W. Zieve
Keyword(s):  
Cell Division ◽  
Mammalian Cells ◽  
Cytochalasin D ◽  
Microtubule Assembly ◽  
Reversible Inhibitor ◽  
Cleavage Furrow ◽  
Synchronized Cells ◽  
Cells In Culture ◽  
Wide Range

Nocodazole, a rapidly reversible inhibitor of microtubule assembly is useful for preparing mammalian cells synchronized at all stages of mitosis. When synchronized cells are allowed to progress through mitosis in the presence of cytochalasin D, the cleavage furrow is inhibited and dikaryon cells are formed. These cells become homogeneous populations of stable mononuclear tetraploid cells after the following cell division. This procedure is applicable to a wide range of mammalian cells in culture.

scholarly journals Maximal binding levels of an H1 histone gene-specific factor in S-phase correlate with maximal H1 gene transcription.

1988 ◽  
Vol 8 (10) ◽  
pp. 4576-4578 ◽  
Author(s):  
S Dalton ◽  
J R Wells
Keyword(s):  
Cell Cycle ◽  
Nucleic Acids ◽  
Gene Transcription ◽  
S Phase ◽  
Histone Gene ◽  
Specific Factor ◽  
Synchronized Cells ◽  
Phase Regulation ◽  
G2 Phase ◽  
Binding Component

Levels of trans-acting factor (H1-SF) binding to the histone H1 gene-specific motif (5'-AAACACA-3' [L. S. Coles and J. R. E. Wells, Nucleic Acids Res. 13:585-594, 1985]) increase 12-fold from G1 to S-phase in synchronized cells and decrease again in G2 phase of the cell cycle. Since the H1 element is required for S-phase expression of H1 genes (S. Dalton and J. R. E. Wells, EMBO J. 7:49-56, 1988), it is likely that the increased levels of H1-SF binding component play an important role in S-phase regulation of H1 gene transcription.

Interferon inhibition of DNA synthesis and cell division

1972 ◽  
Vol 18 (2) ◽  
pp. 145-151 ◽  
Author(s):  
M. V. O'Shaughnessy ◽  
S. H. S. Lee ◽  
K. R. Rozee
Keyword(s):  
Cell Division ◽  
Antiviral Activity ◽  
Dna Synthesis ◽  
S Phase ◽  
Cell Suspensions ◽  
Depression Effect ◽  
Growth Depression ◽  
Synchronized Cells ◽  
Inhibition Of Dna Synthesis ◽  
New Protein

Using monodispersed cell suspensions, interferon preparations were shown to have both a lethal and a growth-depression effect in the same concentration range as that required for antiviral activity. In addition, synchronized cells treated with interferon respond by delaying their normal uptake of thymidine during S phase until after a period during which new protein is synthesized. Puromycin added during this period prevents both the synthesis of this protein and the subsequent synthesis of DNA.

Linear increase in glycolytic activity through the cell cycle of schizosaccharomyces pombe

1977 ◽  
Vol 24 (1) ◽  
pp. 69-79
Author(s):  
K. Hamburger ◽  
B. Kramhoft ◽  
S.B. Nissen ◽  
E. Zeuthen
Keyword(s):  
Selection Procedure ◽  
Linear Increase ◽  
Cyclic Heat Treatment ◽  
Cell Cycles ◽  
Synchronized Cells ◽  
Synchronous Cultures ◽  
Glycolytic Activity ◽  
Rate Of Increase ◽  
Cyclic Heat ◽  
Number Of Cycles

The glycolytic activity of 3 different synchronous system in S. pombe was studied. Synchronous cultures were produced by a selection procedure, by cyclic heat treatment, or by cloning cells from an exponentially multiplying culture. In all experiments a complex medium with 3% glucose was used. The glycolytic activity was recorded with a gasometric method, the gradient diver. A single cell in exponential growth or a small number of synchronized cells were placed in ampulla divers in which the cells progressed undistrubed through a number of cycles. An ampulla diver is in principle a narrow pipette by which a single or a few cells are removed from the mother culture. It serves next as an axenic growth chamber and at the same time as a gasometer. The divers were placed in linear saline density gradients and the gaseous exchanges taking place in the divers resulted in migration of the divers. The migration rate is a measure of the glycolytic activity of the cells. Our results show that the glycolytic activity increases in a linear fashion between sucessive divisions. The rate of increase doubles at each division. This true in all 3 synchronous systems, and we take this as an indication that the cell cycles of heat-synchronized cells do not deviate seriously from the normal.

Behavior of mitochondria in synchronized cells of Chlamydomonas reinhardtii (Chlorophyta)

1995 ◽  
Vol 108 (2) ◽  
pp. 499-507
Author(s):  
T. Ehara ◽  
T. Osafune ◽  
E. Hase
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Mitochondrial Membrane ◽  
Close Contact ◽  
Light Period ◽  
O2 Uptake ◽  
Synchronized Cells ◽  
Large Matrix ◽  
Type Form ◽  
Uptake Activity ◽  
Time Courses

Cells of Chlamydomonas reinhardtii Dangeard were synchronized under a 12 hour:12 hour light:dark regimen. Behavior of mitochondria in these cells was studied by fluorescence microscopy using a mitochondrial membrane-binding fluorescent dye, dimethylaminostyrylmethyl-pyridiniumiodine (DASMPI), as well as by electron microscopy. Following time courses of change in frequency of occurrence of five typical morphologies of mitochondria in synchronized cells, strikingly dynamic behavior of mitochondria was demonstrated. The five types are (A) a giant global mitochondrion with large matrix and peripherally localized cristae, a part of which is in close contact with the nucleus, (B) a mitochondrion composed of thick-corded bodies connected to each other, a part of which is in contact with the nucleus, (C) thin-corded forms with a few branches, (D) small lump forms scattered in the cytoplasm, and (E) stringy forms with intricate branchings extended throughout the cytoplasm. During the early half of the light period, changes of C-->B-->C-->D occur, while the inversely sequential changes of D-->C-->B-->C proceed during the later half of the light period. The appearance of the B-type mitochondrion is accompanied by a transient decrease of O2-uptake activity of cells. The early appearing B-type mitochondrion is temporarily turned into a giant A-type mitochondrion, concomitant with discharge of membranes into the cytoplasm and their retake by the A-type form in the process of reversion to B-type. In the reversion process, partitioning membranes are also formed in the large matrix of A-type mitochondrion.(ABSTRACT TRUNCATED AT 250 WORDS)

Effects of psoralen on replicon size and mean rate of DNA synthesis in partially synchronized cells of Pisum sativum L

1985 ◽  
Vol 158 (2) ◽  
pp. 500-508 ◽  
Author(s):  
D. Francis ◽  
Nanette D. Davies ◽  
J.A. Bryant ◽  
S.G. Hughes ◽  
D.R. Sibson ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Dna Synthesis ◽  
Synchronized Cells ◽  
Pisum Sativum L

scholarly journals Increased phosphorylation of vinculin on tyrosine does not occur during the release of stress fibers before mitosis in normal cells.

1983 ◽  
Vol 3 (3) ◽  
pp. 475-479 ◽  
Author(s):  
M J Rosok ◽  
L R Rohrschneider
Keyword(s):  
Cell Cycle ◽  
Stress Fiber ◽  
Stress Fibers ◽  
Major Event ◽  
Normal Cells ◽  
Synchronized Cells

We examined the extent of phosphorylation of vinculin on tyrosine after pulse labeling synchronized cells with 32PO4 at various stages of the cell cycle. No significant increase was observed in the incorporation of radioactivity into vinculin phosphotyrosine throughout the cell cycle, suggesting that the increased rate of phosphorylation of vinculin on tyrosine may not be the major event regulating stress fiber dissolution before mitosis.

Sign in / Sign up

Export Citation Format

Share Document