Production of Protein Arrays by Cell-Free Systems

2008 ◽  
pp. 207-215 ◽  
Author(s):  
Mingyue He ◽  
Michael J. Taussig
Keyword(s):  
Protein Arrays

Comparison of Conventional and Electron Spectroscopic Imaging in the Fixed Beam Electron Microscope of Ordered Protein Arrays

1985 ◽  
Vol 43 ◽  
pp. 74-75
Author(s):  
E. L. Buhle ◽  
U. Aebi
Keyword(s):  
Image Processing ◽  
Electron Microscope ◽  
High Resolution ◽  
Image Plane ◽  
Electron Spectroscopic Imaging ◽  
Protein Arrays ◽  
Beam Electron ◽  
Average Unit ◽  
Fixed Beam ◽  
Energy Components

CTEM brightfield images are formed by a combination of relatively high resolution elastically scattered electrons and unscattered and inelastically scattered electrons. In the case of electron spectroscopic images (ESI), the inelastically scattered electrons cause a loss of both contrast and spatial resolution in the image. In the case of ESI imaging on the Zeiss EM902, the transmited electrons are dispersed into their various energy components by passing them through a magnetic prism spectrometer; a slit is then placed in the image plane of the prism to select the electrons of a given energy loss for image formation. The purpose of this study was to compare CTEM with ESI images recorded on a Zeiss EM902 of ordered protein arrays. Digital image processing was employed to analyze the average unit cell morphologies of the two types of images.

scholarly journals Tracking the Antibody Immunome in Sporadic Colorectal Cancer by Using Antigen Self-Assembled Protein Arrays

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2718
Author(s):  
María González-González ◽  
José María Sayagués ◽  
Luis Muñoz-Bellvís ◽  
Carlos Eduardo Pedreira ◽  
Marcello L. R. de Campos ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Clinical Utility ◽  
Genetic Alterations ◽  
Western World ◽  
Sporadic Colorectal Cancer ◽  
Protein Arrays ◽  
Humoral Immune ◽  
Cellular Processes ◽  
Healthy Donors ◽  
Associated Proteins

Sporadic Colorectal Cancer (sCRC) is the third leading cause of cancer death in the Western world, and the sCRC patients presenting with synchronic metastasis have the poorest prognosis. Genetic alterations accumulated in sCRC tumor cells translate into mutated proteins and/or abnormal protein expression levels, which contribute to the development of sCRC. Then, the tumor-associated proteins (TAAs) might induce the production of auto-antibodies (aAb) via humoral immune response. Here, Nucleic Acid Programmable Protein Arrays (NAPPArray) are employed to identify aAb in plasma samples from a set of 50 sCRC patients compared to seven healthy donors. Our goal was to establish a systematic workflow based on NAPPArray to define differential aAb profiles between healthy individuals and sCRC patients as well as between non-metastatic (n = 38) and metastatic (n = 12) sCRC, in order to gain insight into the role of the humoral immune system in controlling the development and progression of sCRC. Our results showed aAb profile based on 141 TAA including TAAs associated with biological cellular processes altered in genesis and progress of sCRC (e.g., FSCN1, VTI2 and RPS28) that discriminated healthy donors vs. sCRC patients. In addition, the potential capacity of discrimination (between non-metastatic vs. metastatic sCRC) of 7 TAAs (USP5, ML4, MARCKSL1, CKMT1B, HMOX2, VTI2, TP53) have been analyzed individually in an independent cohort of sCRC patients, where two of them (VTI2 and TP53) were validated (AUC ~75%). In turn, these findings provided novel insights into the immunome of sCRC, in combination with transcriptomics profiles and protein antigenicity characterizations, wich might lead to the identification of novel sCRC biomarkers that might be of clinical utility for early diagnosis of the tumor. These results explore the immunomic analysis as potent source for biomarkers with diagnostic and prognostic value in CRC. Additional prospective studies in larger series of patients are required to confirm the clinical utility of these novel sCRC immunomic biomarkers.

scholarly journals Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling

Genomics ◽  
2005 ◽  
Vol 85 (3) ◽  
pp. 285-296 ◽  
Author(s):  
Claudia Gutjahr ◽  
Derek Murphy ◽  
Angelika Lueking ◽  
Andrea Koenig ◽  
Michal Janitz ◽  
...  
Keyword(s):  
Cdna Library ◽  
Th1 Cell ◽  
Protein Arrays ◽  
Antibody Screening ◽  
Serum Profiling ◽  
Mouse Protein

Systematic analysis of natural antibody responses to P. falciparum merozoite antigens by protein arrays

2013 ◽  
Vol 78 ◽  
pp. 148-158 ◽  
Author(s):  
Yan-Ting Fan ◽  
Yue Wang ◽  
Chuan Ju ◽  
Ting Zhang ◽  
Bin Xu ◽  
...  
Keyword(s):  
Antibody Responses ◽  
Natural Antibody ◽  
Protein Arrays ◽  
Systematic Analysis ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

Subtyping of breast cancer using reverse phase protein arrays

2014 ◽  
Vol 11 (6) ◽  
pp. 757-770 ◽  
Author(s):  
Johanna Sonntag ◽  
Kerstin Schlüter ◽  
Stephan Bernhardt ◽  
Ulrike Korf
Keyword(s):  
Breast Cancer ◽  
Reverse Phase ◽  
Protein Arrays ◽  
Phase Protein

scholarly journals Designed and biologically active protein lattices

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Shih-Ting Wang ◽  
Brian Minevich ◽  
Jianfang Liu ◽  
Honghu Zhang ◽  
Dmytro Nykypanchuk ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Biologically Active ◽  
Double Layers ◽  
Active Protein ◽  
Functional Protein ◽  
Protein Arrays ◽  
Iron Storage ◽  
Nanoscale Systems ◽  
Framework Design ◽  
2D And 3D

AbstractVersatile methods to organize proteins in space are required to enable complex biomaterials, engineered biomolecular scaffolds, cell-free biology, and hybrid nanoscale systems. Here, we demonstrate how the tailored encapsulation of proteins in DNA-based voxels can be combined with programmable assembly that directs these voxels into biologically functional protein arrays with prescribed and ordered two-dimensional (2D) and three-dimensional (3D) organizations. We apply the presented concept to ferritin, an iron storage protein, and its iron-free analog, apoferritin, in order to form single-layers, double-layers, as well as several types of 3D protein lattices. Our study demonstrates that internal voxel design and inter-voxel encoding can be effectively employed to create protein lattices with designed organization, as confirmed by in situ X-ray scattering and cryo-electron microscopy 3D imaging. The assembled protein arrays maintain structural stability and biological activity in environments relevant for protein functionality. The framework design of the arrays then allows small molecules to access the ferritins and their iron cores and convert them into apoferritin arrays through the release of iron ions. The presented study introduces a platform approach for creating bio-active protein-containing ordered nanomaterials with desired 2D and 3D organizations.

scholarly journals Protein kinase substrate identification on functional protein arrays

2008 ◽  
Vol 8 (1) ◽  
pp. 22 ◽  
Author(s):  
Lihao Meng ◽  
Gregory A Michaud ◽  
Janie S Merkel ◽  
Fang Zhou ◽  
Jing Huang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Functional Protein ◽  
Protein Arrays ◽  
Kinase Substrate ◽  
Substrate Identification

Ordered protein arrays as mesophases

1994 ◽  
Vol 1 (2) ◽  
pp. 75-78 ◽  
Author(s):  
H. Haas ◽  
H. Mohwald
Keyword(s):  
Protein Arrays

Quality control for a large-scale study using protein arrays and protein beads to measure immune response in serum and plasma

PROTEOMICS ◽  
2012 ◽  
Vol 12 (18) ◽  
pp. 2802-2807 ◽  
Author(s):  
Neha Ghevaria ◽  
Matthieu Visser ◽  
Ralf Hoffmann
Keyword(s):  
Immune Response ◽  
Quality Control ◽  
Large Scale ◽  
Protein Arrays ◽  
Large Scale Study
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