Methods for Human CD8+ T Lymphocyte Proteome Analysis

2008 ◽  
pp. 45-65 ◽  
Author(s):  
Lynne Thadikkaran ◽  
Nathalie Rufer ◽  
Corinne Benay ◽  
David Crettaz ◽  
Jean-Daniel Tissot
Keyword(s):  
T Lymphocyte ◽  
Proteome Analysis ◽  
Cd8 T Lymphocyte

scholarly journals CD8+ T lymphocyte is a main source of interferon-gamma production in Takayasu’s arteritis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yan-Long Ren ◽  
Tao-Tao Li ◽  
Wei Cui ◽  
Li-Min Zhao ◽  
Na Gao ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Takayasu’S Arteritis ◽  
Control Group ◽  
Takayasu's Arteritis ◽  
Cd8 T Lymphocyte ◽  
Healthy Control ◽  
Ifn Γ

AbstractInterferon-gamma (IFN-γ) is a cytokine involved in the pathogenesis of Takayasu’s arteritis (TAK). However, the source of IFN-γ in TAK patients is not fully clear. We aimed to investigate the source of IFN-γ in TAK. 60 TAK patients and 35 health controls were enrolled. The lymphocyte subsets of peripheral blood were detected by flow cytometry, cytokines were detected by Bio-plex. The correlation among lymphocyte subsets, cytokines and disease activity indexes was analyzed by person correlation. The level of serum IFN-γ in TAK patients was significantly increased (P < 0.05). The percentage of CD3+IFN-γ+ cells in peripheral blood CD3+ cells was significantly higher in TAK patients than that of healthy control group (P = 0.002). A higher proportion of CD3+CD8+IFN-γ+ cells/CD3+IFN-γ+ cells (40.23 ± 11.98% vs 35.12 ± 11.51%, P = 0.049), and a significantly lower CD3+CD4+IFN-γ+/ CD3+CD8+IFN-γ+ ratio (1.34 ± 0.62% vs 1.80 ± 1.33%, P = 0.027) were showed in the TAK group than that of control group. The CD3+CD8+IFN-γ+/CD3+IFN-γ+ ratio was positively correlated with CD3+IFN-γ+cells/ CD3+cells ratio (r = 0.430, P = 0.001), serum IFN-γ level (r = 0.318, P = 0.040) and IL-17 level (r = 0.326, P = 0.031). It was negatively correlated with CD3+CD4+IFN-γ+/CD3+IFN-γ+ ratio (r = − 0.845, P < 0.001). IFN-γ secreted by CD3+CD8 + T cells is an important source of serum IFN-γ in TAK patients.

scholarly journals A CD8+ T-lymphocyte-mediated and CD4+ T-lymphocyte-independent autoimmune diabetes of early onset in transgenic mice

Diabetologia ◽  
1994 ◽  
Vol 37 (12) ◽  
pp. 1277-1279 ◽  
Author(s):  
P. L. Herrera ◽  
D. M. Harlan ◽  
L. Fossati ◽  
S. Izui ◽  
J. Huarte ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
T Lymphocyte ◽  
Early Onset ◽  
Autoimmune Diabetes ◽  
Cd8 T Lymphocyte

scholarly journals A Nonprogressive Clinical Course in HIV‐Infected Individuals Expressing Human Leukocyte Antigen B57/5801 Is Associated with Preserved CD8+T Lymphocyte Responsiveness to the HW9 Epitope in Nef

2008 ◽  
Vol 197 (6) ◽  
pp. 871-879 ◽  
Author(s):  
Marjon Navis ◽  
Ingrid M. M. Schellens ◽  
Peter van Swieten ◽  
José A. M. Borghans ◽  
Frank Miedema ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
T Lymphocyte ◽  
Clinical Course ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Cd8 T Lymphocyte

scholarly journals Tertiary Mutations Stabilize CD8+ T Lymphocyte Escape-Associated Compensatory Mutations following Transmission of Simian Immunodeficiency Virus

2013 ◽  
Vol 88 (6) ◽  
pp. 3598-3604 ◽  
Author(s):  
B. J. Burwitz ◽  
H. L. Wu ◽  
J. S. Reed ◽  
K. B. Hammond ◽  
L. P. Newman ◽  
...  
Keyword(s):  
Simian Immunodeficiency Virus ◽  
T Lymphocyte ◽  
Cd8 T Lymphocyte ◽  
Immunodeficiency Virus ◽  
Compensatory Mutations
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