scholarly journals CD8+ T lymphocyte is a main source of interferon-gamma production in Takayasu’s arteritis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yan-Long Ren ◽  
Tao-Tao Li ◽  
Wei Cui ◽  
Li-Min Zhao ◽  
Na Gao ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Takayasu’S Arteritis ◽  
Control Group ◽  
Takayasu's Arteritis ◽  
Cd8 T Lymphocyte ◽  
Healthy Control ◽  
Ifn Γ

AbstractInterferon-gamma (IFN-γ) is a cytokine involved in the pathogenesis of Takayasu’s arteritis (TAK). However, the source of IFN-γ in TAK patients is not fully clear. We aimed to investigate the source of IFN-γ in TAK. 60 TAK patients and 35 health controls were enrolled. The lymphocyte subsets of peripheral blood were detected by flow cytometry, cytokines were detected by Bio-plex. The correlation among lymphocyte subsets, cytokines and disease activity indexes was analyzed by person correlation. The level of serum IFN-γ in TAK patients was significantly increased (P < 0.05). The percentage of CD3+IFN-γ+ cells in peripheral blood CD3+ cells was significantly higher in TAK patients than that of healthy control group (P = 0.002). A higher proportion of CD3+CD8+IFN-γ+ cells/CD3+IFN-γ+ cells (40.23 ± 11.98% vs 35.12 ± 11.51%, P = 0.049), and a significantly lower CD3+CD4+IFN-γ+/ CD3+CD8+IFN-γ+ ratio (1.34 ± 0.62% vs 1.80 ± 1.33%, P = 0.027) were showed in the TAK group than that of control group. The CD3+CD8+IFN-γ+/CD3+IFN-γ+ ratio was positively correlated with CD3+IFN-γ+cells/ CD3+cells ratio (r = 0.430, P = 0.001), serum IFN-γ level (r = 0.318, P = 0.040) and IL-17 level (r = 0.326, P = 0.031). It was negatively correlated with CD3+CD4+IFN-γ+/CD3+IFN-γ+ ratio (r = − 0.845, P < 0.001). IFN-γ secreted by CD3+CD8 + T cells is an important source of serum IFN-γ in TAK patients.

scholarly journals INTERFERON GAMMA EXPRESSION CD8+-T LYMPHOCYTE WITH ESAT-6-CFP-10 FUSION ANTIGEN STIMULATION BETWEEN ACTIVE TUBERCULOSIS, LATENT TUBERCULOSIS AND HEALTHY PEOPLE

2018 ◽  
Vol 24 (3) ◽  
Author(s):  
Holland Lydia Marpaung ◽  
Betty Agustina ◽  
Jusak Nugraha ◽  
Fransiska Fransiska
Keyword(s):  
Interferon Gamma ◽  
T Lymphocyte ◽  
Healthy Subjects ◽  
Vaccine Candidate ◽  
Healthy People ◽  
Antigen Stimulation ◽  
Cd8 T Lymphocyte ◽  
Latent Tb ◽  
Ifn Γ ◽  
Fusion Antigen

Tuberculosis (TB) is an infectious disease in the world causing a global problem. Vaccination with Purified Protein Derivative (PPD) still cannot prevent tuberculosis in Indonesia. Interferon-gamma (IFN-γ) produced by CD8+-T lymphocyte has an important role in eliminating Mycobacterium tuberculosis. The vaccine candidate antigenic test was done to observe the inducible ability of IFN-γ as a main protection cytokine. This study aim was to research the difference of IFN-γ expression CD8+-T lymphocyte percentage with ESAT-6-CFP-10 fusion antigen stimulations as a TB vaccine candidate. This research is a quasi-experimental study design in the laboratory by ESAT-6-CFP-10 fusion antigen-stimulated Peripheral Blood Mononuclear Cells (PBMC) culture in vitro in TB patients, latent TB, and healthy subjects’ groups. IFN-γ expression CD8+-T lymphocyte percentage were examined by flow cytometry BDFACSCalibur with results: without antigen fusion stimulation IFN-γ expression CD8+-T lymphocyte percentage mean (TB patients 2,560, latent TB 2,173, and healthy people 2,153) and with antigen fusion (TB patients 3,039, latent TB 2,471, and healthy people 2,405). There was no significant difference in fusion antigen stimulation PBMC between TB patients, latent TB, and healthy subjects’ group, and also within groups.

Establishment of CD4 and CD8 Lymphocyte subsets in a healthy HIV and Toxoplasma seronegative pregnant women in Libya

2021 ◽  
Vol 01 (1) ◽  
pp. 53-62
Author(s):  
Ahmad Amro
Keyword(s):  
Pregnant Women ◽  
T Lymphocyte ◽  
Lymphocyte Subsets ◽  
First Trimester ◽  
Control Group ◽  
Reference Ranges ◽  
Cell Counts ◽  
Normal Ranges ◽  
Cd8 T Lymphocyte ◽  
Cd8 Cell

Most of the diagnostic laboratories in Libya often depend on western textbooks for CD4+- and CD8+ T-lymphocyte reference values. In this paper, we established reference ranges for the Libyan Toxoplasma, HIV, HBV, and HCV seronegative healthy pregnant women in all trimesters of pregnancy, and compared them with a control group of non-pregnant women. Wholeblood samples were collected to provide normal ranges for CD4+ and CD8+ Lymphocyte subsets expressed as mean ± standard division. A total of 110 Libyan women who came from Tripoli and Zwara districts were investigated; 70 pregnant women (aged 27.8 ± 2.99, range 18-40 years old) and 40 non-pregnant women (aged 22.7±3.01, range 18-40 years old) were included as controls. All cases/controls were seronegative for toxoplasmosis, HIV, HBV and HCV. The CD4+ cell counts were 685±256 cell/#l at the first trimester (T1), 740±202 at T2, and 923±203 cell/#l at T3. While the CD8+ cell counts were 451±171 cell/#l at T1, 541±168 at T2, and 753±190 cell/#l at T3. The CD4:CD8 ratios were 1.5±0.64 at T1, 1.4±0.51 at T2, and 1.2±0.36 at T3. Moreover, the mean absolute CD4+ and CD8+ counts for the control group were 1001±232 cell/#l and 717±159 cell/#l respectively. Absolute counts of CD4+ and CD8+ cells in pregnant women were significantly lower as compared to controls (P<0.05). Statistically significant decrease in the CD4+ and CD8+ cell counts was reported during T1 (P<0.05). These values increased significantly during the T2, and was comparable to the controls during T3 (P>0.05). The absolute CD4+ and CD8+ cell counts decreased with age for both groups. Geographical variation was reported for the cell counts between Tripoli and Zwara district at T3. We established reference ranges of CD4+ and CD8+ T-lymphocytes for the Libyan healthy pregnant women and discussed their use as prognostic markers. Further cohorts with greater sample size may be required to define the stage of the disease in relation to the normal CD4+ and CD8+ T lymphocyte count subsets in the Libyan population.

scholarly journals Clinical efficacy of immunotherapy combined with chemotherapy in patients with advanced gastric cancer, its effect on nutritional status and Changes of peripheral blood T lymphocyte subsets

2021 ◽  
Vol 37 (7) ◽  
Author(s):  
Wen-wen Li ◽  
Jin Jiao ◽  
Zhi-yu Wang ◽  
Ya-ning Wei ◽  
Yuan-fang Zhang
Keyword(s):  
Gastric Cancer ◽  
Nutritional Status ◽  
Advanced Gastric Cancer ◽  
Clinical Efficacy ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Control Group ◽  
T Lymphocyte Subsets ◽  
Experimental Group

Objectives: To evaluate the clinical efficacy of immunotherapy combined with chemotherapy in patients with advanced gastric cancer and its effect on nutritional status and changes of peripheral blood T lymphocyte subsets. Methods: Sixty patients with locally advanced gastric cancer who were admitted by Affiliated Hospital of Hebei University from March 2020 to February 2021 were enrolled and randomly divided into two groups, with 30 cases in each group. The control group was treated with FOLFOX4 chemotherapy, while the experimental group was additively treated with cindilizumab on the basis of control group. The incidence of adverse reactions, clinical efficacy, improvement of nutritional and physical status, and changes in the levels of T lymphocyte subgroups in the two groups were compared and analyzed. Results: The total effective rate was 70% in the experimental group, which was better than 43.3% of the control group (p=0.04). The improvement rate of performance status (ECOG) score and nutritional indicators in the experimental group was significantly better than that in the control group (p<0.05). Moreover, the indicators of CD3+, CD4+, CD4+/CD8+ in the experimental group were significantly higher than those in the control group after treatment, with statistically significant differences (CD3+, p=0.01; CD4 +, p=0.02; CD4+/CD8+, p=0.01). Conclusion: Immunotherapy combined with chemotherapy has a significant effect on locally advanced gastric cancer patients, with significant improvement in physical strength and nutritional status, significant improvement in T lymphocyte function, and no obvious adverse reactions. It is worth promoting in clinical application. doi: https://doi.org/10.12669/pjms.37.7.4347 How to cite this:Li W, Jiao J, Wang Z, Wei Y, Zhang Y. Clinical efficacy of immunotherapy combined with chemotherapy in patients with advanced gastric cancer, its effect on nutritional status and Changes of peripheral blood T lymphocyte subsets. Pak J Med Sci. 2021;37(7):---------. doi: https://doi.org/10.12669/pjms.37.7.4347 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Neurobehavioral, Autonomic Nervous Function and Lymphocyte Subsets among Aluminum Electrolytic Workers

2003 ◽  
Vol 16 (2) ◽  
pp. 139-144 ◽  
Author(s):  
S.C. He ◽  
N. Qiao ◽  
W. Sheng
Keyword(s):  
T Lymphocyte ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Test Battery ◽  
Aluminum Concentration ◽  
Control Group ◽  
Autonomic Nervous Function ◽  
Autonomic Nervous ◽  
Function Test ◽  
Nervous Function

The purpose of our study is to determine the alteration of neurobehavioral parameters, autonomic nervous function and lymphocyte subsets in aluminum electrolytic workers of long-term aluminum exposure. Thirtythree men who were 35.16 ± 2.95 (mean ± S.D) years old occupationally exposed to aluminum for 14.91 ± 6.31 (mean ± S.D) years. Air AI level and urinary aluminum concentration were measured by means of graphite furnace atomic absorption spectrophotometer. Normal reference groups were selected from a flour plant. Neurobehavioral core test battery (NCTB) recommended by WHO was utilized. Autonomic nervous function test battery recommended by Ewing DJ was conducted on subjects. FAC SCAN was used to measure the lymphocyte subsets of peripheral blood. The mean air aluminum level in the workshop was 6.36 mg/m3, ranged from 2.90 to 11.38 mg/m3. Urinary aluminum of the AI electrolytic workers (40.08 ± 9.36 microgram/mg.cre) was obviously higher than that of control group (26.84 ± 8.93m/mg.cre). Neurobehavioral results showed that the scores of DSY, PAC and PA in AI electrolytic workers were significantly lower than those of control group, The scores of POMSC, POMSF and SRT among AI exposed workers were significantly augmented in relation to those of control group. The scores of SRT and SRTF were obviously decreased in AI exposed group compared to control group. Autonomic nervous function test results showed that R-R interval variability of max:min ratio of immediately standing up in AI electrolytic workers were decreased compare with the control group, while the BP-IS, HR-V, HR-DB, R30:15 had no significant change. Peripheral blood lymphocyte subsets test showed that CD4−CD8+ T lymphocyte in AI electrolytic workers increased. This study suggests that AI exposure exerts adverse effects on neurobehavioral performance, especially movement coordination and negative mood, and parasympathetic nervous function; moreover it increase CD4−CD8+ T lymphocyte subsets.

scholarly journals Unfavorable clinical implications of peripheral blood CD44+ and CD54+ lymphocytes in patients with lung cancer undergoing chemotherapy

2017 ◽  
Vol 33 (2) ◽  
pp. 208-214 ◽  
Author(s):  
Zhuanbo Luo ◽  
Yun Wang ◽  
Yanru Lou ◽  
Chao Cao ◽  
Richard Hubbard ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Clinical Stage ◽  
Unmet Need ◽  
Control Group ◽  
Lung Cancer Patients ◽  
Healthy Control

Background: There is an unmet need for identification of additional prognostic markers for lung cancer. The aim of this study was to identify novel clinical and immunological predictors of prognosis in lung cancer patients. Methods: Lymphocyte subsets CD3+, CD4+, CD8+, CD4+/8+, CD25+, CD69+, CD44+ and CD54+ were quantified in peripheral blood using flow cytometry, for 203 newly diagnosed lung cancer patients and 120 healthy controls. Results: The levels of CD3+, CD4+, CD8+, CD4+/CD8+ and CD69+ lymphocytes were significantly lower in patients with lung cancer compared with the healthy control group, while CD54+ and CD44+ lymphocytes were significantly higher. In stage III/IV patients with lymph node metastasis or distant metastasis, the levels of CD44+ and CD54+ lymphocytes were significantly increased compared with patients with stage I/II disease (p<0.05). The levels of CD44+ and CD54+ lymphocytes markedly reduced after chemotherapy, and follow-up analysis indicated that patients found without increase of CD44+ and CD54+ lymphocytes after chemotherapy had survival advantages. Independent predictors of survival in lung cancer patients included clinical stage (hazard ratio [HR] = 2.791; 95% confidence interval [95% CI], 1.42-3.54, p<0.001), CD44+ lymphocytes (HR = 1.282; 95% CI, 1.02-1.49, p = 0.002) and CD54+ lymphocytes (HR = 1.475; 95% CI, 1.22-1.73, p = 0.003). Elevated levels of CD44+ and CD54+ lymphocytes correlated with poor prognosis in lung cancer patients. Conclusions: Peripheral blood lymphocyte subsets in patients with lung cancer are different from those in healthy people, and circulating CD44+ and CD54+ lymphocytes seem to be a promising criterion to predict survival in lung cancer patients undergoing chemotherapy.

Immune Activation and Radioprotection by Propolis

2005 ◽  
Vol 33 (02) ◽  
pp. 231-240 ◽  
Author(s):  
Yasuyuki Takagi ◽  
In-Sook Choi ◽  
Takenori Yamashita ◽  
Takashi Nakamura ◽  
Ikukatsu Suzuki ◽  
...  
Keyword(s):  
T Cells ◽  
T Lymphocyte ◽  
Immune Activation ◽  
Peripheral Blood ◽  
Radioprotective Effect ◽  
Mouse Serum ◽  
Control Group ◽  
Activated Macrophages ◽  
Irradiation Group ◽  
Ifn Γ

In this study, we focused on immune stimulation by Propolis, and examined changes in the effect of irradiation after Propolis administration. We also examined the radioprotective effect of Propolis by observing its effect on the immune system. The effect of immune activation by Propolis was investigated by measuring the total immunoglobulin (Ig) G and IgM. The radioprotective effect of immune activation by Propolis was investigated by measuring the T-lymphocyte subsets in the peripheral blood of mice following whole body irradiation. Compared with the control group, the IgG was significantly reduced in the Propolis group, indicating that Propolis suppressed IgG production. ELISA revealed that the amount of IgM in mouse serum was significantly higher in the Propolis group as compared with the control group, indicating that Propolis increased IgM production. The number of CD4-positive cells was increased only in the Propolis group. Likewise, the number of CD4-positive cells increased by 81% in the Propolis with irradiation group compared with the irradiation group alone. Compared with the control group, the Propolis group increased CD8-positive cells. Compared with the irradiation alone group, CD8-positive cells were decreased by Propolis with irradiation group. Propolis activated macrophages to stimulate interferon (IFN)-γ production in association with the secondary activation of T-lymphocytes, resulting in a decrease in IgG and IgM production. Cytokines released from macrophages in mouse peripheral blood after Propolis administration activated helper T-cells to proliferate. In addition, activated macrophages in association with the secondary T-lymphocyte activation increased IFN-γ production and stimulated proliferation of cytotoxic T-cells and suppressor T-cells, indicating the activation of cell-mediated immune responses.

scholarly journals AB0436 CHANGES OF LYMPHOCYTE SUBSETS AND CLINICAL INDEXES IN PERIPHERAL BLOOD OF PATIENTS WITH ANTI-PHOSPHOLIPID SYNDROME

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1517.1-1517
Author(s):  
W. Niu ◽  
H. Y. Wen
Keyword(s):  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Laboratory Data ◽  
Healthy Control Group ◽  
Control Group ◽  
Primary Antiphospholipid Syndrome ◽  
Significant Difference ◽  
Healthy Control ◽  
Adverse Pregnancy ◽  
Anti Phospholipid Syndrome

Background:Anti-phospholipid Syndrome (APS) is a non-inflammatory autoimmune disease, which can be divided into primary and secondary. Changes in lymphocyte numbers in APS are caused by disruption of the immune balance.Objectives:The levels of lymphocyte subsets in peripheral blood of patients with anti-phospholipid syndrome were observed and their clinical indexes were analyzed.Methods:53 patients with anti-phospholipid syndrome (APS) were collected as the case group and divided into two groups of A, B according to whether primary and 50 health examiners as the healthy control group. The levels of peripheral lymphocyte subsets and laboratory data [erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), platelets (PLT)]levels in the three groups were analyzed. The measurement data is not subject to normal distribution using the Median-Quartile method for statistical description; multiple sample comparisons using the Kruskal-Wallis H test; p <0.05 as the difference is statistically significant.Results:(1) The rates of thrombosis and adverse pregnancy in the two case groups were significantly increased. In the two case groups, the ESR and CRP were higher than those in the healthy control group, and the CRP in group A was higher. (2) Compared with the healthy control group, the levels of CD8+T, CD4+T/CD8+T in case A group were increased,while the levels of total T,CD4+T, CD8+T,CD4+T/CD8+T,Th2 and Treg cells were decreased. However,there was no significant difference in Th17 cells level and Th17/Treg ratio compared with the healthy control group.Conclusion:APS patients were more prone to have thrombosis, adverse pregnancy and thrombocytopenia. The changes of lymphocyte subsets were seen in peripheral blood, and the primary and secondary had different directions and different degrees of manifestation.However, whether the secondary factors can aggravate the clinical indicators of APS is still unclear.References:[1]Simonin Laurent,Pasquier Elisabeth,Leroyer Christophe et al. Lymphocyte Disturbances in Primary Antiphospholipid Syndrome and Application to Venous Thromboembolism Follow-Up.[J].Clin Rev Allergy Immunol, 2017, 53: 14-27.doi:10.1007/s12016-016-8568-1.Disclosure of Interests:None declared

scholarly journals DNA Methylation Patterns of Interferon Gamma Gene Promoter and Serum Level in Pulmonary Tuberculosis: Their Role in Prognosis

2019 ◽  
Vol 16 (1) ◽  
pp. 0178
Author(s):  
Zayr Et al.
Keyword(s):  
Dna Methylation ◽  
Immune Response ◽  
Interferon Gamma ◽  
Gene Promoter ◽  
Innate Immune ◽  
Healthy Controls ◽  
Healthy Control ◽  
Ifn Γ ◽  
Methylation Patterns ◽  
Normal Controls

Tuberculosis (TB) still remains an important medical problem due to high levels of morbidity and mortality worldwide. A series of innate immune mechanisms that create a cytokine network control the pathogenesis of tuberculosis and this response has the capacity to modify the host genomic DNA structure through epigenetic mechanisms such as DNA methylation which could constantly alter the local gene expression pattern that can modulate the metabolism of the tissues and the immune-response. Interferon-gamma (IFN-γ) is an important pro-inflammatory cytokine regulator of the innate immune response to TB. This study aims to determine DNA methylation patterns of INF-γ gene promoter and measure serum IFN- γ level in newly diagnosed TB patients, relapse TB patients, and healthy control, in order to study the possibility of using these as a biomarker for the prognosis of TB stages in patients. The current case-control study included 66 patients with TB and 33 healthy control subjects. DNA was extracted from peripheral blood(PB) of included subjects and modified using sodium bisulfate specific kit. DNA methylation patterns of IFN-γ gene promoter was determine by using methylation specific polymerase chain reaction(MS-PCR).Serum IFN-γ level  was determined using enzyme linked immune-sorbent assay(ELISA). Results showed that percentages of DNA methylation patterns in normal controls, newly diagnostic TB patients and relapse TB patients were (63.3%, 18.2% and 21.2% respectively). Also, higher significant differences (P≤0.0001) of  un-methylated  IFN-γ gene promoter patterns in newly diagnostic TB patients  than  relapse TB patients comparison with healthy controls. The percentage of un-methylated DNA patterns in healthy controls, newly diagnostic TB patients and relapse TB patients were (9.9%, 39.4% and 51.5%, respectively). The mean of serum IFN-γ levels (pg/ml) for normal controls, newly diagnostic TB patients and relapse TB patients were (59.3± 13.8,75.8±24.3 and 69.6±18.7,respectively).In conclusion, there is a relative association between methylation of IFN-γ gene promoter and predisposing to TB progression.

scholarly journals Zinc supplementation has no effect on circulating levels of peripheral blood leucocytes and lymphocyte subsets in healthy adult men

2003 ◽  
Vol 89 (5) ◽  
pp. 695-703 ◽  
Author(s):  
Maxine Bonham ◽  
Jacqueline M. O'Connor ◽  
H. Denis Alexander ◽  
James Coulter ◽  
Paula M. Walsh ◽  
...  
Keyword(s):  
T Cells ◽  
Adverse Effects ◽  
Seasonal Variations ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Immune Status ◽  
Control Group ◽  
Dietary Intakes ◽  
Circulating Levels ◽  
Peripheral Blood Leucocytes

As a result of evidence documenting harmful effects of Zn supplementation on immune function and Cu status, thirty-eight men were recruited onto a Zn supplementation trial. The aim was to examine the effects of chronic Zn supplementation on circulating levels of peripheral blood leucocytes and lymphocyte subsets. Subjects (n 19) took 30 mg Zn/d for 14 weeks followed by 3 mg Cu/d for 8 weeks to counteract adverse effects, if any, of Zn supplementation on immune status resulting from lowered Cu status. A control group (n 19) took placebo supplements for the duration of the trial. Dietary intakes of Zn approximated 10 mg/d. Blood samples, taken throughout the trial, were assessed for full blood profiles and flow cytometric analyses of lymphocyte subsets. Putative indices of Cu status were also examined. Results indicate that there was no effect of Zn supplementation on circulating levels of peripheral blood leucocytes or on lymphocyte subsets. Cu status was also unaltered. Independent of supplement, there appeared to be seasonal variations in selected lymphocyte subsets in both placebo and supplemented groups. Alterations in circulating levels of B cells (cluster of differentiation (CD) 19), memory T cells (CD45RO) and expression of the intracellular adhesion molecule-1 (CD54) on T cells were observed. Findings indicated no adverse effects of Zn supplementation on immune status or Cu status and support the US upper level of Zn tolerance of 40 mg/d. The seasonal variations observed in lymphocyte subsets in the group as a whole could have implications for seasonal variability in the incidence of infectious diseases.

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