Serum Proteomics Using Mass Spectrometry

2009 ◽  
pp. 107-128 ◽  
Author(s):  
Brian L. Hood ◽  
David E. Malehorn ◽  
Thomas P. Conrads ◽  
William L. Bigbee
Keyword(s):  
Mass Spectrometry ◽  
Serum Proteomics

scholarly journals Mass Spectrometry-Based Proteomics in Molecular Diagnostics: Discovery of Cancer Biomarkers Using Tissue Culture

2013 ◽  
Vol 2013 ◽  
pp. 1-16 ◽  
Author(s):  
Debasish Paul ◽  
Avinash Kumar ◽  
Akshada Gajbhiye ◽  
Manas K. Santra ◽  
Rapole Srikanth
Keyword(s):  
Mass Spectrometry ◽  
Tissue Culture ◽  
Tumor Cell ◽  
Biomarker Discovery ◽  
Ca 125 ◽  
Clinical Practices ◽  
Tissue Samples ◽  
Clinical Biomarkers ◽  
Serum Proteomics ◽  
Wide Range

Accurate diagnosis and proper monitoring of cancer patients remain a key obstacle for successful cancer treatment and prevention. Therein comes the need for biomarker discovery, which is crucial to the current oncological and other clinical practices having the potential to impact the diagnosis and prognosis. In fact, most of the biomarkers have been discovered utilizing the proteomics-based approaches. Although high-throughput mass spectrometry-based proteomic approaches like SILAC, 2D-DIGE, and iTRAQ are filling up the pitfalls of the conventional techniques, still serum proteomics importunately poses hurdle in overcoming a wide range of protein concentrations, and also the availability of patient tissue samples is a limitation for the biomarker discovery. Thus, researchers have looked for alternatives, and profiling of candidate biomarkers through tissue culture of tumor cell lines comes up as a promising option. It is a rich source of tumor cell-derived proteins, thereby, representing a wide array of potential biomarkers. Interestingly, most of the clinical biomarkers in use today (CA 125, CA 15.3, CA 19.9, and PSA) were discovered through tissue culture-based system and tissue extracts. This paper tries to emphasize the tissue culture-based discovery of candidate biomarkers through various mass spectrometry-based proteomic approaches.

scholarly journals Serum biomarkers research of tourette’s syndrome children using proteomics techniques--a pilot study

2020 ◽  
Author(s):  
WenXiang He ◽  
YanNi Chen ◽  
Duan Wang ◽  
HaiQing Zhang ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Magnetic Bead ◽  
Serum Biomarkers ◽  
Tourette's Syndrome ◽  
Tourette’S Syndrome ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Children ◽  
Healthy Controls ◽  
Flight Mass Spectrometry ◽  
Serum Proteomics

Abstract Purpose:This study used proteomics to analyze the changes in serum proteomics between tourette’s syndrome (TS) children and healthy children in order to find serum biomarkers that can distinguish TS children from healthy children.Experimental design: We analyzed the serum proteome of 60 TS children and 30 healthy controls children using magnetic bead-based weak cation exchange (MB-WCX) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS). Next, we identified candidate biomarkers using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Candidate biomarkers were then validated using ELISA and western blotting.Results: 59 peaks were identified and the expression fold changes of seven peaks in the two groups were greater than 1.9. Two peaks (m/z: 6443.34 Da; m/z: 6642.05 Da;) tended to be upregulated, whilefive peaks (m/z: 863.13 Da; m/z: 2175.98 Da; m/z: 2191.841 Da; m/z: 2277.19 Da;m/z: 2293.11 Da) tended to be down-regulated in TS group. The peak for a 2191.84 Da peptide was identified as FGA (Isoform 1 of the Fibrinogen alpha chain precursor, FGA);The peak for a 2175.98 Da peptide was identified as PKM2 (Isoform M2 of Pyruvate kinase isozymes,PKM2);The peak for a 2277.19 Da peptide was identified as GAPDH (Glyceraldehyde-3-phosphate dehydrogenase,GAPDH);The peak for a 863.13 Da peptide was identified as PROC (Vitamin K-dependent protein C,PROC).Enzyme-linked immunosorbent assay (ELISA) analyses revealed that the expression of FGA and PKM2 were significantly higher in TS children than healthy controls children. Conclusion: FGA and PKM2 may be potential serum biomarkers to distinguish TS children from healthy children.

Unravelling in vitro variables of major importance for the outcome of mass spectrometry-based serum proteomics

2007 ◽  
Vol 847 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Mikkel West-Nørager ◽  
Christian Dahl Kelstrup ◽  
Christian Schou ◽  
Estrid V. Høgdall ◽  
Claus K. Høgdall ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Serum Proteomics

Mass Spectrometry-Based Serum Proteomics for Biomarker Discovery and Validation

2017 ◽  
pp. 451-466 ◽  
Author(s):  
Santosh D. Bhosale ◽  
Robert Moulder ◽  
Petri Kouvonen ◽  
Riitta Lahesmaa ◽  
David R. Goodlett
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Discovery ◽  
Serum Proteomics

T1010 Mass Spectrometry-Based Serum Proteomics Approach to Biomarker Discovery for Non-Invasive Diagnostics of Non-Alcoholic Steatohepatitis (NASH)

2009 ◽  
Vol 136 (5) ◽  
pp. A-845-A-846 ◽  
Author(s):  
Janice L. Theodorakis ◽  
Raj Vuppalanchi ◽  
Jinsam You ◽  
Kerry G. Bemis ◽  
Shawn C. Comella ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biomarker Discovery ◽  
Non Invasive ◽  
Alcoholic Steatohepatitis ◽  
Serum Proteomics ◽  
Proteomics Approach ◽  
Invasive Diagnostics

How SIMS microscopy can be used in medicine

1992 ◽  
Vol 50 (2) ◽  
pp. 1602-1603
Author(s):  
Philippe Fragu
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Applications ◽  
Secondary Ion Mass Spectrometry ◽  
Chemical Elements ◽  
Biological Applications ◽  
The Past ◽  
Potential Source ◽  
Secondary Ion ◽  
Chemical Integrity ◽  
Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

Imaging of Al-Li-Cu alloys with a Scanning Ion Microprobe

1990 ◽  
Vol 48 (2) ◽  
pp. 314-315
Author(s):  
K.K. Soni ◽  
D.B. Williams ◽  
J.M. Chabala ◽  
R. Levi-Setti ◽  
D.E. Newbury
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Equilibrium Phase ◽  
Icosahedral Symmetry ◽  
Ion Microprobe ◽  
X Ray ◽  
Cu Alloys ◽  
Two Phases ◽  
The University ◽  
Secondary Ion

In contrast to the inability of x-ray microanalysis to detect Li, secondary ion mass spectrometry (SIMS) generates a very strong Li+ signal. The latter’s potential was recently exploited by Williams et al. in the study of binary Al-Li alloys. The present study of Al-Li-Cu was done using the high resolution scanning ion microprobe (SIM) at the University of Chicago (UC). The UC SIM employs a 40 keV, ∼70 nm diameter Ga+ probe extracted from a liquid Ga source, which is scanned over areas smaller than 160×160 μm2 using a 512×512 raster. During this experiment, the sample was held at 2 × 10-8 torr.In the Al-Li-Cu system, two phases of major importance are T1 and T2, with nominal compositions of Al2LiCu and Al6Li3Cu respectively. In commercial alloys, T1 develops a plate-like structure with a thickness <∼2 nm and is therefore inaccessible to conventional microanalytical techniques. T2 is the equilibrium phase with apparent icosahedral symmetry and its presence is undesirable in industrial alloys.

Applications of Time-OF-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)

1990 ◽  
Vol 48 (2) ◽  
pp. 308-309
Author(s):  
Bruno Schueler ◽  
Robert W. Odom
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Structural Information ◽  
Time Of Flight ◽  
Biological Tissues ◽  
Polymer Surfaces ◽  
Tof Sims ◽  
Secondary Ions ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) provides unique capabilities for elemental and molecular compositional analysis of a wide variety of surfaces. This relatively new technique is finding increasing applications in analyses concerned with determining the chemical composition of various polymer surfaces, identifying the composition of organic and inorganic residues on surfaces and the localization of molecular or structurally significant secondary ions signals from biological tissues. TOF-SIMS analyses are typically performed under low primary ion dose (static SIMS) conditions and hence the secondary ions formed often contain significant structural information.This paper will present an overview of current TOF-SIMS instrumentation with particular emphasis on the stigmatic imaging ion microscope developed in the authors’ laboratory. This discussion will be followed by a presentation of several useful applications of the technique for the characterization of polymer surfaces and biological tissues specimens. Particular attention in these applications will focus on how the analytical problem impacts the performance requirements of the mass spectrometer and vice-versa.

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